Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus; Alkalihalobacillus halodurans

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4006 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9K8R8|Q9K8R8_BACHD Aryldialkylphosphatase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=BH2935 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 10.26KK3 pKa = 10.37KK4 pKa = 10.74LGFLSAGFLSLGLILGACGGNADD27 pKa = 4.17TDD29 pKa = 3.91AEE31 pKa = 4.54EE32 pKa = 4.42PAGDD36 pKa = 4.19DD37 pKa = 3.5PADD40 pKa = 3.46TGEE43 pKa = 4.26EE44 pKa = 4.01QEE46 pKa = 4.54EE47 pKa = 4.17AAGDD51 pKa = 4.14HH52 pKa = 6.24LTDD55 pKa = 3.98LQLGTGSTGGTYY67 pKa = 10.26YY68 pKa = 10.54PLGQEE73 pKa = 3.87IANVLNANVDD83 pKa = 3.45YY84 pKa = 11.52DD85 pKa = 4.07GFNVSAVASGASVDD99 pKa = 3.56NLGQIFRR106 pKa = 11.84GEE108 pKa = 4.18MQLGMTVHH116 pKa = 6.8IPALQAIAGEE126 pKa = 4.48GDD128 pKa = 3.4FEE130 pKa = 4.77GAVVDD135 pKa = 3.75NFGFLGYY142 pKa = 9.92IYY144 pKa = 9.67PEE146 pKa = 4.03VMQVVTTEE154 pKa = 4.06DD155 pKa = 2.87TGITSIEE162 pKa = 4.03DD163 pKa = 3.4LKK165 pKa = 11.14GKK167 pKa = 10.09RR168 pKa = 11.84VAIGPAGSATQSAARR183 pKa = 11.84LILEE187 pKa = 3.88AHH189 pKa = 6.98GIEE192 pKa = 4.75DD193 pKa = 3.34GDD195 pKa = 3.88YY196 pKa = 10.22EE197 pKa = 4.3AFEE200 pKa = 4.59EE201 pKa = 5.1GFGDD205 pKa = 3.64AAARR209 pKa = 11.84IQDD212 pKa = 3.77GQLDD216 pKa = 3.81ASFGLLGLPDD226 pKa = 3.77STTSEE231 pKa = 4.12LSLQRR236 pKa = 11.84EE237 pKa = 4.51VVILPIEE244 pKa = 4.54GDD246 pKa = 2.97ALAYY250 pKa = 10.58VEE252 pKa = 4.83EE253 pKa = 4.38NSDD256 pKa = 3.48YY257 pKa = 11.21GAYY260 pKa = 10.1EE261 pKa = 4.04IDD263 pKa = 3.07AGAYY267 pKa = 9.82DD268 pKa = 4.26FLDD271 pKa = 4.34DD272 pKa = 4.49SVSTVTAYY280 pKa = 10.55AILVASLDD288 pKa = 3.77QVSEE292 pKa = 4.08DD293 pKa = 3.31LGYY296 pKa = 10.89EE297 pKa = 3.95IVKK300 pKa = 10.45ALYY303 pKa = 9.72EE304 pKa = 4.03NAGGITHH311 pKa = 6.85PQGEE315 pKa = 4.52HH316 pKa = 4.66MKK318 pKa = 9.91MEE320 pKa = 5.03NILNGSEE327 pKa = 4.25GLPIHH332 pKa = 6.98PGAQKK337 pKa = 10.39YY338 pKa = 7.52FQEE341 pKa = 4.21QGISEE346 pKa = 4.22

Molecular weight: 36.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9Z9P6|REX_BACHD Redox-sensing transcriptional repressor Rex OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=rex PE=3 SV=1
MM1 pKa = 7.71GKK3 pKa = 8.0PTFQPNNRR11 pKa = 11.84KK12 pKa = 9.23RR13 pKa = 11.84KK14 pKa = 8.22KK15 pKa = 8.69VHH17 pKa = 5.46GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTKK26 pKa = 10.19NGRR29 pKa = 11.84KK30 pKa = 8.75VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.2KK38 pKa = 10.05GRR40 pKa = 11.84KK41 pKa = 8.66VLSAA45 pKa = 4.05

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4006

0

4006

1169503

11

1816

291.9

32.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.416 ± 0.04

0.726 ± 0.011

5.089 ± 0.031

7.844 ± 0.05

4.451 ± 0.032

7.043 ± 0.042

2.413 ± 0.021

6.942 ± 0.034

5.805 ± 0.034

10.017 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.776 ± 0.017

3.62 ± 0.022

3.833 ± 0.023

4.102 ± 0.029

4.772 ± 0.033

5.629 ± 0.025

5.558 ± 0.026

7.474 ± 0.036

1.132 ± 0.018

3.361 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski