Rheinheimera salexigens

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiaceae; Rheinheimera

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3027 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E7Q8K2|A0A1E7Q8K2_9GAMM Metalloendopeptidase OS=Rheinheimera salexigens OX=1628148 GN=BI198_13755 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.53LNTVHH7 pKa = 6.88HH8 pKa = 6.86AGIAMALLLGVSGTATASLISGFEE32 pKa = 4.12TQFDD36 pKa = 3.66TDD38 pKa = 3.76FAVAGVGGLRR48 pKa = 11.84GNGFGDD54 pKa = 3.34INLTGVVGTVTKK66 pKa = 10.52AYY68 pKa = 10.03LYY70 pKa = 9.42WHH72 pKa = 7.24GPTDD76 pKa = 3.65STDD79 pKa = 3.46PNFNANLTMNGNAVSGTNIGFSDD102 pKa = 3.95DD103 pKa = 4.12NFWGQEE109 pKa = 3.38NSQAYY114 pKa = 9.17RR115 pKa = 11.84ADD117 pKa = 3.38VTSLVNADD125 pKa = 3.42GTYY128 pKa = 11.05SLAGLSAANSNGASLVVYY146 pKa = 10.52YY147 pKa = 10.76DD148 pKa = 5.45DD149 pKa = 5.93GDD151 pKa = 3.93STNNRR156 pKa = 11.84DD157 pKa = 3.1IVSFDD162 pKa = 3.5GNDD165 pKa = 3.12ANFANTYY172 pKa = 10.79DD173 pKa = 3.77PLGWLVEE180 pKa = 4.01LSGINYY186 pKa = 10.19SGGDD190 pKa = 3.38AFLTMSVSDD199 pKa = 4.04GQDD202 pKa = 2.77FGLGDD207 pKa = 4.08DD208 pKa = 5.87GIFFLNGIALNTADD222 pKa = 5.05LFDD225 pKa = 4.6GATVPQTPGSSVTNGGLWDD244 pKa = 4.02IVTIDD249 pKa = 3.14ITSFLNIGLNSLSLTHH265 pKa = 7.24SSEE268 pKa = 3.88TDD270 pKa = 3.04ALSAIHH276 pKa = 6.34FAINLPEE283 pKa = 4.44GAAPDD288 pKa = 3.98PTDD291 pKa = 3.67PTDD294 pKa = 3.53IPEE297 pKa = 4.22PAVWSLFLLALYY309 pKa = 9.77FGRR312 pKa = 11.84KK313 pKa = 6.67TLRR316 pKa = 11.84LSS318 pKa = 3.29

Molecular weight: 33.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E7Q2B1|A0A1E7Q2B1_9GAMM Ubiquinone biosynthesis accessory factor UbiJ OS=Rheinheimera salexigens OX=1628148 GN=ubiJ PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.98RR4 pKa = 11.84TFQPSVLKK12 pKa = 10.46RR13 pKa = 11.84KK14 pKa = 9.37RR15 pKa = 11.84NHH17 pKa = 5.37GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.3NGRR29 pKa = 11.84AVLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.25GRR40 pKa = 11.84QRR42 pKa = 11.84LSAA45 pKa = 4.04

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3027

0

3027

992880

37

2539

328.0

36.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.826 ± 0.047

0.943 ± 0.015

5.257 ± 0.035

5.2 ± 0.041

4.022 ± 0.031

6.365 ± 0.047

2.185 ± 0.022

6.366 ± 0.031

4.85 ± 0.04

11.189 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.38 ± 0.023

4.236 ± 0.03

3.913 ± 0.023

6.008 ± 0.063

4.456 ± 0.029

6.462 ± 0.038

5.356 ± 0.035

6.662 ± 0.036

1.258 ± 0.018

3.066 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski