Arthrobacter subterraneus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Arthrobacter

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3624 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G8KK19|A0A1G8KK19_9MICC Succinate semialdehyde dehydrogenase OS=Arthrobacter subterraneus OX=335973 GN=SAMN04488693_11168 PE=3 SV=1
MM1 pKa = 6.79THH3 pKa = 5.84SHH5 pKa = 6.74PARR8 pKa = 11.84RR9 pKa = 11.84SPLRR13 pKa = 11.84ALAGAAAVVVGLVLSGCTAGPDD35 pKa = 3.54TGAVTAPPEE44 pKa = 4.35VIGEE48 pKa = 4.16VPEE51 pKa = 4.29DD52 pKa = 3.42LRR54 pKa = 11.84EE55 pKa = 4.19FYY57 pKa = 10.75AQEE60 pKa = 4.27VTWEE64 pKa = 4.12EE65 pKa = 4.54CEE67 pKa = 4.47GDD69 pKa = 4.32DD70 pKa = 5.19FACATVKK77 pKa = 10.57VPLDD81 pKa = 3.45YY82 pKa = 11.03SDD84 pKa = 4.6PGRR87 pKa = 11.84ASIDD91 pKa = 2.9IAMIRR96 pKa = 11.84LDD98 pKa = 4.4APGDD102 pKa = 3.81AQGSLLVNPGGPGVSGYY119 pKa = 11.17NLVRR123 pKa = 11.84DD124 pKa = 3.72SAAFILSDD132 pKa = 4.09RR133 pKa = 11.84LRR135 pKa = 11.84QSFDD139 pKa = 3.18VVGFDD144 pKa = 3.37PRR146 pKa = 11.84GVNEE150 pKa = 4.16SSAVEE155 pKa = 4.1CLTDD159 pKa = 3.48EE160 pKa = 4.52EE161 pKa = 4.67RR162 pKa = 11.84DD163 pKa = 3.4EE164 pKa = 5.03ARR166 pKa = 11.84AEE168 pKa = 4.11TLPPDD173 pKa = 3.87ATDD176 pKa = 4.06AEE178 pKa = 4.21ALEE181 pKa = 4.19ILEE184 pKa = 4.79ADD186 pKa = 3.53SAEE189 pKa = 4.14YY190 pKa = 10.51AQLCAEE196 pKa = 4.32RR197 pKa = 11.84TGEE200 pKa = 4.09LLGHH204 pKa = 7.11IDD206 pKa = 3.58TVSAAKK212 pKa = 10.9DD213 pKa = 3.4MDD215 pKa = 3.52ILRR218 pKa = 11.84AVLGDD223 pKa = 3.5EE224 pKa = 3.91QLNYY228 pKa = 10.92LGFSYY233 pKa = 8.74GTQLGAAYY241 pKa = 10.26AGLFPGNVGRR251 pKa = 11.84FVLDD255 pKa = 3.69GAVDD259 pKa = 4.12PSLSSAEE266 pKa = 3.8ITAGQVAAFDD276 pKa = 3.67EE277 pKa = 4.39ALRR280 pKa = 11.84NYY282 pKa = 9.87VADD285 pKa = 3.99CQSQSEE291 pKa = 4.67CPLPGNVDD299 pKa = 3.18EE300 pKa = 5.38GVAVVDD306 pKa = 3.87EE307 pKa = 4.57LFEE310 pKa = 4.39SVEE313 pKa = 4.14EE314 pKa = 4.43SPMTAADD321 pKa = 3.92GRR323 pKa = 11.84PVTIGTFFQGFVLPLYY339 pKa = 10.75DD340 pKa = 3.69SASWPALTAAIDD352 pKa = 3.45QALQGDD358 pKa = 4.0PSEE361 pKa = 4.52FLRR364 pKa = 11.84FADD367 pKa = 4.44LGADD371 pKa = 3.85RR372 pKa = 11.84SPTGDD377 pKa = 3.56YY378 pKa = 8.55LTNSAAAFTAVTCLDD393 pKa = 3.85YY394 pKa = 11.23PMPADD399 pKa = 3.98PGVMAEE405 pKa = 3.97EE406 pKa = 4.59AEE408 pKa = 4.09ALEE411 pKa = 4.44ASSAVFGEE419 pKa = 4.12YY420 pKa = 10.4LSYY423 pKa = 11.15GALACKK429 pKa = 9.69NWAYY433 pKa = 10.52EE434 pKa = 4.13PVNEE438 pKa = 4.7PSPASAPGAPPMLVIGTTGDD458 pKa = 3.36PATPYY463 pKa = 9.88EE464 pKa = 4.19WSLALAEE471 pKa = 4.11QLEE474 pKa = 4.9SAVHH478 pKa = 4.68VTWEE482 pKa = 4.56GQGHH486 pKa = 5.01TAYY489 pKa = 10.14GRR491 pKa = 11.84SGEE494 pKa = 4.41CIEE497 pKa = 4.92SLVDD501 pKa = 4.77DD502 pKa = 4.49FLIDD506 pKa = 3.45GTVPEE511 pKa = 4.47MSARR515 pKa = 11.84CC516 pKa = 3.57

Molecular weight: 54.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G8GL13|A0A1G8GL13_9MICC Myo-inositol-1(Or 4)-monophosphatase OS=Arthrobacter subterraneus OX=335973 GN=SAMN04488693_104171 PE=4 SV=1
MM1 pKa = 7.4GLSNAIRR8 pKa = 11.84GAAGRR13 pKa = 11.84FTGRR17 pKa = 11.84SGGTTGRR24 pKa = 11.84TPRR27 pKa = 11.84TGGMGTGRR35 pKa = 11.84GGVSRR40 pKa = 11.84GGSGGVGGILRR51 pKa = 11.84GILRR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 3.46

Molecular weight: 5.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3624

0

3624

1160108

19

2041

320.1

34.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.554 ± 0.056

0.616 ± 0.009

5.738 ± 0.035

6.044 ± 0.038

3.169 ± 0.025

8.972 ± 0.035

2.113 ± 0.018

4.39 ± 0.032

2.318 ± 0.028

10.311 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.951 ± 0.018

2.461 ± 0.021

5.444 ± 0.033

3.11 ± 0.02

6.939 ± 0.043

5.992 ± 0.027

5.946 ± 0.03

8.429 ± 0.038

1.42 ± 0.019

2.083 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski