Methylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Beijerinckiaceae; Methylocella; Methylocella silvestris

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3816 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B8EQ36|B8EQ36_METSB ABC transporter related OS=Methylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2) OX=395965 GN=Msil_2603 PE=3 SV=1
MM1 pKa = 7.64SGITFIVEE9 pKa = 4.07GATGLDD15 pKa = 3.46EE16 pKa = 4.89QDD18 pKa = 3.39VKK20 pKa = 11.58NSFEE24 pKa = 4.12NLRR27 pKa = 11.84NASGDD32 pKa = 3.37FDD34 pKa = 5.06QDD36 pKa = 2.84ILYY39 pKa = 9.77IASKK43 pKa = 9.3TRR45 pKa = 11.84VLIIIANDD53 pKa = 3.8TVSGTSATYY62 pKa = 9.73PVTSGDD68 pKa = 3.03TAYY71 pKa = 10.96YY72 pKa = 8.45GTYY75 pKa = 9.89DD76 pKa = 3.53YY77 pKa = 11.08VIRR80 pKa = 11.84IPKK83 pKa = 9.84QGFDD87 pKa = 3.3GDD89 pKa = 4.4TYY91 pKa = 11.64NHH93 pKa = 6.52VGTDD97 pKa = 2.81KK98 pKa = 10.76WSLEE102 pKa = 3.53RR103 pKa = 11.84LYY105 pKa = 10.93AHH107 pKa = 6.94EE108 pKa = 4.46VAHH111 pKa = 5.9VMQAIGDD118 pKa = 4.12NAPSTQAEE126 pKa = 4.28RR127 pKa = 11.84EE128 pKa = 4.13EE129 pKa = 4.2QATKK133 pKa = 10.67YY134 pKa = 10.17SNHH137 pKa = 6.11IMSTAHH143 pKa = 6.88PEE145 pKa = 4.03EE146 pKa = 4.88SPQTHH151 pKa = 6.47YY152 pKa = 11.64YY153 pKa = 8.02NTEE156 pKa = 3.76GDD158 pKa = 3.58TGSGLNQNSGSGDD171 pKa = 3.62SPHH174 pKa = 7.97ILDD177 pKa = 4.81DD178 pKa = 4.3SSPLSEE184 pKa = 4.67NLPGAPLPSQLAAIQPAFGDD204 pKa = 4.7AINASSPLVIDD215 pKa = 4.66LSSGHH220 pKa = 6.31SGVTLTTWSASTTEE234 pKa = 4.41TYY236 pKa = 10.87FDD238 pKa = 4.88LDD240 pKa = 4.05DD241 pKa = 4.68NGFAVQTAWVSGDD254 pKa = 3.18TGLLARR260 pKa = 11.84DD261 pKa = 3.68LNEE264 pKa = 3.99NGIIDD269 pKa = 3.83SSAEE273 pKa = 3.95LFGSPTVDD281 pKa = 3.15GFAKK285 pKa = 10.62LAMLDD290 pKa = 3.84SNHH293 pKa = 7.22DD294 pKa = 3.74LRR296 pKa = 11.84IDD298 pKa = 3.96SNDD301 pKa = 3.78DD302 pKa = 2.98AWDD305 pKa = 3.8SLVVWNDD312 pKa = 3.23DD313 pKa = 3.24GDD315 pKa = 4.47AVTQSGEE322 pKa = 4.14LHH324 pKa = 6.37SLASLNIANIDD335 pKa = 3.77LAGVASSTSTISGNSISHH353 pKa = 5.23VSKK356 pKa = 9.69ITFTSGATAAIDD368 pKa = 3.8DD369 pKa = 3.91AWFVHH374 pKa = 7.23DD375 pKa = 4.67NTNSFYY381 pKa = 10.45TGEE384 pKa = 4.13YY385 pKa = 9.05TLDD388 pKa = 3.34VDD390 pKa = 4.06TLFLPRR396 pKa = 11.84VRR398 pKa = 11.84GYY400 pKa = 8.97GTLPDD405 pKa = 3.66LTIAMSLDD413 pKa = 3.57SDD415 pKa = 4.81LKK417 pKa = 11.3DD418 pKa = 3.57LVLGFAGTDD427 pKa = 3.22LSEE430 pKa = 4.23FADD433 pKa = 3.83AKK435 pKa = 10.07TAITDD440 pKa = 4.07ILYY443 pKa = 9.87KK444 pKa = 8.91WAGVEE449 pKa = 4.67GVDD452 pKa = 4.12PDD454 pKa = 4.63SRR456 pKa = 11.84GIWVDD461 pKa = 3.34AQHH464 pKa = 7.13LAFLEE469 pKa = 4.06QFIGSDD475 pKa = 3.48FLQITTHH482 pKa = 6.51SADD485 pKa = 3.58PQPWAGALIEE495 pKa = 4.81DD496 pKa = 5.12AYY498 pKa = 10.94QAAFAMLSADD508 pKa = 3.36ILVQTGFGQLFDD520 pKa = 3.55TAITYY525 pKa = 9.75NPSSGTRR532 pKa = 11.84TGDD535 pKa = 3.14TDD537 pKa = 3.93LSEE540 pKa = 5.05DD541 pKa = 4.59AIDD544 pKa = 4.56DD545 pKa = 4.15LVSIAPSSGPEE556 pKa = 3.71NEE558 pKa = 4.44AFWVSVAWALDD569 pKa = 3.52VTKK572 pKa = 10.81GISNLSYY579 pKa = 11.42DD580 pKa = 3.69EE581 pKa = 4.38TTWLSDD587 pKa = 4.79AITASNALSDD597 pKa = 3.8WATVLHH603 pKa = 6.74SYY605 pKa = 10.64DD606 pKa = 3.81PSAGTASQNGTNGAEE621 pKa = 4.06TLYY624 pKa = 11.42GDD626 pKa = 3.57VTADD630 pKa = 3.87TIHH633 pKa = 6.6GFNGADD639 pKa = 3.82TIYY642 pKa = 11.06GDD644 pKa = 3.8YY645 pKa = 11.29GNDD648 pKa = 2.93ILYY651 pKa = 10.75GDD653 pKa = 5.23DD654 pKa = 4.18GNDD657 pKa = 3.12VIYY660 pKa = 10.63GDD662 pKa = 4.01EE663 pKa = 4.47GADD666 pKa = 3.46VLYY669 pKa = 11.06GGAGNDD675 pKa = 3.51HH676 pKa = 7.3LYY678 pKa = 11.21GGDD681 pKa = 3.79GNDD684 pKa = 4.35TIHH687 pKa = 7.18GEE689 pKa = 4.2YY690 pKa = 11.13GNDD693 pKa = 3.64TLDD696 pKa = 3.84GGAGGNFLSALTGDD710 pKa = 3.62DD711 pKa = 3.39TFVYY715 pKa = 10.47GGGHH719 pKa = 6.38DD720 pKa = 4.25VISDD724 pKa = 3.58TGGSDD729 pKa = 4.02QLLLPSGIVLGDD741 pKa = 3.28LTFQRR746 pKa = 11.84VSTEE750 pKa = 3.67GSLSYY755 pKa = 10.93FDD757 pKa = 5.75DD758 pKa = 4.21LLINIDD764 pKa = 3.53GAGTIEE770 pKa = 4.3IKK772 pKa = 10.84AHH774 pKa = 5.33FQSSSFRR781 pKa = 11.84IEE783 pKa = 3.73TLVFSDD789 pKa = 4.32TSTLDD794 pKa = 3.21LTTLANPDD802 pKa = 3.31VYY804 pKa = 10.47LTAGNDD810 pKa = 3.53TFTSGATGAFAIYY823 pKa = 10.28GGDD826 pKa = 3.43GDD828 pKa = 6.13DD829 pKa = 5.15YY830 pKa = 11.43MSDD833 pKa = 3.86YY834 pKa = 11.39QNAAHH839 pKa = 6.51TFDD842 pKa = 4.47GGNGNDD848 pKa = 3.52AMYY851 pKa = 10.76GGAGNDD857 pKa = 3.48TYY859 pKa = 10.91IASTGFDD866 pKa = 3.91TISEE870 pKa = 4.25NAGTDD875 pKa = 3.83TIVVPAAFTIDD886 pKa = 4.02DD887 pKa = 3.7VTFYY891 pKa = 10.99RR892 pKa = 11.84ILNGSGPTDD901 pKa = 3.31NLGISINGLGEE912 pKa = 3.82IAVIAQFSSALVEE925 pKa = 4.32YY926 pKa = 9.8MHH928 pKa = 7.26FDD930 pKa = 3.48NDD932 pKa = 3.05NSTISLSGLSITTVGTAGNDD952 pKa = 3.45NLSPPSTHH960 pKa = 7.06AGANDD965 pKa = 3.29ILDD968 pKa = 3.73GRR970 pKa = 11.84EE971 pKa = 3.41GDD973 pKa = 4.18DD974 pKa = 3.65YY975 pKa = 12.04VNGGSGDD982 pKa = 3.52DD983 pKa = 3.68TYY985 pKa = 11.31IFSAGHH991 pKa = 5.14DD992 pKa = 4.11TITEE996 pKa = 4.04SSGDD1000 pKa = 3.47DD1001 pKa = 3.69TILVRR1006 pKa = 11.84SSYY1009 pKa = 11.47APGDD1013 pKa = 3.38ISISFVPYY1021 pKa = 10.49YY1022 pKa = 10.94YY1023 pKa = 9.57DD1024 pKa = 3.28TTGMRR1029 pKa = 11.84LEE1031 pKa = 4.51DD1032 pKa = 3.6TDD1034 pKa = 4.19GNSITVYY1041 pKa = 9.82RR1042 pKa = 11.84QGYY1045 pKa = 7.37SSGYY1049 pKa = 8.35VVEE1052 pKa = 5.12HH1053 pKa = 6.15VAFADD1058 pKa = 3.7TTVWNLSSMEE1068 pKa = 4.1IEE1070 pKa = 4.15THH1072 pKa = 5.19GTSSADD1078 pKa = 3.18YY1079 pKa = 10.63LYY1081 pKa = 11.48GHH1083 pKa = 7.34DD1084 pKa = 4.47VGDD1087 pKa = 4.07ASSADD1092 pKa = 3.8TIYY1095 pKa = 10.87GYY1097 pKa = 11.05AGNDD1101 pKa = 3.63TIDD1104 pKa = 3.88AGDD1107 pKa = 4.11GNDD1110 pKa = 4.18LVYY1113 pKa = 11.02AGDD1116 pKa = 3.84GADD1119 pKa = 3.77YY1120 pKa = 11.05VYY1122 pKa = 11.2ASNGNDD1128 pKa = 2.99TVYY1131 pKa = 11.03GGNDD1135 pKa = 3.22ADD1137 pKa = 3.83EE1138 pKa = 4.44LHH1140 pKa = 6.79GKK1142 pKa = 8.37FHH1144 pKa = 6.58SVLYY1148 pKa = 10.94GEE1150 pKa = 5.33AGNDD1154 pKa = 3.27DD1155 pKa = 5.62LYY1157 pKa = 11.73ANPYY1161 pKa = 9.4SNEE1164 pKa = 3.8AAGTLVTLFGGDD1176 pKa = 3.65GADD1179 pKa = 3.45TLHH1182 pKa = 6.51GGNYY1186 pKa = 10.12GEE1188 pKa = 4.53TVMHH1192 pKa = 7.03GGNGVDD1198 pKa = 3.78TMISAYY1204 pKa = 9.35GTDD1207 pKa = 3.31TFAFDD1212 pKa = 3.64AASAFNAVDD1221 pKa = 4.37VIQQFNTSEE1230 pKa = 3.98HH1231 pKa = 6.52DD1232 pKa = 4.06KK1233 pKa = 11.03IDD1235 pKa = 3.42ISDD1238 pKa = 3.97VLDD1241 pKa = 3.47GHH1243 pKa = 6.73YY1244 pKa = 10.96DD1245 pKa = 3.66PLTDD1249 pKa = 5.8AITDD1253 pKa = 4.12FVQITTSGSNSEE1265 pKa = 4.71LFVDD1269 pKa = 4.23TTGSATFGSSQHH1281 pKa = 6.25IATIQYY1287 pKa = 8.05ITGLTDD1293 pKa = 3.17EE1294 pKa = 5.05AALVTAGTLIAAA1306 pKa = 5.1

Molecular weight: 137.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B8EPP1|B8EPP1_METSB Multi_ubiq domain-containing protein OS=Methylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2) OX=395965 GN=Msil_1952 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84RR29 pKa = 11.84IIAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3816

0

3816

1219436

33

2684

319.6

34.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.821 ± 0.064

0.893 ± 0.013

5.541 ± 0.032

5.588 ± 0.04

4.015 ± 0.028

8.475 ± 0.037

1.888 ± 0.018

5.274 ± 0.028

3.469 ± 0.032

10.231 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.097 ± 0.017

2.549 ± 0.024

5.481 ± 0.032

3.014 ± 0.021

7.09 ± 0.043

5.636 ± 0.03

4.716 ± 0.027

6.832 ± 0.034

1.222 ± 0.016

2.169 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski