Xylanimonas cellulosilytica (strain DSM 15894 / CECT 5975 / LMG 20990 / XIL07)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Promicromonosporaceae; Xylanimonas; Xylanimonas cellulosilytica

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3441 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D1C0I5|D1C0I5_XYLCX Uncharacterized protein OS=Xylanimonas cellulosilytica (strain DSM 15894 / CECT 5975 / LMG 20990 / XIL07) OX=446471 GN=Xcel_3188 PE=4 SV=1
MM1 pKa = 7.45LGVGDD6 pKa = 5.12ADD8 pKa = 3.76PTKK11 pKa = 9.52EE12 pKa = 3.95PQMTRR17 pKa = 11.84STMRR21 pKa = 11.84PTARR25 pKa = 11.84LSARR29 pKa = 11.84PAARR33 pKa = 11.84LAAAAATATCLVAVAACSGPSDD55 pKa = 4.01AGTSASDD62 pKa = 3.71DD63 pKa = 3.62ATVGPLEE70 pKa = 4.21AMITEE75 pKa = 5.01IVGDD79 pKa = 3.93WNSAEE84 pKa = 4.06QQAKK88 pKa = 8.36QMEE91 pKa = 4.48MEE93 pKa = 4.19EE94 pKa = 4.02AVARR98 pKa = 11.84CMADD102 pKa = 2.64EE103 pKa = 4.5GFEE106 pKa = 4.09YY107 pKa = 10.88TPVDD111 pKa = 3.78YY112 pKa = 10.65SALMAEE118 pKa = 4.51VDD120 pKa = 4.09AVDD123 pKa = 3.51TTTRR127 pKa = 11.84EE128 pKa = 3.86YY129 pKa = 10.65AQEE132 pKa = 3.78YY133 pKa = 9.94GYY135 pKa = 11.12GHH137 pKa = 7.3SIQPDD142 pKa = 3.63TTGNPMPEE150 pKa = 3.52FVDD153 pKa = 3.74PNEE156 pKa = 4.8DD157 pKa = 3.18YY158 pKa = 10.97VAAMSEE164 pKa = 4.55SEE166 pKa = 4.12SEE168 pKa = 4.22AYY170 pKa = 10.57YY171 pKa = 10.22EE172 pKa = 4.06ALYY175 pKa = 10.81GAMEE179 pKa = 4.31YY180 pKa = 10.76DD181 pKa = 3.58VDD183 pKa = 4.16NVDD186 pKa = 5.28SIPDD190 pKa = 3.69MSEE193 pKa = 3.67MGCTGAAQVEE203 pKa = 4.55IMGQQDD209 pKa = 3.03TSLFDD214 pKa = 3.93NEE216 pKa = 4.02EE217 pKa = 3.77LTAFGEE223 pKa = 4.2NMEE226 pKa = 4.12QLEE229 pKa = 4.58ADD231 pKa = 3.71VAADD235 pKa = 3.66PKK237 pKa = 10.65VAEE240 pKa = 4.75AAAGWAEE247 pKa = 4.06CMADD251 pKa = 3.82AGFDD255 pKa = 3.49YY256 pKa = 8.61ATPQEE261 pKa = 4.29AQDD264 pKa = 4.3DD265 pKa = 4.45FMTRR269 pKa = 11.84SQAIWEE275 pKa = 4.26EE276 pKa = 3.94ADD278 pKa = 3.88PEE280 pKa = 4.56SVDD283 pKa = 5.36APEE286 pKa = 4.24QDD288 pKa = 4.28ADD290 pKa = 3.77LQEE293 pKa = 4.32QEE295 pKa = 4.65RR296 pKa = 11.84EE297 pKa = 3.92AAVADD302 pKa = 4.57FDD304 pKa = 4.65CKK306 pKa = 10.99EE307 pKa = 4.0KK308 pKa = 10.99VGYY311 pKa = 9.71DD312 pKa = 3.74AIRR315 pKa = 11.84RR316 pKa = 11.84EE317 pKa = 4.04VQFALEE323 pKa = 3.77QEE325 pKa = 4.67YY326 pKa = 10.41IDD328 pKa = 3.5THH330 pKa = 5.01EE331 pKa = 5.05AEE333 pKa = 4.49LEE335 pKa = 3.99AVKK338 pKa = 10.43AAFEE342 pKa = 4.38SLGLL346 pKa = 3.82

Molecular weight: 37.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D1BW16|D1BW16_XYLCX Proton-translocating NADH-quinone oxidoreductase chain L OS=Xylanimonas cellulosilytica (strain DSM 15894 / CECT 5975 / LMG 20990 / XIL07) OX=446471 GN=Xcel_0480 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3441

0

3441

1156556

30

2724

336.1

35.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.42 ± 0.073

0.537 ± 0.01

6.441 ± 0.034

5.294 ± 0.036

2.654 ± 0.027

9.251 ± 0.031

2.126 ± 0.022

3.246 ± 0.032

1.613 ± 0.028

10.114 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.608 ± 0.019

1.639 ± 0.024

5.955 ± 0.038

2.719 ± 0.022

7.63 ± 0.052

4.843 ± 0.027

6.592 ± 0.04

9.926 ± 0.051

1.591 ± 0.019

1.8 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski