Wenzhou tombus-like virus 17

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KH10|A0A1L3KH10_9VIRU RdRp catalytic domain-containing protein OS=Wenzhou tombus-like virus 17 OX=1923670 PE=4 SV=1
MM1 pKa = 7.56YY2 pKa = 9.38DD3 pKa = 3.08AQQPHH8 pKa = 5.75VLYY11 pKa = 10.16DD12 pKa = 3.45YY13 pKa = 10.17TGTGRR18 pKa = 11.84VYY20 pKa = 9.77IQSNFMVAFKK30 pKa = 10.3TGMCILVFGIMPILLADD47 pKa = 4.36YY48 pKa = 10.18ICPGYY53 pKa = 9.0NTVRR57 pKa = 11.84SYY59 pKa = 9.05QHH61 pKa = 5.93FWTFIKK67 pKa = 10.99YY68 pKa = 10.43VDD70 pKa = 3.42TDD72 pKa = 3.94EE73 pKa = 4.25IDD75 pKa = 3.25RR76 pKa = 11.84RR77 pKa = 11.84VDD79 pKa = 3.05INSASSLKK87 pKa = 9.46HH88 pKa = 5.7TDD90 pKa = 3.25PFLAWFRR97 pKa = 11.84YY98 pKa = 7.77EE99 pKa = 4.5RR100 pKa = 11.84NDD102 pKa = 3.2NGEE105 pKa = 4.35FYY107 pKa = 10.26PARR110 pKa = 11.84DD111 pKa = 3.58MLVSMEE117 pKa = 4.96LLAQCSHH124 pKa = 6.06AMQHH128 pKa = 5.7VLSDD132 pKa = 3.57DD133 pKa = 3.36KK134 pKa = 11.47TVFEE138 pKa = 5.22RR139 pKa = 11.84ILRR142 pKa = 11.84FCSTNCTVNLDD153 pKa = 3.84RR154 pKa = 11.84YY155 pKa = 10.54DD156 pKa = 3.68FLINGPRR163 pKa = 11.84QSIIHH168 pKa = 5.44NTAILSFSLYY178 pKa = 8.91KK179 pKa = 10.04QRR181 pKa = 11.84RR182 pKa = 11.84EE183 pKa = 3.88YY184 pKa = 11.01LKK186 pKa = 10.51EE187 pKa = 3.94FPFPQLQQ194 pKa = 3.1

Molecular weight: 22.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KH10|A0A1L3KH10_9VIRU RdRp catalytic domain-containing protein OS=Wenzhou tombus-like virus 17 OX=1923670 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 10.5RR3 pKa = 11.84MGSKK7 pKa = 10.13PPNSDD12 pKa = 3.79GILLSEE18 pKa = 4.62LKK20 pKa = 9.97TFVSEE25 pKa = 4.11WCSKK29 pKa = 10.49NLTPIPPSADD39 pKa = 3.01TSVSAWLDD47 pKa = 3.09KK48 pKa = 10.84CIYY51 pKa = 9.13PKK53 pKa = 9.58WRR55 pKa = 11.84KK56 pKa = 10.03DD57 pKa = 3.37YY58 pKa = 10.73LASLEE63 pKa = 4.36EE64 pKa = 3.96EE65 pKa = 4.58LKK67 pKa = 11.21VKK69 pKa = 10.72LNSTRR74 pKa = 11.84GWNDD78 pKa = 2.3ISKK81 pKa = 10.47VKK83 pKa = 10.72AFTKK87 pKa = 10.04MEE89 pKa = 4.14TYY91 pKa = 10.6PEE93 pKa = 4.19FKK95 pKa = 9.78HH96 pKa = 6.58ARR98 pKa = 11.84GIYY101 pKa = 9.61SRR103 pKa = 11.84SDD105 pKa = 3.15YY106 pKa = 11.06FKK108 pKa = 10.66IKK110 pKa = 9.48TGPIFKK116 pKa = 10.36LIEE119 pKa = 4.18KK120 pKa = 10.0EE121 pKa = 4.37VFKK124 pKa = 10.9HH125 pKa = 6.94DD126 pKa = 3.82YY127 pKa = 9.63FIKK130 pKa = 10.41KK131 pKa = 9.67IPIADD136 pKa = 3.97RR137 pKa = 11.84PSYY140 pKa = 9.69IQEE143 pKa = 3.72HH144 pKa = 6.45VYY146 pKa = 9.86QHH148 pKa = 5.71GAKK151 pKa = 10.28VIATDD156 pKa = 3.74YY157 pKa = 11.43SSFEE161 pKa = 4.03SAFVKK166 pKa = 10.63KK167 pKa = 10.59LMNSCEE173 pKa = 4.02MVMYY177 pKa = 10.38EE178 pKa = 5.36YY179 pKa = 10.1MIQNLPEE186 pKa = 5.01RR187 pKa = 11.84EE188 pKa = 4.06MFLKK192 pKa = 10.48CLNKK196 pKa = 10.03IKK198 pKa = 9.9TKK200 pKa = 10.55QDD202 pKa = 2.74INYY205 pKa = 9.62KK206 pKa = 10.27DD207 pKa = 3.26ININVDD213 pKa = 3.23ATRR216 pKa = 11.84MSGEE220 pKa = 4.05MCTSLGNGFSNLMFTLFLAHH240 pKa = 6.91KK241 pKa = 9.67NGVKK245 pKa = 10.0SLRR248 pKa = 11.84GVVEE252 pKa = 4.08GDD254 pKa = 3.54DD255 pKa = 4.19GLFSFYY261 pKa = 11.57GNLTQEE267 pKa = 4.95HH268 pKa = 6.52YY269 pKa = 10.73SQLGLIIKK277 pKa = 9.87LEE279 pKa = 3.97NVEE282 pKa = 5.15DD283 pKa = 3.72INSASFCGLIFDD295 pKa = 5.03EE296 pKa = 4.66VDD298 pKa = 3.29LSNVTDD304 pKa = 5.07PIEE307 pKa = 4.36VIQNFSWLNPRR318 pKa = 11.84YY319 pKa = 9.5ISSADD324 pKa = 3.57RR325 pKa = 11.84RR326 pKa = 11.84LKK328 pKa = 10.64EE329 pKa = 3.81LLRR332 pKa = 11.84CKK334 pKa = 10.35SLSLAHH340 pKa = 6.25QYY342 pKa = 9.43PGCPIIQSLAHH353 pKa = 5.9YY354 pKa = 9.19GLYY357 pKa = 7.81VTRR360 pKa = 11.84GITIRR365 pKa = 11.84LDD367 pKa = 3.87KK368 pKa = 10.83IHH370 pKa = 6.22MSNWEE375 pKa = 4.01RR376 pKa = 11.84EE377 pKa = 4.12QLILAVNDD385 pKa = 3.28KK386 pKa = 10.21TRR388 pKa = 11.84ILKK391 pKa = 10.32KK392 pKa = 10.46EE393 pKa = 3.56IGNNTRR399 pKa = 11.84LLVEE403 pKa = 4.47RR404 pKa = 11.84KK405 pKa = 9.6FGLTVEE411 pKa = 4.43DD412 pKa = 3.73QIEE415 pKa = 4.12LEE417 pKa = 4.56KK418 pKa = 11.46YY419 pKa = 9.6FDD421 pKa = 3.56SLKK424 pKa = 9.49TVQVLDD430 pKa = 3.91HH431 pKa = 6.69EE432 pKa = 4.79VLTRR436 pKa = 11.84YY437 pKa = 9.84CKK439 pKa = 10.22LQHH442 pKa = 5.85KK443 pKa = 10.21QYY445 pKa = 10.78FNDD448 pKa = 3.47YY449 pKa = 11.04CFVTDD454 pKa = 3.77VNSTNRR460 pKa = 11.84NYY462 pKa = 10.62PIMPRR467 pKa = 11.84RR468 pKa = 11.84DD469 pKa = 3.28FTVNNMSYY477 pKa = 8.3FTEE480 pKa = 4.54HH481 pKa = 6.67KK482 pKa = 10.25SEE484 pKa = 4.62SIRR487 pKa = 11.84KK488 pKa = 9.46SILLRR493 pKa = 4.18

Molecular weight: 57.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

687

194

493

343.5

40.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.639 ± 0.554

2.183 ± 0.218

5.968 ± 0.692

5.822 ± 1.227

5.531 ± 0.934

4.076 ± 0.259

2.62 ± 0.262

7.278 ± 0.32

7.278 ± 2.319

9.461 ± 0.387

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.057 ± 0.306

5.531 ± 0.494

3.639 ± 0.269

3.493 ± 0.921

5.531 ± 0.648

7.569 ± 0.767

5.24 ± 0.238

5.531 ± 0.077

1.164 ± 0.074

5.386 ± 0.729
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski