Candidatus Izimaplasma sp. HR2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Tenericutes incertae sedis; Candidatus Izimaplasma; unclassified Candidatus Izimaplasma

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2222 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A094ILE8|A0A094ILE8_9BACT Uncharacterized protein OS=Candidatus Izimaplasma sp. HR2 OX=1541960 GN=KQ78_01144 PE=4 SV=1
MM1 pKa = 7.37IKK3 pKa = 10.17KK4 pKa = 8.23ITMALTVFILALTLTGCNGNSLSDD28 pKa = 3.58TEE30 pKa = 4.26QVLEE34 pKa = 4.32IKK36 pKa = 10.83NSISFDD42 pKa = 3.54LLEE45 pKa = 5.26IEE47 pKa = 4.52NNITIPSSDD56 pKa = 3.4IEE58 pKa = 4.65GVTLTWVSSKK68 pKa = 10.39PQYY71 pKa = 11.02LDD73 pKa = 2.71IDD75 pKa = 4.04GTVTIPTASMGDD87 pKa = 3.56QNVDD91 pKa = 3.23LSVTIEE97 pKa = 4.07FGTAVTTKK105 pKa = 9.43VFHH108 pKa = 6.14FTVIAPVVVIDD119 pKa = 3.69TALNTDD125 pKa = 3.26QTDD128 pKa = 3.58ALAMDD133 pKa = 4.6FTYY136 pKa = 11.14EE137 pKa = 4.0DD138 pKa = 3.49TDD140 pKa = 4.6FIASGIGEE148 pKa = 4.4VTLVSCIDD156 pKa = 3.44GDD158 pKa = 4.0TAIFTEE164 pKa = 5.15GGANFTVRR172 pKa = 11.84FLGVDD177 pKa = 3.73TPEE180 pKa = 3.55STYY183 pKa = 11.06RR184 pKa = 11.84FDD186 pKa = 3.55PWGKK190 pKa = 8.13AASMFTCDD198 pKa = 4.02KK199 pKa = 9.44LTNATTIVLEE209 pKa = 4.1YY210 pKa = 11.03DD211 pKa = 3.34PATTRR216 pKa = 11.84TEE218 pKa = 4.01GNGRR222 pKa = 11.84YY223 pKa = 9.34LSWIWYY229 pKa = 9.22DD230 pKa = 3.49GRR232 pKa = 11.84LLNLEE237 pKa = 5.0LIEE240 pKa = 4.15QAFSGSKK247 pKa = 10.18GVAGLKK253 pKa = 10.11YY254 pKa = 10.58EE255 pKa = 4.07NLFYY259 pKa = 9.04TTEE262 pKa = 3.93FTVQDD267 pKa = 3.33KK268 pKa = 10.86DD269 pKa = 3.5RR270 pKa = 11.84RR271 pKa = 11.84IWGEE275 pKa = 3.77DD276 pKa = 3.45DD277 pKa = 3.72PDD279 pKa = 3.57YY280 pKa = 11.16DD281 pKa = 3.94YY282 pKa = 11.87SLDD285 pKa = 3.58GVQITIEE292 pKa = 4.03EE293 pKa = 4.53LVTNQEE299 pKa = 4.48LYY301 pKa = 10.88VGTKK305 pKa = 9.32VVITGVVSRR314 pKa = 11.84NLEE317 pKa = 3.83GHH319 pKa = 7.25PYY321 pKa = 10.15LQVGDD326 pKa = 3.94YY327 pKa = 10.95AIYY330 pKa = 10.28LYY332 pKa = 10.08IGYY335 pKa = 7.99TYY337 pKa = 7.54TTKK340 pKa = 10.56LVEE343 pKa = 4.46GNEE346 pKa = 4.09VTISGLTPTYY356 pKa = 10.63HH357 pKa = 7.53PDD359 pKa = 3.26AEE361 pKa = 4.57TGSLQLTGFTRR372 pKa = 11.84EE373 pKa = 4.23HH374 pKa = 5.69ITVISVGNVVEE385 pKa = 4.14PEE387 pKa = 3.9IIEE390 pKa = 3.96VSEE393 pKa = 3.68IDD395 pKa = 3.93IYY397 pKa = 10.87TLSKK401 pKa = 10.79LITIEE406 pKa = 4.03NLTVTDD412 pKa = 4.49IYY414 pKa = 11.73VNDD417 pKa = 3.41ITGDD421 pKa = 3.64FTVTAEE427 pKa = 4.35DD428 pKa = 3.99SSDD431 pKa = 3.21NTISIRR437 pKa = 11.84INSGTDD443 pKa = 3.06ALLLPEE449 pKa = 5.35LFTVGTIFDD458 pKa = 3.67VTGPLGRR465 pKa = 11.84YY466 pKa = 7.01EE467 pKa = 4.57GNYY470 pKa = 9.4QVLLIYY476 pKa = 10.73YY477 pKa = 10.12DD478 pKa = 4.3DD479 pKa = 4.27VDD481 pKa = 4.95FKK483 pKa = 11.75

Molecular weight: 53.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A094J4E0|A0A094J4E0_9BACT Putative hydrolase OS=Candidatus Izimaplasma sp. HR2 OX=1541960 GN=KQ78_00426 PE=4 SV=1
MM1 pKa = 7.6AKK3 pKa = 10.21KK4 pKa = 10.68AMIVKK9 pKa = 8.18QQRR12 pKa = 11.84GSKK15 pKa = 10.13FSTRR19 pKa = 11.84EE20 pKa = 3.41YY21 pKa = 10.13TRR23 pKa = 11.84CEE25 pKa = 3.81RR26 pKa = 11.84CGRR29 pKa = 11.84PHH31 pKa = 7.24AVYY34 pKa = 10.5KK35 pKa = 10.51KK36 pKa = 10.43FKK38 pKa = 10.08LCRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.94LAYY49 pKa = 10.67LGQIPGVRR57 pKa = 11.84KK58 pKa = 10.13ASWW61 pKa = 2.78

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2222

0

2222

623788

30

2441

280.7

31.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.039 ± 0.06

0.837 ± 0.025

6.148 ± 0.048

7.118 ± 0.054

4.89 ± 0.044

6.107 ± 0.059

1.636 ± 0.024

9.841 ± 0.057

8.077 ± 0.089

9.399 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.548 ± 0.027

5.956 ± 0.052

2.689 ± 0.031

2.312 ± 0.03

3.414 ± 0.043

6.331 ± 0.048

5.506 ± 0.061

6.731 ± 0.051

0.693 ± 0.018

4.716 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski