Reovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1)

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Orthoreovirus; Mammalian orthoreovirus; Mammalian orthoreovirus 1

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9PY83|MUNS_REOVL Protein mu-NS OS=Reovirus type 1 (strain Lang) OX=10884 GN=M3 PE=1 SV=1
MM1 pKa = 7.34ANVWGVRR8 pKa = 11.84LADD11 pKa = 3.82SLSSPTIEE19 pKa = 3.86TRR21 pKa = 11.84TRR23 pKa = 11.84HH24 pKa = 3.99YY25 pKa = 9.49TLRR28 pKa = 11.84DD29 pKa = 3.48FCSDD33 pKa = 3.19LDD35 pKa = 3.8AVAGKK40 pKa = 9.88EE41 pKa = 3.64PWRR44 pKa = 11.84PLRR47 pKa = 11.84NQRR50 pKa = 11.84TNDD53 pKa = 2.92IVAVQLFRR61 pKa = 11.84PLQGLVLDD69 pKa = 3.96TQFYY73 pKa = 9.16GFPGIFSEE81 pKa = 4.3WEE83 pKa = 3.61QFIKK87 pKa = 10.76EE88 pKa = 4.07KK89 pKa = 10.95LRR91 pKa = 11.84VLKK94 pKa = 11.16YY95 pKa = 9.5EE96 pKa = 3.91VLRR99 pKa = 11.84IYY101 pKa = 10.2PISNYY106 pKa = 7.48NHH108 pKa = 5.75EE109 pKa = 4.33RR110 pKa = 11.84VNVFVANALVGAFLSNQAFYY130 pKa = 11.44DD131 pKa = 4.09LLPLLVINDD140 pKa = 3.59TMINDD145 pKa = 4.41LLGTGAALSQFFQSHH160 pKa = 6.06GEE162 pKa = 4.12VLEE165 pKa = 4.29VAAGRR170 pKa = 11.84KK171 pKa = 7.81YY172 pKa = 11.0LQMKK176 pKa = 9.9NYY178 pKa = 10.56SNDD181 pKa = 3.58DD182 pKa = 3.67DD183 pKa = 5.65DD184 pKa = 5.56PPLFAKK190 pKa = 10.55DD191 pKa = 3.38LSDD194 pKa = 3.66YY195 pKa = 11.6AKK197 pKa = 10.78AFYY200 pKa = 10.72SDD202 pKa = 3.24TFEE205 pKa = 4.06TLDD208 pKa = 3.44RR209 pKa = 11.84FFWTHH214 pKa = 6.75DD215 pKa = 3.09SSAGVLVHH223 pKa = 6.38YY224 pKa = 8.26DD225 pKa = 2.93KK226 pKa = 10.19PTNGNHH232 pKa = 6.48YY233 pKa = 10.47ILGTLTQMVSAPPHH247 pKa = 6.68IINATDD253 pKa = 3.4ALLLEE258 pKa = 4.75SCLEE262 pKa = 3.81QFAANVRR269 pKa = 11.84ARR271 pKa = 11.84PAQPVARR278 pKa = 11.84LDD280 pKa = 3.4QCYY283 pKa = 9.81HH284 pKa = 5.99LRR286 pKa = 11.84WGAQYY291 pKa = 10.87VGEE294 pKa = 4.72DD295 pKa = 3.3SLTYY299 pKa = 10.4RR300 pKa = 11.84LGVLSLLATNGYY312 pKa = 9.17QLARR316 pKa = 11.84PIPKK320 pKa = 9.77QLTNRR325 pKa = 11.84WLSSFVSQVMSDD337 pKa = 3.82GVNEE341 pKa = 4.18TPLWPQEE348 pKa = 4.03RR349 pKa = 11.84YY350 pKa = 8.46VQIAYY355 pKa = 9.43DD356 pKa = 3.79SPSVVDD362 pKa = 4.22GATHH366 pKa = 5.84YY367 pKa = 10.95GYY369 pKa = 10.87VRR371 pKa = 11.84RR372 pKa = 11.84NQLRR376 pKa = 11.84LGMRR380 pKa = 11.84VSALQSLSDD389 pKa = 3.87TPAPIQWLPQYY400 pKa = 9.62TIEE403 pKa = 4.35QAAVDD408 pKa = 4.28EE409 pKa = 4.68GDD411 pKa = 3.96LMVSRR416 pKa = 11.84LTQLPLRR423 pKa = 11.84PDD425 pKa = 3.46YY426 pKa = 11.39GSIWVGDD433 pKa = 3.8ALSYY437 pKa = 10.3YY438 pKa = 10.06VDD440 pKa = 3.67YY441 pKa = 11.11NRR443 pKa = 11.84SHH445 pKa = 6.66RR446 pKa = 11.84VVLSSEE452 pKa = 4.47LPQLPDD458 pKa = 2.96TYY460 pKa = 11.3FDD462 pKa = 3.83GDD464 pKa = 3.65EE465 pKa = 4.0QYY467 pKa = 11.5GRR469 pKa = 11.84SLFSLARR476 pKa = 11.84KK477 pKa = 9.63IGDD480 pKa = 3.16RR481 pKa = 11.84SLIKK485 pKa = 10.01DD486 pKa = 3.53TAVLKK491 pKa = 10.43HH492 pKa = 6.56AYY494 pKa = 8.89QAIDD498 pKa = 3.52PNTGKK503 pKa = 10.59EE504 pKa = 3.98YY505 pKa = 10.86LRR507 pKa = 11.84AGQSVAYY514 pKa = 9.34FGASAGHH521 pKa = 6.32SGADD525 pKa = 3.12QPLVIEE531 pKa = 5.03PWTQGKK537 pKa = 9.79ISGVPPPSSVRR548 pKa = 11.84QFGYY552 pKa = 10.79DD553 pKa = 3.29VAKK556 pKa = 10.57GAIVDD561 pKa = 4.37LARR564 pKa = 11.84PFPSGDD570 pKa = 3.41YY571 pKa = 10.22QFVYY575 pKa = 10.9SDD577 pKa = 3.15VDD579 pKa = 3.69QVVDD583 pKa = 3.69GHH585 pKa = 7.25DD586 pKa = 4.11DD587 pKa = 3.62LSISSGLVEE596 pKa = 5.07SLLDD600 pKa = 3.67SCMHH604 pKa = 5.96ATSPGGSFVMKK615 pKa = 10.24INFPTRR621 pKa = 11.84TVWHH625 pKa = 6.39YY626 pKa = 10.73IEE628 pKa = 5.6QKK630 pKa = 9.72ILPNITSYY638 pKa = 10.97MLIKK642 pKa = 10.53PFVTNNVEE650 pKa = 4.06LFFVAFGVHH659 pKa = 4.77QQSALTWTSGVYY671 pKa = 10.02FFLVDD676 pKa = 3.09HH677 pKa = 7.04FYY679 pKa = 10.67RR680 pKa = 11.84YY681 pKa = 7.46EE682 pKa = 3.98TLSTISRR689 pKa = 11.84QLPSFGYY696 pKa = 10.34VDD698 pKa = 4.85DD699 pKa = 5.1GSSVTGIEE707 pKa = 4.38MISLEE712 pKa = 4.21NPGFSNMTQAARR724 pKa = 11.84VGISGLCANVGNARR738 pKa = 11.84KK739 pKa = 9.63LISIHH744 pKa = 5.95EE745 pKa = 4.25SHH747 pKa = 6.97GARR750 pKa = 11.84VLTITSRR757 pKa = 11.84RR758 pKa = 11.84SPASARR764 pKa = 11.84RR765 pKa = 11.84KK766 pKa = 9.76ARR768 pKa = 11.84LRR770 pKa = 11.84YY771 pKa = 9.46LPLVDD776 pKa = 3.93PRR778 pKa = 11.84SLEE781 pKa = 3.99VQARR785 pKa = 11.84TILPSNPVLFDD796 pKa = 3.64NVNGASPHH804 pKa = 5.4VCLTMMYY811 pKa = 9.72NFEE814 pKa = 4.24VSSAVYY820 pKa = 10.61DD821 pKa = 3.73GDD823 pKa = 3.94VVLDD827 pKa = 4.5LGTGPEE833 pKa = 3.96AKK835 pKa = 9.52ILEE838 pKa = 5.31LIPPTSPVTCVDD850 pKa = 3.28IRR852 pKa = 11.84PTAQPSGCWNVRR864 pKa = 11.84TTFLEE869 pKa = 3.91LDD871 pKa = 3.83YY872 pKa = 11.51LSDD875 pKa = 3.25GWITGIRR882 pKa = 11.84GDD884 pKa = 4.26IVTCMLSLGAAAAGKK899 pKa = 10.91SMTFDD904 pKa = 3.24AAFQQLVKK912 pKa = 10.94VLTKK916 pKa = 9.94STANVLLIQVNCPTDD931 pKa = 3.53VIRR934 pKa = 11.84TIKK937 pKa = 10.61GYY939 pKa = 10.98LEE941 pKa = 3.9IDD943 pKa = 3.54QTNKK947 pKa = 10.01RR948 pKa = 11.84YY949 pKa = 10.11RR950 pKa = 11.84FPKK953 pKa = 10.01FGRR956 pKa = 11.84DD957 pKa = 3.29EE958 pKa = 4.99PYY960 pKa = 10.99SDD962 pKa = 3.65MDD964 pKa = 3.74SLEE967 pKa = 4.52RR968 pKa = 11.84ICRR971 pKa = 11.84AAWPNCSITWVPLSYY986 pKa = 10.96DD987 pKa = 3.75LRR989 pKa = 11.84WTKK992 pKa = 10.47LALLEE997 pKa = 4.26STTLSSASVRR1007 pKa = 11.84IAEE1010 pKa = 4.42LMYY1013 pKa = 10.69KK1014 pKa = 9.79YY1015 pKa = 9.56MPVMRR1020 pKa = 11.84IDD1022 pKa = 3.18IHH1024 pKa = 5.82GLPMEE1029 pKa = 4.38KK1030 pKa = 9.71QGNFIVGQNCSLTIPGFNAQDD1051 pKa = 3.46VFNCYY1056 pKa = 9.73FNSALAFSTEE1066 pKa = 4.29DD1067 pKa = 3.47VNSAMIPQVTAQFDD1081 pKa = 3.99ANKK1084 pKa = 10.6GEE1086 pKa = 3.99WSLDD1090 pKa = 3.38MVFSDD1095 pKa = 3.53AGIYY1099 pKa = 8.99TMQALVGSNANPVSLGSFVVDD1120 pKa = 4.37SPDD1123 pKa = 4.22VDD1125 pKa = 3.82ITDD1128 pKa = 3.6AWPAQLDD1135 pKa = 3.67FTIAGTDD1142 pKa = 2.91VDD1144 pKa = 3.83ITVNPYY1150 pKa = 8.41YY1151 pKa = 10.89RR1152 pKa = 11.84LMAFVRR1158 pKa = 11.84IDD1160 pKa = 3.82GQWQIANPDD1169 pKa = 3.1KK1170 pKa = 11.08FQFFSSSTGTLVMNVKK1186 pKa = 10.44LDD1188 pKa = 3.18IADD1191 pKa = 4.4RR1192 pKa = 11.84YY1193 pKa = 9.2LLYY1196 pKa = 10.61YY1197 pKa = 9.84IRR1199 pKa = 11.84DD1200 pKa = 3.64VQSRR1204 pKa = 11.84DD1205 pKa = 3.04VGFYY1209 pKa = 9.9IQHH1212 pKa = 7.14PLQLLNTITLPTNEE1226 pKa = 5.26DD1227 pKa = 3.81LFLSAPDD1234 pKa = 3.44MRR1236 pKa = 11.84EE1237 pKa = 3.61WAVKK1241 pKa = 10.18EE1242 pKa = 4.15SGNTICILNSQGFVPPQDD1260 pKa = 3.07WDD1262 pKa = 3.74VLTDD1266 pKa = 4.23TISWSPSLPTYY1277 pKa = 9.43VVPPGDD1283 pKa = 3.62YY1284 pKa = 10.48TLTPLL1289 pKa = 4.79

Molecular weight: 143.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P07939|SIGM3_REOVL Outer capsid protein sigma-3 OS=Reovirus type 1 (strain Lang) OX=10884 GN=S4 PE=1 SV=1
MM1 pKa = 7.62APSQKK6 pKa = 10.13KK7 pKa = 9.22SRR9 pKa = 11.84KK10 pKa = 8.8SRR12 pKa = 11.84NKK14 pKa = 9.92SRR16 pKa = 11.84STLMISGLPILNSTDD31 pKa = 3.46LEE33 pKa = 4.39DD34 pKa = 4.93RR35 pKa = 11.84LLTSAIASQPLSQDD49 pKa = 2.44WVRR52 pKa = 11.84WIIDD56 pKa = 3.18LWVSRR61 pKa = 11.84VRR63 pKa = 11.84SRR65 pKa = 11.84NYY67 pKa = 8.01LTQVARR73 pKa = 11.84TLRR76 pKa = 11.84AYY78 pKa = 7.59PHH80 pKa = 6.78WVTEE84 pKa = 4.39SMLSNHH90 pKa = 6.3EE91 pKa = 3.81LTVWIRR97 pKa = 11.84SRR99 pKa = 11.84LISLDD104 pKa = 3.54EE105 pKa = 4.46HH106 pKa = 6.19PLWRR110 pKa = 11.84QMLEE114 pKa = 4.18AYY116 pKa = 8.83GQNN119 pKa = 3.24

Molecular weight: 13.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

1

12

8415

119

1289

701.3

78.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.891 ± 0.436

1.331 ± 0.177

6.298 ± 0.336

4.444 ± 0.307

3.482 ± 0.199

5.443 ± 0.33

1.996 ± 0.257

5.561 ± 0.233

3.803 ± 0.383

9.507 ± 0.32

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.351 ± 0.293

4.088 ± 0.41

5.431 ± 0.508

4.765 ± 0.348

5.704 ± 0.259

8.307 ± 0.312

6.322 ± 0.459

7.344 ± 0.373

1.557 ± 0.22

3.375 ± 0.328
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski