Rhizobium sp. NFR07

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6556 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I1CAW4|A0A1I1CAW4_9RHIZ Uncharacterized protein OS=Rhizobium sp. NFR07 OX=1566262 GN=SAMN03159496_05495 PE=4 SV=1
MM1 pKa = 7.49NIKK4 pKa = 10.39SLLLGSAAALAAVSGAQAADD24 pKa = 4.21AIVAAEE30 pKa = 4.47PEE32 pKa = 3.93PMEE35 pKa = 4.1YY36 pKa = 10.8VRR38 pKa = 11.84VCDD41 pKa = 4.42AFGTGYY47 pKa = 10.47FYY49 pKa = 10.57IPGTEE54 pKa = 3.81TCLKK58 pKa = 9.41VGGRR62 pKa = 11.84VRR64 pKa = 11.84FDD66 pKa = 2.39AWYY69 pKa = 10.61GDD71 pKa = 3.72VYY73 pKa = 11.45DD74 pKa = 5.16NEE76 pKa = 5.74DD77 pKa = 3.34GTFTNTRR84 pKa = 11.84AEE86 pKa = 4.17IYY88 pKa = 10.33LDD90 pKa = 3.67SASDD94 pKa = 4.05TEE96 pKa = 4.31WGALKK101 pKa = 10.01TSIVARR107 pKa = 11.84FDD109 pKa = 4.25YY110 pKa = 10.86NPHH113 pKa = 6.56NDD115 pKa = 4.24DD116 pKa = 3.93GNGGAFNDD124 pKa = 3.76ATRR127 pKa = 11.84TRR129 pKa = 11.84LIGANIQLGGFLVGLADD146 pKa = 3.81SYY148 pKa = 11.66YY149 pKa = 11.18SSFINYY155 pKa = 10.03AGDD158 pKa = 3.74VINDD162 pKa = 3.73DD163 pKa = 4.22VISYY167 pKa = 10.93GPFEE171 pKa = 5.21LNQVAYY177 pKa = 10.84VFDD180 pKa = 4.61AGNGFGAYY188 pKa = 9.06IAVEE192 pKa = 4.16DD193 pKa = 5.19DD194 pKa = 3.69GQNDD198 pKa = 4.02DD199 pKa = 5.88DD200 pKa = 4.76IPGPASDD207 pKa = 4.09WVDD210 pKa = 3.29VTAGVKK216 pKa = 9.94YY217 pKa = 11.02DD218 pKa = 3.82NGTFMAGLVGGYY230 pKa = 9.84DD231 pKa = 3.43EE232 pKa = 5.18SVEE235 pKa = 4.04EE236 pKa = 4.28GAIKK240 pKa = 10.53ARR242 pKa = 11.84IGGTFGAFSAFILGGWNTDD261 pKa = 2.44GDD263 pKa = 4.57TLNKK267 pKa = 9.49YY268 pKa = 10.56APGDD272 pKa = 3.66SLGNPWGDD280 pKa = 2.9WALWAGAGYY289 pKa = 10.53KK290 pKa = 10.51FSDD293 pKa = 3.95KK294 pKa = 10.43IAANVQGSVTDD305 pKa = 4.06FGTWAITGNVQWTPVSGLLIQPEE328 pKa = 4.41LSYY331 pKa = 11.29TDD333 pKa = 3.57WEE335 pKa = 4.78DD336 pKa = 4.23GGDD339 pKa = 3.56QFGGLIRR346 pKa = 11.84IQRR349 pKa = 11.84TFF351 pKa = 2.87

Molecular weight: 37.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I1CUQ2|A0A1I1CUQ2_9RHIZ DNA-binding transcriptional regulator LysR family OS=Rhizobium sp. NFR07 OX=1566262 GN=SAMN03159496_06403 PE=3 SV=1
MM1 pKa = 7.15TEE3 pKa = 3.95IVAGSIDD10 pKa = 3.73PSGTRR15 pKa = 11.84ASSPASHH22 pKa = 6.57TEE24 pKa = 3.8YY25 pKa = 10.75VKK27 pKa = 10.42GWRR30 pKa = 11.84IAAICIVLLLINAFSQIDD48 pKa = 3.92RR49 pKa = 11.84ILPFILAEE57 pKa = 4.45KK58 pKa = 10.26IKK60 pKa = 10.98ADD62 pKa = 4.33LSLTDD67 pKa = 3.51TQMGLLTGIAFAVCYY82 pKa = 10.32ALLSLPLARR91 pKa = 11.84AADD94 pKa = 3.91RR95 pKa = 11.84GSPRR99 pKa = 11.84LVLVSCILLWSVMTTLGGFAASFMFLALTRR129 pKa = 11.84FGVAFGEE136 pKa = 4.5AGAVPAGHH144 pKa = 6.93ALIVRR149 pKa = 11.84KK150 pKa = 9.6IGHH153 pKa = 5.83EE154 pKa = 3.87RR155 pKa = 11.84RR156 pKa = 11.84GLAIGLFAMGIPLGTMFGFAAGGAIADD183 pKa = 3.52IFGWRR188 pKa = 11.84IVLMGAGAVGILIGLLTILIAGPTPALQSKK218 pKa = 9.95AGRR221 pKa = 11.84SEE223 pKa = 3.88PFLRR227 pKa = 11.84TSLEE231 pKa = 4.18MLSSPAFRR239 pKa = 11.84WMFTGAIFLGFAGAPFYY256 pKa = 11.17AFSIPFLIRR265 pKa = 11.84THH267 pKa = 6.51GLTATEE273 pKa = 4.34VGVSFGMLQGLMGIIGTLGGGRR295 pKa = 11.84WFDD298 pKa = 3.2HH299 pKa = 6.33AVRR302 pKa = 11.84SGTGRR307 pKa = 11.84VLGPPAILFLIASVTTTAALFAPAGWMSVALFVPAMLSFSFFLPWGFGAAHH358 pKa = 6.86LVAGPGRR365 pKa = 11.84QAQATSLMMIGSGLLGPALAPLFVGIVSDD394 pKa = 3.8AASAADD400 pKa = 3.53IPNGLGLGMLIVPVAAVSTGITLLIANRR428 pKa = 11.84RR429 pKa = 11.84IGAFLRR435 pKa = 11.84QPP437 pKa = 3.37

Molecular weight: 45.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6556

0

6556

1986648

21

10674

303.0

32.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.766 ± 0.041

0.77 ± 0.008

5.784 ± 0.031

5.814 ± 0.03

3.902 ± 0.022

8.348 ± 0.029

1.984 ± 0.015

5.75 ± 0.019

3.657 ± 0.028

9.834 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.652 ± 0.016

2.825 ± 0.024

4.888 ± 0.02

3.148 ± 0.019

6.595 ± 0.038

5.926 ± 0.022

5.441 ± 0.051

7.326 ± 0.027

1.281 ± 0.013

2.309 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski