Human associated porprismacovirus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Porprismacovirus

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5GSS8|A0A0B5GSS8_9VIRU Rep protein OS=Human associated porprismacovirus 2 OX=2170114 GN=rep PE=4 SV=1
MM1 pKa = 7.86DD2 pKa = 3.73VVILATNYY10 pKa = 10.61ASASYY15 pKa = 10.76QEE17 pKa = 4.29ILDD20 pKa = 3.88VNTVKK25 pKa = 11.08GNVTVIGIHH34 pKa = 5.93TPTGSTPVRR43 pKa = 11.84RR44 pKa = 11.84LSGFFDD50 pKa = 3.23QFRR53 pKa = 11.84KK54 pKa = 9.61FRR56 pKa = 11.84YY57 pKa = 8.93KK58 pKa = 9.37GCSVQVVPAATLPADD73 pKa = 3.8PLQVGFEE80 pKa = 4.13AGEE83 pKa = 3.95NTIDD87 pKa = 3.9MRR89 pKa = 11.84DD90 pKa = 3.58MLNPILFHH98 pKa = 5.87GTHH101 pKa = 6.44GEE103 pKa = 4.08SLQIAWNNIFVNPNRR118 pKa = 11.84YY119 pKa = 9.87DD120 pKa = 3.43GGADD124 pKa = 3.18TASYY128 pKa = 11.09SSGAGVDD135 pKa = 4.04ASDD138 pKa = 3.5LTFGNVTNSPVEE150 pKa = 3.94TQYY153 pKa = 11.47YY154 pKa = 8.63QAITDD159 pKa = 3.72RR160 pKa = 11.84SWRR163 pKa = 11.84KK164 pKa = 9.57FGIQSTFKK172 pKa = 10.76LPFMSPRR179 pKa = 11.84VWKK182 pKa = 10.6ASTLYY187 pKa = 10.14PIIPNSLQANGSMNTGNTTLPEE209 pKa = 4.29GFLPPGTYY217 pKa = 9.77FDD219 pKa = 4.72NYY221 pKa = 10.42DD222 pKa = 3.45GRR224 pKa = 11.84GLTMPIDD231 pKa = 4.53ADD233 pKa = 3.9QHH235 pKa = 6.91VLDD238 pKa = 4.51QKK240 pKa = 10.39WMSSGTTPLGWLPTVTMPRR259 pKa = 11.84VDD261 pKa = 4.08PNNGTSVVMAPRR273 pKa = 11.84TVLPKK278 pKa = 9.95IFMGILVLPPSYY290 pKa = 10.47LQEE293 pKa = 3.98LYY295 pKa = 10.34FRR297 pKa = 11.84VIITHH302 pKa = 5.5YY303 pKa = 10.91YY304 pKa = 7.78EE305 pKa = 4.68FKK307 pKa = 11.16GFTASLAPASVMPDD321 pKa = 2.78NKK323 pKa = 9.63GTYY326 pKa = 9.35EE327 pKa = 4.05NAVKK331 pKa = 8.75QTGTKK336 pKa = 9.46AASPEE341 pKa = 4.13EE342 pKa = 4.35VEE344 pKa = 4.5DD345 pKa = 3.94TTLEE349 pKa = 4.17SPTANVVRR357 pKa = 11.84ISDD360 pKa = 3.85GVFF363 pKa = 2.82

Molecular weight: 39.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5GSS8|A0A0B5GSS8_9VIRU Rep protein OS=Human associated porprismacovirus 2 OX=2170114 GN=rep PE=4 SV=1
MM1 pKa = 6.35VQAWMITAPRR11 pKa = 11.84ANVSKK16 pKa = 10.49RR17 pKa = 11.84AIRR20 pKa = 11.84IMIDD24 pKa = 3.47KK25 pKa = 10.07NDD27 pKa = 3.64CKK29 pKa = 10.73KK30 pKa = 10.16WIIAKK35 pKa = 10.02EE36 pKa = 3.93KK37 pKa = 10.17GKK39 pKa = 10.3NGYY42 pKa = 7.38EE43 pKa = 3.45HH44 pKa = 5.56WQIRR48 pKa = 11.84IEE50 pKa = 4.12SSNTGFFEE58 pKa = 3.88WCKK61 pKa = 10.68LYY63 pKa = 10.51IPTAHH68 pKa = 6.08VEE70 pKa = 3.9KK71 pKa = 10.78AEE73 pKa = 4.15RR74 pKa = 11.84GVDD77 pKa = 3.37EE78 pKa = 4.63SRR80 pKa = 11.84YY81 pKa = 5.05EE82 pKa = 4.2TKK84 pKa = 9.96EE85 pKa = 3.77GKK87 pKa = 9.88YY88 pKa = 10.37VLYY91 pKa = 9.53TDD93 pKa = 3.42RR94 pKa = 11.84VEE96 pKa = 4.03ILKK99 pKa = 9.93QRR101 pKa = 11.84FGKK104 pKa = 8.87MRR106 pKa = 11.84PNQIRR111 pKa = 11.84ALEE114 pKa = 4.11ALEE117 pKa = 4.1ATNDD121 pKa = 3.59RR122 pKa = 11.84EE123 pKa = 4.21VLVWYY128 pKa = 10.07DD129 pKa = 3.09EE130 pKa = 4.29GGNVGKK136 pKa = 10.39SWLTGALWEE145 pKa = 4.84RR146 pKa = 11.84GLAYY150 pKa = 9.61YY151 pKa = 9.54VPPTVDD157 pKa = 2.8SVKK160 pKa = 11.22AMVQWVASCYY170 pKa = 9.73QSGGWRR176 pKa = 11.84PYY178 pKa = 10.81VIIDD182 pKa = 4.59IPRR185 pKa = 11.84SWKK188 pKa = 9.59WSEE191 pKa = 3.76QLYY194 pKa = 10.02VAIEE198 pKa = 4.26SIKK201 pKa = 11.03DD202 pKa = 3.15GLVYY206 pKa = 9.39DD207 pKa = 4.17TRR209 pKa = 11.84YY210 pKa = 9.55HH211 pKa = 5.6AQCINIRR218 pKa = 11.84GVKK221 pKa = 10.09VLVLTNTQPKK231 pKa = 9.91LDD233 pKa = 4.28KK234 pKa = 10.94LSADD238 pKa = 3.24RR239 pKa = 11.84WRR241 pKa = 11.84ICVFRR246 pKa = 11.84GRR248 pKa = 11.84RR249 pKa = 11.84AAGSALIVTLGADD262 pKa = 4.35FTPLL266 pKa = 3.22

Molecular weight: 30.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

629

266

363

314.5

35.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.154 ± 0.481

0.954 ± 0.602

5.246 ± 0.233

4.769 ± 1.054

3.657 ± 1.155

7.472 ± 0.458

1.272 ± 0.094

6.041 ± 0.96

5.087 ± 1.58

6.836 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.544 ± 0.432

4.769 ± 0.901

5.882 ± 1.624

3.498 ± 0.074

5.564 ± 1.515

6.2 ± 0.853

7.631 ± 1.783

8.267 ± 0.002

2.703 ± 1.175

4.452 ± 0.283
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski