Red clover powdery mildew-associated totivirus 5

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; unclassified Totiviridae

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0S3Q2D7|A0A0S3Q2D7_9VIRU RNA-directed RNA polymerase OS=Red clover powdery mildew-associated totivirus 5 OX=1714366 GN=RdRp PE=3 SV=1
MM1 pKa = 8.01EE2 pKa = 4.05YY3 pKa = 10.11TRR5 pKa = 11.84GLFNVDD11 pKa = 3.03GMIEE15 pKa = 4.2SFGDD19 pKa = 3.17GLIYY23 pKa = 10.61HH24 pKa = 6.35KK25 pKa = 9.75TRR27 pKa = 11.84LALNIADD34 pKa = 4.02YY35 pKa = 11.25NRR37 pKa = 11.84ADD39 pKa = 4.21FGTGDD44 pKa = 3.13IQMNRR49 pKa = 11.84HH50 pKa = 4.43STVNVRR56 pKa = 11.84SDD58 pKa = 3.34RR59 pKa = 11.84VSALAKK65 pKa = 10.1LSEE68 pKa = 4.51SNGEE72 pKa = 3.81KK73 pKa = 10.42FKK75 pKa = 10.91FRR77 pKa = 11.84RR78 pKa = 11.84LTNCLSQSTVYY89 pKa = 10.74GVVNTLRR96 pKa = 11.84STTPQHH102 pKa = 6.86SYY104 pKa = 11.25LGMSKK109 pKa = 10.43KK110 pKa = 10.63YY111 pKa = 8.9LTNEE115 pKa = 3.92GLPNEE120 pKa = 4.09QIIIKK125 pKa = 9.93RR126 pKa = 11.84LRR128 pKa = 11.84EE129 pKa = 3.97LGMHH133 pKa = 6.8HH134 pKa = 6.95DD135 pKa = 3.78VKK137 pKa = 10.66EE138 pKa = 3.87ARR140 pKa = 11.84FGQFYY145 pKa = 11.56NMLFTKK151 pKa = 10.08DD152 pKa = 3.75YY153 pKa = 8.61YY154 pKa = 11.55DD155 pKa = 3.75NMTALFAKK163 pKa = 10.22LHH165 pKa = 5.84LFSTRR170 pKa = 11.84LSIQEE175 pKa = 3.66RR176 pKa = 11.84MGVHH180 pKa = 6.35DD181 pKa = 4.85HH182 pKa = 7.26DD183 pKa = 4.75VVISTASLTKK193 pKa = 9.97MVGLANDD200 pKa = 4.49DD201 pKa = 3.3EE202 pKa = 4.51RR203 pKa = 11.84LAYY206 pKa = 9.9FKK208 pKa = 11.08SLLSSGTGRR217 pKa = 11.84LGFYY221 pKa = 9.56EE222 pKa = 3.75QDD224 pKa = 3.7YY225 pKa = 10.95NLEE228 pKa = 4.11TMLQNSIAWFNVNKK242 pKa = 9.99NDD244 pKa = 3.83PAARR248 pKa = 11.84EE249 pKa = 3.72YY250 pKa = 10.82RR251 pKa = 11.84QTVKK255 pKa = 9.74VWHH258 pKa = 6.41MYY260 pKa = 10.33KK261 pKa = 10.26YY262 pKa = 10.85DD263 pKa = 4.27DD264 pKa = 3.63GHH266 pKa = 5.97NVSGRR271 pKa = 11.84SFGSEE276 pKa = 3.67FGFDD280 pKa = 3.06GGKK283 pKa = 10.0FLIPEE288 pKa = 4.13CHH290 pKa = 5.22NTINYY295 pKa = 8.89IDD297 pKa = 3.93GVKK300 pKa = 10.0LVKK303 pKa = 10.73SRR305 pKa = 11.84DD306 pKa = 3.02NWFSDD311 pKa = 3.38EE312 pKa = 4.78NIDD315 pKa = 4.79KK316 pKa = 10.79FGSRR320 pKa = 11.84YY321 pKa = 10.12AGYY324 pKa = 10.77LNLSGMTEE332 pKa = 3.95LEE334 pKa = 3.86IAIVNHH340 pKa = 6.3ILLNTDD346 pKa = 2.39RR347 pKa = 11.84MTPFLCDD354 pKa = 3.26QEE356 pKa = 5.39LALCSDD362 pKa = 3.46SDD364 pKa = 3.78KK365 pKa = 11.21FLALASGEE373 pKa = 4.01ISTKK377 pKa = 7.64QTVVSSKK384 pKa = 7.27QVRR387 pKa = 11.84TVMSKK392 pKa = 10.59LAMNHH397 pKa = 6.16RR398 pKa = 11.84AQEE401 pKa = 4.11DD402 pKa = 3.81MLCAVRR408 pKa = 11.84SCKK411 pKa = 10.31YY412 pKa = 10.74LLAQPANEE420 pKa = 4.19TAEE423 pKa = 4.31SHH425 pKa = 4.84WWNVLPKK432 pKa = 10.16IVDD435 pKa = 3.99FPKK438 pKa = 10.2MGLKK442 pKa = 9.97RR443 pKa = 11.84AALHH447 pKa = 6.91FMLEE451 pKa = 4.23DD452 pKa = 3.3EE453 pKa = 4.96GVCISVDD460 pKa = 3.7SIKK463 pKa = 10.67YY464 pKa = 8.8ARR466 pKa = 11.84EE467 pKa = 3.79YY468 pKa = 10.99LSKK471 pKa = 10.5CDD473 pKa = 3.57RR474 pKa = 11.84AILEE478 pKa = 4.48SVFVNSCWFWAEE490 pKa = 3.57YY491 pKa = 8.73LTIYY495 pKa = 10.82NKK497 pKa = 9.87TNSYY501 pKa = 11.16DD502 pKa = 3.38LAAGLLRR509 pKa = 11.84GNDD512 pKa = 2.82SWLVEE517 pKa = 4.09RR518 pKa = 11.84EE519 pKa = 3.99RR520 pKa = 11.84PDD522 pKa = 3.03IMVSALIGRR531 pKa = 11.84PVPIAVHH538 pKa = 5.65SCCTTQWAKK547 pKa = 10.4PLSEE551 pKa = 4.04LARR554 pKa = 11.84LKK556 pKa = 10.92VCFGTISLTGRR567 pKa = 11.84DD568 pKa = 4.01DD569 pKa = 3.99YY570 pKa = 11.88NYY572 pKa = 9.78TLSDD576 pKa = 3.27KK577 pKa = 11.11DD578 pKa = 3.87VIFNSVVPPSGLALVTGLGGSLIQGTPYY606 pKa = 11.21GSMFGINQAVKK617 pKa = 9.5VRR619 pKa = 11.84KK620 pKa = 9.56GMRR623 pKa = 11.84TITSLNYY630 pKa = 9.87NDD632 pKa = 4.27LWALGVVSRR641 pKa = 11.84FNGYY645 pKa = 7.88DD646 pKa = 3.45LKK648 pKa = 11.1YY649 pKa = 10.23KK650 pKa = 10.53CPSSEE655 pKa = 4.11NVHH658 pKa = 6.54TIYY661 pKa = 10.82AANNVSIANPPVLFDD676 pKa = 4.44PEE678 pKa = 4.2ASNLSYY684 pKa = 10.72AIRR687 pKa = 11.84SITEE691 pKa = 3.39RR692 pKa = 11.84DD693 pKa = 3.2IVFGSDD699 pKa = 2.71TTTLLSSKK707 pKa = 10.68SVFYY711 pKa = 10.59WNRR714 pKa = 11.84FEE716 pKa = 4.4STTLEE721 pKa = 4.13SPKK724 pKa = 9.61WSAGIISNDD733 pKa = 3.03VEE735 pKa = 4.23EE736 pKa = 4.58AVLIRR741 pKa = 11.84GFEE744 pKa = 4.26CYY746 pKa = 10.36LDD748 pKa = 3.58EE749 pKa = 4.45TKK751 pKa = 10.54KK752 pKa = 9.27YY753 pKa = 8.19TVAISAEE760 pKa = 4.06YY761 pKa = 10.19DD762 pKa = 3.59VEE764 pKa = 4.47VSDD767 pKa = 4.05FHH769 pKa = 7.0VAKK772 pKa = 9.14LTAGIGLPTLDD783 pKa = 5.05GGLLLDD789 pKa = 4.58AEE791 pKa = 4.69NPAEE795 pKa = 4.14QTTEE799 pKa = 3.49LDD801 pKa = 3.62MPVLEE806 pKa = 5.46DD807 pKa = 3.4SALGAGSS814 pKa = 3.25

Molecular weight:
91.33 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0S3Q2D7|A0A0S3Q2D7_9VIRU RNA-directed RNA polymerase OS=Red clover powdery mildew-associated totivirus 5 OX=1714366 GN=RdRp PE=3 SV=1
MM1 pKa = 7.81PYY3 pKa = 10.36VPTFLAPTDD12 pKa = 3.45SSRR15 pKa = 11.84VFRR18 pKa = 11.84EE19 pKa = 3.36VDD21 pKa = 3.86FNEE24 pKa = 3.99ADD26 pKa = 3.56LVLFEE31 pKa = 4.96CLSGLEE37 pKa = 4.71LDD39 pKa = 4.77GSMHH43 pKa = 6.96LHH45 pKa = 5.45IQEE48 pKa = 4.13SAVPIIAVYY57 pKa = 9.82LPKK60 pKa = 10.83VKK62 pKa = 9.28VTACYY67 pKa = 10.25ISIDD71 pKa = 3.77YY72 pKa = 9.9QIKK75 pKa = 8.36HH76 pKa = 5.27TNKK79 pKa = 10.16DD80 pKa = 3.08VLLRR84 pKa = 11.84FSRR87 pKa = 11.84MQYY90 pKa = 10.96GPDD93 pKa = 3.66LFPFGLVSDD102 pKa = 4.65LDD104 pKa = 3.46IVRR107 pKa = 11.84YY108 pKa = 10.13AFFISRR114 pKa = 11.84KK115 pKa = 6.91TIKK118 pKa = 9.83KK119 pKa = 10.01RR120 pKa = 11.84KK121 pKa = 8.96DD122 pKa = 3.3YY123 pKa = 10.45KK124 pKa = 10.86IKK126 pKa = 10.49DD127 pKa = 3.62FPIIDD132 pKa = 3.24SWFRR136 pKa = 11.84GSTEE140 pKa = 3.87PPVSKK145 pKa = 10.84VSVNHH150 pKa = 5.79LRR152 pKa = 11.84HH153 pKa = 4.7MTMAEE158 pKa = 3.78VRR160 pKa = 11.84LMGRR164 pKa = 11.84GVIEE168 pKa = 4.86DD169 pKa = 3.54KK170 pKa = 11.07VSYY173 pKa = 10.29ILPFLSRR180 pKa = 11.84MAKK183 pKa = 10.0VGMSEE188 pKa = 3.94AMFIGIVLWANSLPTQQHH206 pKa = 5.58EE207 pKa = 4.29LCKK210 pKa = 10.82LSGLWDD216 pKa = 3.9LSFTSMEE223 pKa = 4.16DD224 pKa = 3.03FATKK228 pKa = 10.24VKK230 pKa = 10.81ARR232 pKa = 11.84FSLRR236 pKa = 11.84LKK238 pKa = 10.48ALQNLVSVDD247 pKa = 3.22LHH249 pKa = 6.71CFFEE253 pKa = 5.57LEE255 pKa = 4.2VLVNRR260 pKa = 11.84GVGEE264 pKa = 4.23LNWEE268 pKa = 4.03KK269 pKa = 11.16EE270 pKa = 3.95KK271 pKa = 10.86MNRR274 pKa = 11.84TRR276 pKa = 11.84PNLAQFKK283 pKa = 9.32PEE285 pKa = 3.89QVFSKK290 pKa = 10.53ACSLFTDD297 pKa = 3.77ILRR300 pKa = 11.84RR301 pKa = 11.84GGRR304 pKa = 11.84PKK306 pKa = 10.2KK307 pKa = 10.52RR308 pKa = 11.84NWSEE312 pKa = 3.53FWSNRR317 pKa = 11.84WEE319 pKa = 3.98WAPTGSSHH327 pKa = 6.18SQYY330 pKa = 11.54AEE332 pKa = 3.9DD333 pKa = 5.31DD334 pKa = 4.1VYY336 pKa = 11.44KK337 pKa = 11.07SKK339 pKa = 11.49DD340 pKa = 3.05PFNRR344 pKa = 11.84HH345 pKa = 5.63KK346 pKa = 11.06LFTLCSMPEE355 pKa = 3.47RR356 pKa = 11.84SFEE359 pKa = 4.1YY360 pKa = 9.93FLKK363 pKa = 10.63RR364 pKa = 11.84KK365 pKa = 9.47PEE367 pKa = 4.33TIAWTSTKK375 pKa = 10.56YY376 pKa = 10.29EE377 pKa = 4.04WTKK380 pKa = 9.96MRR382 pKa = 11.84AIYY385 pKa = 10.14GVDD388 pKa = 2.52ITNFIMTTFAMGDD401 pKa = 3.84CEE403 pKa = 5.59DD404 pKa = 3.88VLSSKK409 pKa = 10.51FPIGKK414 pKa = 9.2AANEE418 pKa = 4.11VNVRR422 pKa = 11.84STVKK426 pKa = 9.39TLLGNGVPFCFDD438 pKa = 3.37FEE440 pKa = 5.02DD441 pKa = 4.71FNSQHH446 pKa = 6.7SIEE449 pKa = 5.01SMVQVIRR456 pKa = 11.84AYY458 pKa = 10.32SSTFKK463 pKa = 10.07MHH465 pKa = 6.86MSEE468 pKa = 3.98QQYY471 pKa = 10.66NAIDD475 pKa = 3.5WVCRR479 pKa = 11.84SFYY482 pKa = 11.04DD483 pKa = 3.83SFVVEE488 pKa = 5.0DD489 pKa = 3.57NRR491 pKa = 11.84GDD493 pKa = 3.79KK494 pKa = 11.01DD495 pKa = 3.47KK496 pKa = 11.52GPCNSSASSRR506 pKa = 11.84EE507 pKa = 3.5RR508 pKa = 11.84AKK510 pKa = 10.11MYY512 pKa = 9.74RR513 pKa = 11.84TMGTLLSGCRR523 pKa = 11.84LTTFLNTVLNYY534 pKa = 9.92IYY536 pKa = 11.09VNLSIEE542 pKa = 4.26GDD544 pKa = 3.82DD545 pKa = 6.35LIATHH550 pKa = 6.59NGDD553 pKa = 4.92DD554 pKa = 3.38ILAVVSSYY562 pKa = 11.45RR563 pKa = 11.84EE564 pKa = 3.63VQAISRR570 pKa = 11.84GAEE573 pKa = 3.38RR574 pKa = 11.84HH575 pKa = 5.2QIRR578 pKa = 11.84FQPSKK583 pKa = 10.88CFLGSVAEE591 pKa = 4.17FLRR594 pKa = 11.84IDD596 pKa = 3.48HH597 pKa = 6.79HH598 pKa = 7.95SGAGGQYY605 pKa = 8.12LTRR608 pKa = 11.84AISTLVHH615 pKa = 6.77GPTEE619 pKa = 3.89MAVPNNLVAQLTAINTRR636 pKa = 11.84ANEE639 pKa = 3.89AKK641 pKa = 10.64ARR643 pKa = 11.84GADD646 pKa = 3.23HH647 pKa = 7.03AFIAGVVKK655 pKa = 10.67SQLPYY660 pKa = 10.42LCKK663 pKa = 9.99VWQITEE669 pKa = 4.32DD670 pKa = 3.42EE671 pKa = 4.49VEE673 pKa = 4.48AVFSTHH679 pKa = 6.21LCFGGLSNEE688 pKa = 4.04ITPKK692 pKa = 10.49SLSKK696 pKa = 10.83SIILEE701 pKa = 3.83EE702 pKa = 5.74DD703 pKa = 2.42ITANKK708 pKa = 9.37RR709 pKa = 11.84PSVLADD715 pKa = 3.21EE716 pKa = 5.73GRR718 pKa = 11.84FFPGAFSYY726 pKa = 10.87AKK728 pKa = 10.04SLTSTIIPEE737 pKa = 4.21SYY739 pKa = 9.77FGKK742 pKa = 10.29VEE744 pKa = 4.04HH745 pKa = 6.39AVSRR749 pKa = 11.84TVTALSCSRR758 pKa = 11.84RR759 pKa = 11.84FRR761 pKa = 11.84LVVKK765 pKa = 10.24DD766 pKa = 3.62NKK768 pKa = 10.14TPGVTDD774 pKa = 3.59YY775 pKa = 11.23IKK777 pKa = 10.9ANRR780 pKa = 11.84YY781 pKa = 9.17GLFRR785 pKa = 11.84SKK787 pKa = 9.69LTAGKK792 pKa = 8.27ITLARR797 pKa = 11.84AFNIPIIDD805 pKa = 3.3IKK807 pKa = 9.71YY808 pKa = 8.46QKK810 pKa = 10.34QEE812 pKa = 3.69ILEE815 pKa = 4.21ILASEE820 pKa = 4.05QDD822 pKa = 3.12KK823 pKa = 10.93MEE825 pKa = 4.56ALRR828 pKa = 11.84ILFF831 pKa = 4.11

Molecular weight:
94.54 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1645

814

831

822.5

92.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.626 ± 0.006

1.763 ± 0.034

5.714 ± 0.439

5.836 ± 0.147

5.228 ± 0.739

5.836 ± 0.635

2.249 ± 0.03

5.593 ± 0.443

5.836 ± 0.538

9.422 ± 0.714

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.675 ± 0.022

4.863 ± 0.725

3.343 ± 0.412

2.492 ± 0.224

5.653 ± 0.491

8.754 ± 0.025

5.897 ± 0.196

6.93 ± 0.138

1.459 ± 0.012

3.83 ± 0.472

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski