Alistipes sp. CAG:514

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Rikenellaceae; Alistipes; environmental samples

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1898 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5B7X0|R5B7X0_9BACT TPR_REGION domain-containing protein OS=Alistipes sp. CAG:514 OX=1262696 GN=BN689_01294 PE=4 SV=1
MM1 pKa = 7.64PEE3 pKa = 4.64CYY5 pKa = 10.33VDD7 pKa = 3.41TNLIDD12 pKa = 4.12FLLDD16 pKa = 3.28KK17 pKa = 10.69QYY19 pKa = 9.99KK20 pKa = 8.56TDD22 pKa = 3.44ISEE25 pKa = 4.01LTQIFGGEE33 pKa = 3.82AQGII37 pKa = 4.07

Molecular weight: 4.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5BBA5|R5BBA5_9BACT Ribokinase OS=Alistipes sp. CAG:514 OX=1262696 GN=rbsK PE=3 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.39KK6 pKa = 9.59RR7 pKa = 11.84KK8 pKa = 6.9RR9 pKa = 11.84HH10 pKa = 5.35KK11 pKa = 10.35MSTHH15 pKa = 5.04KK16 pKa = 10.3RR17 pKa = 11.84KK18 pKa = 9.87KK19 pKa = 8.77RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.26NRR25 pKa = 11.84HH26 pKa = 4.71KK27 pKa = 10.87KK28 pKa = 9.36KK29 pKa = 10.77

Molecular weight: 3.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1898

0

1898

700056

29

2088

368.8

41.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.027 ± 0.053

1.279 ± 0.022

6.123 ± 0.04

6.292 ± 0.047

4.512 ± 0.037

7.712 ± 0.049

1.662 ± 0.02

6.208 ± 0.045

6.054 ± 0.049

8.701 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.678 ± 0.024

4.278 ± 0.04

3.94 ± 0.028

2.716 ± 0.025

5.286 ± 0.04

6.737 ± 0.053

5.423 ± 0.039

7.125 ± 0.044

1.254 ± 0.022

3.991 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski