Pseudoalteromonas phage SL20

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae; Pseudoalteromonas virus J2-1

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A223LH43|A0A223LH43_9CAUD Exodeoxyribonuclease X OS=Pseudoalteromonas phage SL20 OX=2023998 PE=4 SV=1
MM1 pKa = 7.24NKK3 pKa = 9.58IYY5 pKa = 10.85NLTEE9 pKa = 4.33GDD11 pKa = 3.37WTNASEE17 pKa = 4.35DD18 pKa = 3.62VNEE21 pKa = 4.15IVYY24 pKa = 10.19QYY26 pKa = 11.27LEE28 pKa = 4.34NYY30 pKa = 9.03PKK32 pKa = 10.86EE33 pKa = 3.96EE34 pKa = 4.67LIATKK39 pKa = 10.48SVFLYY44 pKa = 10.65EE45 pKa = 4.16GVPVQKK51 pKa = 10.95YY52 pKa = 7.28MSDD55 pKa = 4.01YY56 pKa = 11.01IDD58 pKa = 3.12VDD60 pKa = 4.15YY61 pKa = 10.96ILDD64 pKa = 3.68MAEE67 pKa = 4.66SNACDD72 pKa = 3.36EE73 pKa = 4.39VGEE76 pKa = 4.31FAEE79 pKa = 4.91GWLDD83 pKa = 4.17YY84 pKa = 10.37VTTPQRR90 pKa = 11.84DD91 pKa = 3.51EE92 pKa = 4.01LAEE95 pKa = 4.49MITLWANKK103 pKa = 9.12NHH105 pKa = 6.29LQPKK109 pKa = 7.94FQHH112 pKa = 5.45VDD114 pKa = 3.16EE115 pKa = 4.76VEE117 pKa = 4.29EE118 pKa = 4.42IEE120 pKa = 4.62VEE122 pKa = 3.62VDD124 pKa = 3.46EE125 pKa = 5.61IIRR128 pKa = 11.84NEE130 pKa = 4.02LLKK133 pKa = 11.02LLEE136 pKa = 4.63GEE138 pKa = 4.34

Molecular weight: 16.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A223LIP5|A0A223LIP5_9CAUD Putative polynucleotide 5'-kinase and 3'-phosphatase OS=Pseudoalteromonas phage SL20 OX=2023998 PE=4 SV=1
MM1 pKa = 7.18TRR3 pKa = 11.84INTIDD8 pKa = 3.99PSDD11 pKa = 4.56LTDD14 pKa = 2.82QWLIAEE20 pKa = 4.16WRR22 pKa = 11.84EE23 pKa = 3.56LPRR26 pKa = 11.84IVNEE30 pKa = 4.36LVTHH34 pKa = 5.49PHH36 pKa = 6.62RR37 pKa = 11.84FKK39 pKa = 11.22QKK41 pKa = 10.39DD42 pKa = 2.97IPANYY47 pKa = 8.48TLNAGHH53 pKa = 6.72VKK55 pKa = 10.24FFRR58 pKa = 11.84NKK60 pKa = 10.17LLYY63 pKa = 9.32LAKK66 pKa = 10.19RR67 pKa = 11.84HH68 pKa = 5.88RR69 pKa = 11.84LLKK72 pKa = 10.49RR73 pKa = 11.84EE74 pKa = 4.12LKK76 pKa = 10.78ARR78 pKa = 11.84GIKK81 pKa = 9.45HH82 pKa = 6.3DD83 pKa = 4.07KK84 pKa = 8.83NVKK87 pKa = 9.42VEE89 pKa = 4.21LYY91 pKa = 10.8KK92 pKa = 10.46LDD94 pKa = 3.96KK95 pKa = 10.69SIKK98 pKa = 9.97DD99 pKa = 3.42LCCNDD104 pKa = 3.36WTPTPTDD111 pKa = 3.17HH112 pKa = 7.56ALLIEE117 pKa = 4.39RR118 pKa = 11.84LQEE121 pKa = 3.87RR122 pKa = 11.84FNLRR126 pKa = 11.84KK127 pKa = 9.32KK128 pKa = 10.56DD129 pKa = 3.68YY130 pKa = 10.15TLTTVIKK137 pKa = 10.21YY138 pKa = 9.34PNIRR142 pKa = 11.84NSVFKK147 pKa = 11.07NVIDD151 pKa = 5.85DD152 pKa = 3.63DD153 pKa = 4.9HH154 pKa = 7.85SWQIYY159 pKa = 5.44STRR162 pKa = 11.84FKK164 pKa = 10.9KK165 pKa = 10.35YY166 pKa = 10.54LKK168 pKa = 10.59

Molecular weight: 20.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

24286

44

847

255.6

28.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.674 ± 0.273

1.573 ± 0.129

6.753 ± 0.15

7.366 ± 0.256

4.278 ± 0.151

6.786 ± 0.263

1.766 ± 0.1

5.979 ± 0.191

8.095 ± 0.434

8.124 ± 0.209

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.248 ± 0.119

5.715 ± 0.193

3.121 ± 0.132

3.088 ± 0.14

3.883 ± 0.172

7.095 ± 0.24

5.954 ± 0.226

6.662 ± 0.189

1.289 ± 0.076

4.55 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski