Rheinheimera sp. SA_1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiaceae; Rheinheimera; unclassified Rheinheimera

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4186 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B7UHQ5|A0A1B7UHQ5_9GAMM Riboflavin biosynthesis protein OS=Rheinheimera sp. SA_1 OX=1827365 GN=A5320_15215 PE=3 SV=1
MM1 pKa = 7.25SLKK4 pKa = 10.28IAVFYY9 pKa = 10.99GSTTCYY15 pKa = 10.73TEE17 pKa = 3.71MTAEE21 pKa = 4.98KK22 pKa = 9.74IQALLAVDD30 pKa = 3.98AALPTGSSVTLHH42 pKa = 6.26NLKK45 pKa = 9.42QQPLATMANYY55 pKa = 10.13DD56 pKa = 3.79VLILGISTWDD66 pKa = 3.44FGEE69 pKa = 4.43LQEE72 pKa = 5.27DD73 pKa = 4.61WEE75 pKa = 4.39AHH77 pKa = 5.72WDD79 pKa = 4.03DD80 pKa = 3.82IAAVDD85 pKa = 4.03LSGKK89 pKa = 9.58IVAIYY94 pKa = 10.73GMGDD98 pKa = 3.17QLGYY102 pKa = 11.14AEE104 pKa = 4.8WFVDD108 pKa = 4.91AIGMLHH114 pKa = 5.81QAISDD119 pKa = 3.89QPCQRR124 pKa = 11.84IGFWSTQGYY133 pKa = 10.22DD134 pKa = 3.76FIKK137 pKa = 10.74SKK139 pKa = 11.09AVTDD143 pKa = 4.04DD144 pKa = 4.22GEE146 pKa = 4.34WFYY149 pKa = 11.91GLALDD154 pKa = 5.65EE155 pKa = 4.68EE156 pKa = 4.49NQYY159 pKa = 11.51DD160 pKa = 3.71QTDD163 pKa = 3.43EE164 pKa = 5.19RR165 pKa = 11.84LSLWLNQIVEE175 pKa = 4.24EE176 pKa = 4.25MIEE179 pKa = 4.11LVV181 pKa = 3.4

Molecular weight: 20.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B7UPC2|A0A1B7UPC2_9GAMM HlyD_D23 domain-containing protein OS=Rheinheimera sp. SA_1 OX=1827365 GN=A5320_02635 PE=4 SV=1
MM1 pKa = 7.7GSPFFCWRR9 pKa = 11.84LLALTLAPGTKK20 pKa = 9.66ARR22 pKa = 11.84QRR24 pKa = 11.84QIRR27 pKa = 11.84QRR29 pKa = 11.84LQSGNILLVKK39 pKa = 10.45RR40 pKa = 11.84SGPGKK45 pKa = 8.35TLLLPALVPARR56 pKa = 11.84WRR58 pKa = 11.84FSPVLFLMRR67 pKa = 11.84LQFF70 pKa = 4.12

Molecular weight: 8.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4186

0

4186

1508662

37

7335

360.4

39.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.244 ± 0.042

0.994 ± 0.012

5.117 ± 0.031

5.029 ± 0.033

4.039 ± 0.026

6.653 ± 0.034

2.214 ± 0.019

5.175 ± 0.032

4.243 ± 0.041

11.996 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.242 ± 0.019

3.602 ± 0.028

4.432 ± 0.027

6.538 ± 0.06

4.719 ± 0.028

6.313 ± 0.03

5.257 ± 0.031

6.824 ± 0.03

1.435 ± 0.019

2.934 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski