Rummeliibacillus sp. TYF005

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Planococcaceae; Rummeliibacillus; unclassified Rummeliibacillus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3544 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N5ZX21|A0A3N5ZX21_9BACL Stage IV sporulation protein FB OS=Rummeliibacillus sp. TYF005 OX=2058214 GN=CW357_01485 PE=3 SV=1
MM1 pKa = 6.47TQEE4 pKa = 4.44HH5 pKa = 5.69EE6 pKa = 4.65HH7 pKa = 5.25NHH9 pKa = 4.05EE10 pKa = 4.63HH11 pKa = 7.11IIAVDD16 pKa = 3.6DD17 pKa = 4.2EE18 pKa = 4.97GNEE21 pKa = 3.68QVYY24 pKa = 10.38AVLYY28 pKa = 9.89TFDD31 pKa = 4.38SDD33 pKa = 5.45DD34 pKa = 3.61FGKK37 pKa = 10.75SYY39 pKa = 11.1VLYY42 pKa = 8.88YY43 pKa = 10.67KK44 pKa = 10.61EE45 pKa = 4.0GAEE48 pKa = 3.97EE49 pKa = 4.55GEE51 pKa = 4.23EE52 pKa = 4.05VEE54 pKa = 4.94IFASAINPAEE64 pKa = 4.56DD65 pKa = 3.99GEE67 pKa = 4.55GGEE70 pKa = 4.52LVPIEE75 pKa = 4.39TDD77 pKa = 3.56EE78 pKa = 4.05EE79 pKa = 4.31WDD81 pKa = 3.53MVEE84 pKa = 4.56EE85 pKa = 4.24VLNTLDD91 pKa = 4.3AEE93 pKa = 4.54FGEE96 pKa = 4.86EE97 pKa = 3.8

Molecular weight: 11.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N6A8Q3|A0A3N6A8Q3_9BACL UPF0223 protein CW357_16560 OS=Rummeliibacillus sp. TYF005 OX=2058214 GN=CW357_16560 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.54QPKK8 pKa = 9.44KK9 pKa = 7.96RR10 pKa = 11.84KK11 pKa = 8.69HH12 pKa = 5.94SKK14 pKa = 8.54VHH16 pKa = 5.66GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.38NGRR28 pKa = 11.84KK29 pKa = 9.39VIAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.8GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3544

0

3544

1023313

14

1600

288.7

32.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.83 ± 0.047

0.728 ± 0.015

5.149 ± 0.033

7.222 ± 0.046

4.502 ± 0.036

6.417 ± 0.042

2.047 ± 0.019

8.356 ± 0.046

7.643 ± 0.045

9.505 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.67 ± 0.019

4.77 ± 0.037

3.409 ± 0.022

3.864 ± 0.029

3.703 ± 0.033

6.047 ± 0.034

5.673 ± 0.034

6.809 ± 0.037

0.969 ± 0.016

3.689 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski