Ralstonia phage Gervaise

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Gervaisevirus; Ralstonia virus Gervaise

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G5BAI7|A0A7G5BAI7_9CAUD Uncharacterized protein OS=Ralstonia phage Gervaise OX=2759728 GN=1Ca_00073 PE=4 SV=1
MM1 pKa = 7.63IEE3 pKa = 3.96HH4 pKa = 7.29TGPEE8 pKa = 4.28TLVGPFPFDD17 pKa = 3.3VPPARR22 pKa = 11.84PVGFIEE28 pKa = 4.56QWEE31 pKa = 4.12LDD33 pKa = 3.6QLKK36 pKa = 9.45NGCVARR42 pKa = 11.84VFPTKK47 pKa = 10.54DD48 pKa = 3.21EE49 pKa = 4.22PHH51 pKa = 6.75DD52 pKa = 3.74VALFTHH58 pKa = 7.06PEE60 pKa = 3.64ASATGLSDD68 pKa = 2.98GWKK71 pKa = 9.51QAAEE75 pKa = 4.19WVRR78 pKa = 11.84DD79 pKa = 3.65NYY81 pKa = 10.8QDD83 pKa = 3.72YY84 pKa = 11.63ANIASLVDD92 pKa = 3.44AMLARR97 pKa = 11.84ASAATACVACEE108 pKa = 4.16GSPSSEE114 pKa = 3.89NNPCAVCGRR123 pKa = 11.84AATVGEE129 pKa = 4.38ASVADD134 pKa = 3.87DD135 pKa = 6.4DD136 pKa = 4.57VFTWLEE142 pKa = 4.17TEE144 pKa = 4.51ISAVDD149 pKa = 3.65CRR151 pKa = 11.84YY152 pKa = 10.56HH153 pKa = 7.34GDD155 pKa = 3.23PSYY158 pKa = 11.86DD159 pKa = 3.24HH160 pKa = 7.31DD161 pKa = 4.99AYY163 pKa = 10.05WMRR166 pKa = 11.84EE167 pKa = 3.75RR168 pKa = 11.84VLKK171 pKa = 10.34LVKK174 pKa = 9.54EE175 pKa = 4.33AKK177 pKa = 10.21DD178 pKa = 3.5VFGKK182 pKa = 10.33VAQQQAEE189 pKa = 4.27PGAEE193 pKa = 3.83QQ194 pKa = 3.56

Molecular weight: 21.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G5BAE6|A0A7G5BAE6_9CAUD Muramidase domain-containing protein OS=Ralstonia phage Gervaise OX=2759728 GN=1Ca_00031 PE=4 SV=1
MM1 pKa = 7.74ANTKK5 pKa = 9.18PRR7 pKa = 11.84RR8 pKa = 11.84GDD10 pKa = 3.3RR11 pKa = 11.84ARR13 pKa = 11.84RR14 pKa = 11.84AATKK18 pKa = 7.61TTAAKK23 pKa = 10.62DD24 pKa = 3.53EE25 pKa = 4.81APWSHH30 pKa = 6.78GDD32 pKa = 3.09PTRR35 pKa = 11.84NVFINVRR42 pKa = 11.84FTEE45 pKa = 4.1PTILQMEE52 pKa = 4.29YY53 pKa = 10.76LIDD56 pKa = 3.78NKK58 pKa = 10.51VIFSKK63 pKa = 10.87AAFIRR68 pKa = 11.84AAVDD72 pKa = 3.44AACTRR77 pKa = 11.84EE78 pKa = 3.61IEE80 pKa = 4.19RR81 pKa = 11.84FRR83 pKa = 11.84KK84 pKa = 8.54VRR86 pKa = 11.84AYY88 pKa = 9.16MRR90 pKa = 11.84QLDD93 pKa = 4.0DD94 pKa = 3.61EE95 pKa = 4.53EE96 pKa = 5.77KK97 pKa = 10.02IRR99 pKa = 11.84HH100 pKa = 5.54RR101 pKa = 11.84KK102 pKa = 8.05KK103 pKa = 10.71

Molecular weight: 12.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

18787

31

4511

260.9

28.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.435 ± 0.462

0.719 ± 0.203

6.159 ± 0.209

6.398 ± 0.392

3.05 ± 0.146

8.517 ± 0.374

1.9 ± 0.144

4.673 ± 0.243

4.695 ± 0.362

6.968 ± 0.191

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.667 ± 0.124

3.641 ± 0.238

4.886 ± 0.172

4.093 ± 0.201

6.984 ± 0.33

5.429 ± 0.235

5.69 ± 0.317

6.238 ± 0.168

1.315 ± 0.165

2.544 ± 0.098
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski