Salmonella phage St162

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Cornellvirus; unclassified Cornellvirus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A291AXA4|A0A291AXA4_9CAUD Putative tail tape measure protein OS=Salmonella phage St162 OX=2024312 GN=St162_gp56 PE=4 SV=1
MM1 pKa = 6.5VARR4 pKa = 11.84YY5 pKa = 9.06RR6 pKa = 11.84FNYY9 pKa = 7.46VQEE12 pKa = 4.15MFEE15 pKa = 4.17TDD17 pKa = 3.69AGEE20 pKa = 4.25QYY22 pKa = 11.04CEE24 pKa = 4.14TEE26 pKa = 3.49RR27 pKa = 11.84DD28 pKa = 3.19EE29 pKa = 4.15WVKK32 pKa = 11.35YY33 pKa = 10.22EE34 pKa = 5.22DD35 pKa = 3.74YY36 pKa = 11.67AEE38 pKa = 4.29LKK40 pKa = 10.48EE41 pKa = 4.67LLDD44 pKa = 3.9ALAYY48 pKa = 10.45SVLNYY53 pKa = 9.63PLSPDD58 pKa = 3.48TEE60 pKa = 4.14EE61 pKa = 3.98LAKK64 pKa = 10.75GVLGDD69 pKa = 3.66

Molecular weight:
8.12 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A291AX61|A0A291AX61_9CAUD Uncharacterized protein OS=Salmonella phage St162 OX=2024312 GN=St162_gp24 PE=4 SV=1
MM1 pKa = 7.52SLATDD6 pKa = 3.39VLKK9 pKa = 10.75RR10 pKa = 11.84ANADD14 pKa = 3.31LSPPSKK20 pKa = 10.13STWADD25 pKa = 3.25YY26 pKa = 10.34MGLAIRR32 pKa = 11.84KK33 pKa = 7.19KK34 pKa = 10.47AKK36 pKa = 9.65RR37 pKa = 11.84AAPKK41 pKa = 10.45GPRR44 pKa = 11.84LNRR47 pKa = 11.84KK48 pKa = 8.69KK49 pKa = 10.86GKK51 pKa = 6.12TVKK54 pKa = 10.27HH55 pKa = 5.21VAPKK59 pKa = 9.26HH60 pKa = 4.44EE61 pKa = 4.45HH62 pKa = 5.01VAGVAFNSRR71 pKa = 11.84NNTWDD76 pKa = 3.12AYY78 pKa = 10.49FYY80 pKa = 11.23NGVKK84 pKa = 9.82TIRR87 pKa = 11.84IGMFHH92 pKa = 5.74TQARR96 pKa = 11.84AIIARR101 pKa = 11.84RR102 pKa = 11.84IYY104 pKa = 9.65MYY106 pKa = 9.49WRR108 pKa = 11.84KK109 pKa = 10.11CGFDD113 pKa = 3.89NIPNKK118 pKa = 9.56PEE120 pKa = 3.47RR121 pKa = 11.84RR122 pKa = 11.84LYY124 pKa = 10.2TMRR127 pKa = 11.84NCSDD131 pKa = 3.09KK132 pKa = 11.38SS133 pKa = 3.42

Molecular weight:
15.3 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

12799

32

828

206.4

22.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.095 ± 0.543

1.102 ± 0.128

6.477 ± 0.237

6.274 ± 0.325

3.5 ± 0.178

7.782 ± 0.232

1.727 ± 0.182

5.055 ± 0.165

6.086 ± 0.337

7.813 ± 0.295

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.43 ± 0.128

4.563 ± 0.225

3.828 ± 0.207

3.992 ± 0.402

5.391 ± 0.23

5.68 ± 0.313

6.172 ± 0.403

7.016 ± 0.335

1.5 ± 0.169

3.516 ± 0.194

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski