Micromonospora echinospora (Micromonospora purpurea)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6415 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C4ZK18|A0A1C4ZK18_MICEC Transposase OS=Micromonospora echinospora OX=1877 GN=GA0070618_5381 PE=4 SV=1
MM1 pKa = 7.6TDD3 pKa = 3.65AGSLSDD9 pKa = 3.62GALRR13 pKa = 11.84NPGVVSIWAGHH24 pKa = 6.62LPDD27 pKa = 5.12EE28 pKa = 5.11DD29 pKa = 5.2AFDD32 pKa = 5.3DD33 pKa = 4.34YY34 pKa = 12.09VSFRR38 pKa = 11.84YY39 pKa = 9.62PDD41 pKa = 5.43DD42 pKa = 5.33DD43 pKa = 5.69DD44 pKa = 3.6SWSPFTRR51 pKa = 11.84DD52 pKa = 3.88HH53 pKa = 6.14GLGWVDD59 pKa = 3.27EE60 pKa = 4.78DD61 pKa = 3.57QAEE64 pKa = 4.36AAWFADD70 pKa = 3.93GPYY73 pKa = 10.65SLVDD77 pKa = 3.22HH78 pKa = 7.03SYY80 pKa = 11.57GDD82 pKa = 3.91SFAPAADD89 pKa = 3.67EE90 pKa = 4.33DD91 pKa = 3.94LTRR94 pKa = 11.84RR95 pKa = 11.84FPDD98 pKa = 3.21ANSAYY103 pKa = 10.46LVYY106 pKa = 10.78DD107 pKa = 4.13LDD109 pKa = 4.4ASAVLVSGSPLLAFLGAYY127 pKa = 8.53PYY129 pKa = 10.85RR130 pKa = 11.84KK131 pKa = 9.82

Molecular weight: 14.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C4WAG1|A0A1C4WAG1_MICEC 50S ribosomal protein L9 OS=Micromonospora echinospora OX=1877 GN=rplI PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIISTRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.89GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6415

0

6415

2282014

37

14813

355.7

38.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.733 ± 0.045

0.744 ± 0.008

6.234 ± 0.027

5.019 ± 0.029

2.649 ± 0.016

9.439 ± 0.03

2.201 ± 0.013

2.917 ± 0.024

1.495 ± 0.021

10.453 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.564 ± 0.012

1.772 ± 0.019

6.52 ± 0.027

2.636 ± 0.018

8.616 ± 0.037

4.638 ± 0.02

6.367 ± 0.03

9.344 ± 0.029

1.589 ± 0.012

2.071 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski