Trypanosoma theileri

Taxonomy: cellular organisms; Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina; Trypanosomatida; Trypanosomatidae; Trypanosoma; Trypanosoma with unspecified subgenus

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11236 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X0NEG5|A0A1X0NEG5_9TRYP Uncharacterized protein (Fragment) OS=Trypanosoma theileri OX=67003 GN=TM35_000911050 PE=4 SV=1
II1 pKa = 7.41ASQVNRR7 pKa = 11.84IAFCFSEE14 pKa = 5.3GIPALGGSLIGKK26 pKa = 9.23DD27 pKa = 3.64SWCLDD32 pKa = 3.4GEE34 pKa = 4.46SLHH37 pKa = 7.18VNLDD41 pKa = 4.0DD42 pKa = 4.17VNVRR46 pKa = 11.84YY47 pKa = 9.63NVAAVCALVSCDD59 pKa = 3.17EE60 pKa = 4.16SSRR63 pKa = 11.84TVKK66 pKa = 9.93VQYY69 pKa = 10.25NGSDD73 pKa = 3.04EE74 pKa = 4.04WHH76 pKa = 6.48NCPEE80 pKa = 4.4GEE82 pKa = 4.45SIEE85 pKa = 4.52AKK87 pKa = 10.43PPTFKK92 pKa = 10.52SGHH95 pKa = 5.64IKK97 pKa = 10.25CPNYY101 pKa = 10.61TEE103 pKa = 4.27VCTIASNGSSLITVVDD119 pKa = 4.41PPTDD123 pKa = 3.65PTPPPDD129 pKa = 4.0GDD131 pKa = 3.72GGGGGDD137 pKa = 4.92NDD139 pKa = 4.27GDD141 pKa = 4.23DD142 pKa = 3.96AGGDD146 pKa = 3.6GGGGGDD152 pKa = 4.97NVGDD156 pKa = 4.21DD157 pKa = 3.71NAGGGGGGGDD167 pKa = 3.94NVGDD171 pKa = 4.54DD172 pKa = 4.52NAGDD176 pKa = 4.37DD177 pKa = 4.44DD178 pKa = 4.27NAGGGGGGGGHH189 pKa = 7.32GDD191 pKa = 3.5GCATASPIALSALALMVITPMLLPP215 pKa = 4.52

Molecular weight: 21.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X0PA60|A0A1X0PA60_9TRYP N2227 domain-containing protein OS=Trypanosoma theileri OX=67003 GN=TM35_000013900 PE=4 SV=1
QQ1 pKa = 7.92VGVWHH6 pKa = 6.06QNPLALVAVQHH17 pKa = 5.5QQQQQISQQHH27 pKa = 5.09HH28 pKa = 5.93QIPSALAAVLRR39 pKa = 11.84QQQLQINQRR48 pKa = 11.84HH49 pKa = 4.92HH50 pKa = 6.6QIPSALVVVLRR61 pKa = 11.84QQQQINQQHH70 pKa = 5.56HH71 pKa = 6.18QIPSALVVVLRR82 pKa = 11.84QQQLQISLWHH92 pKa = 5.89HH93 pKa = 6.5QIPSALVTVLRR104 pKa = 11.84QQQLQISLRR113 pKa = 11.84RR114 pKa = 11.84HH115 pKa = 5.72QIPSALVXXXXQVGVWHH132 pKa = 6.12QNPSALVVVQHH143 pKa = 5.82QQQQQINQRR152 pKa = 11.84HH153 pKa = 4.8HH154 pKa = 6.47QIPSALAAVLRR165 pKa = 11.84QQQLQINQRR174 pKa = 11.84HH175 pKa = 4.92HH176 pKa = 6.6QIPSALVVVLRR187 pKa = 11.84QQQQINQQHH196 pKa = 5.56HH197 pKa = 6.18QIPSALVVVLRR208 pKa = 11.84QQQLQISLWHH218 pKa = 5.89HH219 pKa = 6.5QIPSALVTVLRR230 pKa = 11.84QQQLQISLRR239 pKa = 11.84RR240 pKa = 11.84HH241 pKa = 5.72QIPSALVTVLHH252 pKa = 5.38QQQLQISLWRR262 pKa = 11.84HH263 pKa = 5.32QIPPALVTVLRR274 pKa = 11.84QQQQISLRR282 pKa = 11.84HH283 pKa = 5.42HH284 pKa = 5.24QTPSVLVTVLHH295 pKa = 6.75RR296 pKa = 11.84VRR298 pKa = 4.97

Molecular weight: 34.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11236

0

11236

5415872

68

6639

482.0

53.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.216 ± 0.024

1.795 ± 0.01

4.899 ± 0.016

7.354 ± 0.029

3.381 ± 0.017

5.751 ± 0.019

2.536 ± 0.011

4.511 ± 0.017

4.979 ± 0.021

9.15 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.42 ± 0.008

5.143 ± 0.026

4.855 ± 0.02

4.192 ± 0.02

6.337 ± 0.019

8.415 ± 0.028

6.609 ± 0.023

6.856 ± 0.018

1.053 ± 0.006

2.542 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski