Burkholderia virus phiE202

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Tigrvirus

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4JWT8|A4JWT8_9CAUD Gp41 LysC OS=Burkholderia virus phiE202 OX=431893 GN=BTHphiE202_0041 PE=4 SV=1
MM1 pKa = 7.55NKK3 pKa = 9.72PSSLRR8 pKa = 11.84AALVAALPQLNASPDD23 pKa = 3.54QLLVFVNEE31 pKa = 4.08GRR33 pKa = 11.84IEE35 pKa = 4.06ATGTRR40 pKa = 11.84TASFDD45 pKa = 3.57YY46 pKa = 10.55EE47 pKa = 4.45YY48 pKa = 10.09EE49 pKa = 4.1CEE51 pKa = 3.94IIIRR55 pKa = 11.84DD56 pKa = 4.52FIGNPDD62 pKa = 3.7DD63 pKa = 4.59VMIAVVEE70 pKa = 4.21WARR73 pKa = 11.84ANQPDD78 pKa = 4.44LVTNRR83 pKa = 11.84DD84 pKa = 3.08EE85 pKa = 4.6RR86 pKa = 11.84RR87 pKa = 11.84NGMTFVADD95 pKa = 3.33ILSNNAVDD103 pKa = 5.23LGLKK107 pKa = 9.89VKK109 pKa = 10.66LSEE112 pKa = 4.35SVVVGTDD119 pKa = 2.99EE120 pKa = 4.33AGNRR124 pKa = 11.84TVEE127 pKa = 4.78HH128 pKa = 6.93IDD130 pKa = 3.61DD131 pKa = 5.43AADD134 pKa = 3.14EE135 pKa = 4.32WLSS138 pKa = 3.44

Molecular weight: 15.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4JWQ8|A4JWQ8_9CAUD Toprim domain-containing protein OS=Burkholderia virus phiE202 OX=431893 GN=BTHphiE202_0011 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84FYY4 pKa = 11.02KK5 pKa = 10.16RR6 pKa = 11.84QKK8 pKa = 9.85NGPWWFDD15 pKa = 3.05LSIDD19 pKa = 3.7GSRR22 pKa = 11.84IRR24 pKa = 11.84QSSGTSDD31 pKa = 2.75RR32 pKa = 11.84RR33 pKa = 11.84AAEE36 pKa = 3.71EE37 pKa = 3.76LAAKK41 pKa = 9.58VASDD45 pKa = 2.99YY46 pKa = 10.33WRR48 pKa = 11.84QKK50 pKa = 11.12KK51 pKa = 10.21LGEE54 pKa = 4.39RR55 pKa = 11.84PTVTWDD61 pKa = 3.01AAVVHH66 pKa = 6.13WLKK69 pKa = 10.89QNQHH73 pKa = 4.62QRR75 pKa = 11.84SLEE78 pKa = 4.1TTKK81 pKa = 10.4QRR83 pKa = 11.84LRR85 pKa = 11.84WLTDD89 pKa = 3.02QLKK92 pKa = 10.66GEE94 pKa = 4.22NVRR97 pKa = 11.84NIDD100 pKa = 3.52RR101 pKa = 11.84EE102 pKa = 4.15RR103 pKa = 11.84IQALIEE109 pKa = 3.81TKK111 pKa = 10.45AAEE114 pKa = 4.5KK115 pKa = 10.88YY116 pKa = 10.03RR117 pKa = 11.84GSPVAGATVNRR128 pKa = 11.84HH129 pKa = 4.03MAALSVILHH138 pKa = 6.28HH139 pKa = 6.37CHH141 pKa = 6.46AEE143 pKa = 3.93GWIDD147 pKa = 3.67AVPPIRR153 pKa = 11.84KK154 pKa = 8.83LRR156 pKa = 11.84EE157 pKa = 3.33NSARR161 pKa = 11.84LTWLTRR167 pKa = 11.84AQAQRR172 pKa = 11.84LLEE175 pKa = 4.15EE176 pKa = 4.95LPTHH180 pKa = 6.55LRR182 pKa = 11.84QMARR186 pKa = 11.84FALATGLRR194 pKa = 11.84EE195 pKa = 4.27SNVRR199 pKa = 11.84LLEE202 pKa = 3.9WAQVDD207 pKa = 4.41RR208 pKa = 11.84EE209 pKa = 4.03RR210 pKa = 11.84ALAWIHH216 pKa = 6.96ADD218 pKa = 3.13QAKK221 pKa = 9.28AGKK224 pKa = 9.85VISVPLNEE232 pKa = 4.97DD233 pKa = 2.97ALGVLRR239 pKa = 11.84EE240 pKa = 3.99QQGQHH245 pKa = 4.65KK246 pKa = 10.04RR247 pKa = 11.84YY248 pKa = 8.69VFVYY252 pKa = 9.12KK253 pKa = 10.36GAPIGRR259 pKa = 11.84IYY261 pKa = 10.26NHH263 pKa = 6.83AWQKK267 pKa = 9.5ACVRR271 pKa = 11.84AGLSGLRR278 pKa = 11.84FHH280 pKa = 7.58DD281 pKa = 5.35LRR283 pKa = 11.84HH284 pKa = 5.71TWASWHH290 pKa = 5.46VQAGTPLPILQQLGGWASYY309 pKa = 10.9QMVLRR314 pKa = 11.84YY315 pKa = 9.69AHH317 pKa = 7.51LGRR320 pKa = 11.84DD321 pKa = 3.49HH322 pKa = 6.31VAAYY326 pKa = 10.25ADD328 pKa = 3.74NIGTLRR334 pKa = 11.84HH335 pKa = 6.08KK336 pKa = 10.73SGTPPEE342 pKa = 4.1IEE344 pKa = 4.42KK345 pKa = 11.01GSDD348 pKa = 3.24CSEE351 pKa = 4.09PLSNLVAWGGIEE363 pKa = 3.84PPTRR367 pKa = 11.84GFSKK371 pKa = 9.85RR372 pKa = 11.84TADD375 pKa = 3.31TSAPSGKK382 pKa = 10.14ASRR385 pKa = 11.84PPKK388 pKa = 9.56KK389 pKa = 10.13RR390 pKa = 11.84KK391 pKa = 9.06AAA393 pKa = 3.76

Molecular weight: 44.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

10750

37

988

224.0

24.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.712 ± 0.565

0.828 ± 0.138

6.344 ± 0.336

5.321 ± 0.301

3.386 ± 0.242

7.656 ± 0.52

2.316 ± 0.214

4.019 ± 0.213

3.935 ± 0.264

8.837 ± 0.426

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.353 ± 0.249

2.986 ± 0.219

4.735 ± 0.293

3.414 ± 0.261

8.223 ± 0.363

5.051 ± 0.264

5.935 ± 0.269

6.893 ± 0.32

1.581 ± 0.168

2.474 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski