Microviridae sp. ctKAt32

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2WAI9|A0A5Q2WAI9_9VIRU Putative minor capsid protein OS=Microviridae sp. ctKAt32 OX=2656697 PE=4 SV=1
MM1 pKa = 7.26RR2 pKa = 11.84TNIFSVYY9 pKa = 10.04DD10 pKa = 3.92SKK12 pKa = 11.64ALCYY16 pKa = 9.8GVPFFMATVGAAVRR30 pKa = 11.84AFQDD34 pKa = 2.97VANDD38 pKa = 3.32VNSVINRR45 pKa = 11.84HH46 pKa = 5.67PMDD49 pKa = 3.84FVLYY53 pKa = 10.44KK54 pKa = 10.28IGEE57 pKa = 4.04FDD59 pKa = 3.69DD60 pKa = 3.82EE61 pKa = 4.55KK62 pKa = 11.52GIVEE66 pKa = 4.21SLQPFISLGHH76 pKa = 6.4GGDD79 pKa = 3.54FVKK82 pKa = 10.51PVSGVLKK89 pKa = 8.48MTEE92 pKa = 4.07GVKK95 pKa = 10.49

Molecular weight: 10.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2WAF2|A0A5Q2WAF2_9VIRU DNA binding protein OS=Microviridae sp. ctKAt32 OX=2656697 PE=4 SV=1
MM1 pKa = 7.38GCSSPQLVFFPEE13 pKa = 3.9KK14 pKa = 10.62GKK16 pKa = 10.31MSVACGTCEE25 pKa = 3.91GCIIKK30 pKa = 10.39HH31 pKa = 5.73KK32 pKa = 10.51EE33 pKa = 3.49YY34 pKa = 10.11LAQMRR39 pKa = 11.84LKK41 pKa = 10.5FFQLMGGNYY50 pKa = 9.16VKK52 pKa = 9.94KK53 pKa = 9.14TRR55 pKa = 11.84NRR57 pKa = 11.84SS58 pKa = 3.13

Molecular weight: 6.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1423

58

536

237.2

26.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.784 ± 1.957

1.476 ± 0.671

5.411 ± 0.483

5.341 ± 0.714

5.341 ± 0.779

7.941 ± 0.599

1.546 ± 0.414

4.216 ± 0.394

5.833 ± 1.508

6.535 ± 0.36

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.865 ± 1.199

4.779 ± 0.236

5.341 ± 0.742

3.865 ± 0.851

6.114 ± 0.748

6.887 ± 0.584

4.779 ± 1.296

6.887 ± 0.972

0.914 ± 0.31

4.146 ± 0.559
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski