Pea early browning virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Tobravirus

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P16281|V23K_PEBV 23 kDa protein OS=Pea early browning virus OX=12294 PE=4 SV=1
MM1 pKa = 7.27TNWATLWPNDD11 pKa = 3.99RR12 pKa = 11.84LFLSDD17 pKa = 4.05TYY19 pKa = 11.23QLIWFDD25 pKa = 3.56IEE27 pKa = 4.08ADD29 pKa = 4.3RR30 pKa = 11.84IEE32 pKa = 4.68HH33 pKa = 6.07KK34 pKa = 10.33HH35 pKa = 6.1FKK37 pKa = 9.77AQNSEE42 pKa = 4.6DD43 pKa = 3.1ISMIPKK49 pKa = 10.18GFVSFVDD56 pKa = 3.71NRR58 pKa = 11.84LPMCINHH65 pKa = 6.62KK66 pKa = 9.8GEE68 pKa = 3.83VYY70 pKa = 10.31IRR72 pKa = 11.84VGSFDD77 pKa = 2.96TAYY80 pKa = 9.54YY81 pKa = 10.57QKK83 pKa = 10.86FGDD86 pKa = 4.27LDD88 pKa = 3.64VSDD91 pKa = 6.1FDD93 pKa = 5.38DD94 pKa = 4.18QVLPPDD100 pKa = 4.43RR101 pKa = 11.84DD102 pKa = 3.59FTFNKK107 pKa = 9.65VVFGDD112 pKa = 3.9VPQEE116 pKa = 3.82QLDD119 pKa = 3.76NQIRR123 pKa = 11.84DD124 pKa = 3.73LQSEE128 pKa = 4.44VSILTSRR135 pKa = 11.84NVEE138 pKa = 3.94MNVRR142 pKa = 11.84EE143 pKa = 3.98NDD145 pKa = 3.5LLKK148 pKa = 10.7KK149 pKa = 10.63VSEE152 pKa = 4.23LEE154 pKa = 3.88KK155 pKa = 10.58QIRR158 pKa = 11.84QSSHH162 pKa = 6.13NYY164 pKa = 8.54EE165 pKa = 4.17KK166 pKa = 10.81VVEE169 pKa = 4.57DD170 pKa = 4.3GVVLSYY176 pKa = 11.0RR177 pKa = 11.84KK178 pKa = 10.13AGGLLNRR185 pKa = 11.84MVVLNRR191 pKa = 11.84RR192 pKa = 11.84LVGQRR197 pKa = 11.84FVTNQRR203 pKa = 11.84RR204 pKa = 11.84WEE206 pKa = 4.11NIIMGSGVHH215 pKa = 6.98DD216 pKa = 4.61GSSYY220 pKa = 9.83MAFNFKK226 pKa = 10.58EE227 pKa = 4.28SGGSLKK233 pKa = 9.57VTFDD237 pKa = 3.41FDD239 pKa = 3.66KK240 pKa = 11.1LQNLSPDD247 pKa = 4.27DD248 pKa = 4.2LLAMQIAA255 pKa = 4.0

Molecular weight: 29.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q84703|Q84703_PEBV (PEBV) genomic RNA1 for 141K 201K 30K and 12K proteins OS=Pea early browning virus OX=12294 PE=4 SV=1
MM1 pKa = 7.97DD2 pKa = 6.22IIRR5 pKa = 11.84AADD8 pKa = 3.5VKK10 pKa = 10.06TLNTLKK16 pKa = 10.72EE17 pKa = 3.99KK18 pKa = 10.09TFNLTNFSNLNAWQLMVDD36 pKa = 4.69GARR39 pKa = 11.84KK40 pKa = 8.64RR41 pKa = 11.84PKK43 pKa = 8.2YY44 pKa = 7.97FHH46 pKa = 7.01RR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84EE50 pKa = 4.2TVLSNVAGSLTEE62 pKa = 3.87HH63 pKa = 7.14KK64 pKa = 10.61LGIFTIEE71 pKa = 4.08DD72 pKa = 3.23VRR74 pKa = 11.84NVKK77 pKa = 9.94SYY79 pKa = 10.92KK80 pKa = 9.3FLRR83 pKa = 11.84IVGIQIKK90 pKa = 8.11VTSHH94 pKa = 6.88LPRR97 pKa = 11.84DD98 pKa = 3.62TSGFLQIDD106 pKa = 4.91LIDD109 pKa = 3.75SRR111 pKa = 11.84LTDD114 pKa = 3.12GRR116 pKa = 11.84KK117 pKa = 9.21RR118 pKa = 11.84SKK120 pKa = 10.05VLQRR124 pKa = 11.84FVAKK128 pKa = 10.55ACDD131 pKa = 3.57NTSVVQYY138 pKa = 10.88KK139 pKa = 10.14FDD141 pKa = 3.83YY142 pKa = 10.67CVSTRR147 pKa = 11.84EE148 pKa = 4.25NIADD152 pKa = 3.49LWKK155 pKa = 10.48IGTVAVNVPVVDD167 pKa = 3.49DD168 pKa = 4.73CYY170 pKa = 10.31PFSVEE175 pKa = 5.04VSMIWVATDD184 pKa = 2.87STTRR188 pKa = 11.84LNVEE192 pKa = 4.46EE193 pKa = 5.17LNQTDD198 pKa = 3.93YY199 pKa = 11.97LEE201 pKa = 5.77GEE203 pKa = 4.45FSDD206 pKa = 5.19QEE208 pKa = 4.17MFDD211 pKa = 3.17TFMDD215 pKa = 4.35LKK217 pKa = 9.79HH218 pKa = 7.99AEE220 pKa = 4.19MKK222 pKa = 10.83SVDD225 pKa = 3.47VKK227 pKa = 11.05FKK229 pKa = 11.39GNYY232 pKa = 8.49LPKK235 pKa = 10.63ASVDD239 pKa = 3.67RR240 pKa = 11.84NISNMEE246 pKa = 4.87RR247 pKa = 11.84IPDD250 pKa = 4.09SIKK253 pKa = 10.61KK254 pKa = 10.15SGSIKK259 pKa = 10.38KK260 pKa = 10.06KK261 pKa = 9.93RR262 pKa = 3.4

Molecular weight: 30.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

4056

107

1766

579.4

65.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.46 ± 0.445

2.145 ± 0.389

7.569 ± 0.432

6.016 ± 0.308

5.153 ± 0.295

5.227 ± 0.468

1.627 ± 0.139

4.734 ± 0.196

8.037 ± 0.391

9.048 ± 0.475

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.687 ± 0.213

4.783 ± 0.317

3.008 ± 0.121

3.6 ± 0.389

5.769 ± 0.173

7.027 ± 0.403

5.104 ± 0.32

7.717 ± 0.293

1.282 ± 0.088

2.983 ± 0.281
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski