Human feces-associated smacovirus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Porprismacovirus; unclassified Porprismacovirus

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I9WIN8|A0A1I9WIN8_9VIRU Replication associated protein OS=Human feces-associated smacovirus OX=1914415 PE=4 SV=1
MM1 pKa = 6.69ATNYY5 pKa = 10.66ASASYY10 pKa = 10.76QEE12 pKa = 4.29ILDD15 pKa = 3.88VNTVKK20 pKa = 11.08GNVTVIGIHH29 pKa = 5.93TPTGSTPVRR38 pKa = 11.84RR39 pKa = 11.84LSGFFAQFRR48 pKa = 11.84KK49 pKa = 9.57FRR51 pKa = 11.84YY52 pKa = 8.89KK53 pKa = 9.37GCSVQVVPAATLPADD68 pKa = 3.8PLQVGFEE75 pKa = 4.04AGEE78 pKa = 3.97NQIDD82 pKa = 3.95MRR84 pKa = 11.84DD85 pKa = 3.58MLNPILFHH93 pKa = 5.87GTHH96 pKa = 6.41GEE98 pKa = 4.12SLQVAWNNIFVNPNRR113 pKa = 11.84FDD115 pKa = 3.55GSASGNSSYY124 pKa = 9.56KK125 pKa = 9.97TGAGFDD131 pKa = 3.71ASDD134 pKa = 3.6LTFADD139 pKa = 3.7STNNPVEE146 pKa = 4.12TQYY149 pKa = 11.45YY150 pKa = 8.61QAITDD155 pKa = 3.78RR156 pKa = 11.84TWRR159 pKa = 11.84KK160 pKa = 9.59FGIQSTFKK168 pKa = 10.76LPFMSPRR175 pKa = 11.84VWKK178 pKa = 10.52ASTIYY183 pKa = 10.26PILPNGYY190 pKa = 7.94QQNGSMNTGDD200 pKa = 3.58TPLPEE205 pKa = 4.98GFLPPGTYY213 pKa = 9.77FDD215 pKa = 4.72NYY217 pKa = 10.42DD218 pKa = 3.45GRR220 pKa = 11.84GLTMPIDD227 pKa = 4.34GQGHH231 pKa = 6.43ILDD234 pKa = 4.16QKK236 pKa = 9.63WMSSGTTPLGWLPTVTMPRR255 pKa = 11.84VDD257 pKa = 3.95PNNATSVTLAPRR269 pKa = 11.84TLLPKK274 pKa = 9.91IFMGILVLPPSYY286 pKa = 10.47LQEE289 pKa = 3.97LYY291 pKa = 10.53FRR293 pKa = 11.84VIVTHH298 pKa = 5.85YY299 pKa = 10.86YY300 pKa = 9.28EE301 pKa = 4.74FKK303 pKa = 11.15GFTASLAPASEE314 pKa = 4.23MPDD317 pKa = 3.11VKK319 pKa = 10.23GTYY322 pKa = 9.78EE323 pKa = 3.93NAVKK327 pKa = 8.76QTAATSNVMALDD339 pKa = 3.77EE340 pKa = 4.83VEE342 pKa = 5.46DD343 pKa = 3.97STLEE347 pKa = 4.06SPTANVVRR355 pKa = 11.84ISDD358 pKa = 3.85GVFF361 pKa = 2.82

Molecular weight: 39.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I9WIN8|A0A1I9WIN8_9VIRU Replication associated protein OS=Human feces-associated smacovirus OX=1914415 PE=4 SV=1
MM1 pKa = 6.35VQAWMITGPRR11 pKa = 11.84SEE13 pKa = 4.42SIEE16 pKa = 3.92VEE18 pKa = 4.5EE19 pKa = 4.17IGGWQIEE26 pKa = 3.8IDD28 pKa = 3.35RR29 pKa = 11.84RR30 pKa = 11.84ISKK33 pKa = 9.72KK34 pKa = 10.61AIMIMLSKK42 pKa = 10.62NDD44 pKa = 3.71CKK46 pKa = 10.78KK47 pKa = 8.03WTIGQEE53 pKa = 4.03VGKK56 pKa = 10.15NGYY59 pKa = 8.23KK60 pKa = 9.35HH61 pKa = 4.12WQIRR65 pKa = 11.84VEE67 pKa = 4.1SSNTNFFKK75 pKa = 10.03WCKK78 pKa = 7.25QHH80 pKa = 6.66IPSAHH85 pKa = 5.08VEE87 pKa = 4.06KK88 pKa = 10.93AEE90 pKa = 4.13KK91 pKa = 10.64GVDD94 pKa = 3.16EE95 pKa = 4.52SRR97 pKa = 11.84YY98 pKa = 5.3EE99 pKa = 4.21TKK101 pKa = 10.38EE102 pKa = 3.86GQYY105 pKa = 8.52TQYY108 pKa = 10.01TDD110 pKa = 2.84RR111 pKa = 11.84VEE113 pKa = 3.89ILKK116 pKa = 9.93QRR118 pKa = 11.84FGKK121 pKa = 8.87MRR123 pKa = 11.84PNQIRR128 pKa = 11.84ALEE131 pKa = 4.11ALEE134 pKa = 4.1ATNDD138 pKa = 3.59RR139 pKa = 11.84EE140 pKa = 4.21VLVWYY145 pKa = 9.95DD146 pKa = 3.17EE147 pKa = 4.28SGNVGKK153 pKa = 10.44SWLTGALWEE162 pKa = 4.84RR163 pKa = 11.84GLAYY167 pKa = 9.61YY168 pKa = 9.54VPPTVDD174 pKa = 2.8SVKK177 pKa = 11.22AMVQWVASCYY187 pKa = 9.73QSGGWRR193 pKa = 11.84PYY195 pKa = 10.33IIIDD199 pKa = 4.57IPRR202 pKa = 11.84SWKK205 pKa = 9.69WSEE208 pKa = 3.68QLYY211 pKa = 10.73AAIEE215 pKa = 4.33SIKK218 pKa = 10.97DD219 pKa = 3.11GLVYY223 pKa = 9.39DD224 pKa = 4.17TRR226 pKa = 11.84YY227 pKa = 9.55HH228 pKa = 5.6AQCINIRR235 pKa = 11.84GVKK238 pKa = 10.09VLVLTNTKK246 pKa = 10.11PKK248 pKa = 10.19LDD250 pKa = 3.95KK251 pKa = 11.04LSADD255 pKa = 3.24RR256 pKa = 11.84WRR258 pKa = 11.84ICVFF262 pKa = 3.58

Molecular weight: 30.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

623

262

361

311.5

35.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.421 ± 0.433

0.963 ± 0.589

4.976 ± 0.247

5.297 ± 1.456

3.852 ± 1.449

7.384 ± 0.558

1.445 ± 0.051

6.1 ± 1.432

5.457 ± 1.832

6.421 ± 0.671

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.729 ± 0.273

4.815 ± 0.86

5.778 ± 1.698

4.494 ± 0.291

4.976 ± 0.943

6.902 ± 0.258

7.384 ± 1.747

7.384 ± 0.156

2.889 ± 1.292

4.334 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski