Gordonibacter urolithinfaciens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group;

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2703 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A423UN21|A0A423UN21_9ACTN LysR family transcriptional regulator OS=Gordonibacter urolithinfaciens OX=1335613 GN=DMP12_02610 PE=3 SV=1
MM1 pKa = 7.56LFFGISNLFSTCSATPTEE19 pKa = 4.41EE20 pKa = 3.98EE21 pKa = 3.9PAAEE25 pKa = 4.94AEE27 pKa = 4.5AEE29 pKa = 4.31SPPTPEE35 pKa = 3.97EE36 pKa = 3.73RR37 pKa = 11.84LAALPAVEE45 pKa = 4.53TGLAEE50 pKa = 4.8LDD52 pKa = 4.15LPTGTEE58 pKa = 3.93PQAFAPAPAEE68 pKa = 4.14GSAVAVQPVLSDD80 pKa = 3.38ASKK83 pKa = 10.93AAIDD87 pKa = 3.44QAFAPFAEE95 pKa = 4.4NDD97 pKa = 3.37RR98 pKa = 11.84SFGFALVDD106 pKa = 4.12LGTGRR111 pKa = 11.84GIASNVDD118 pKa = 2.95AVFYY122 pKa = 10.33GASSFKK128 pKa = 10.85GPYY131 pKa = 9.04CVYY134 pKa = 10.06FCQSQLEE141 pKa = 4.51TGLATRR147 pKa = 11.84DD148 pKa = 3.68DD149 pKa = 4.29FVTSGFMSEE158 pKa = 4.21SGVFSSQGSEE168 pKa = 4.65LIGSMIEE175 pKa = 4.03SAILFSSNDD184 pKa = 3.12TYY186 pKa = 11.66GALRR190 pKa = 11.84YY191 pKa = 9.96DD192 pKa = 3.96YY193 pKa = 11.42SDD195 pKa = 4.33DD196 pKa = 3.78ALAAYY201 pKa = 10.51LEE203 pKa = 4.4GLGVDD208 pKa = 3.61PALAYY213 pKa = 7.76DD214 pKa = 3.57TWFPHH219 pKa = 4.22YY220 pKa = 8.51TVRR223 pKa = 11.84DD224 pKa = 4.09GVRR227 pKa = 11.84LWPNASEE234 pKa = 4.15YY235 pKa = 10.48LASGTEE241 pKa = 4.04TAQWLAGLLEE251 pKa = 4.46RR252 pKa = 11.84DD253 pKa = 3.19EE254 pKa = 4.35TSYY257 pKa = 11.25LRR259 pKa = 11.84EE260 pKa = 3.92ALKK263 pKa = 11.02DD264 pKa = 3.26EE265 pKa = 4.62GVQVQSKK272 pKa = 9.09PGWSMEE278 pKa = 3.94SFEE281 pKa = 4.24NGEE284 pKa = 4.08YY285 pKa = 10.54NALCDD290 pKa = 3.95AGIVAKK296 pKa = 10.46DD297 pKa = 3.2GRR299 pKa = 11.84RR300 pKa = 11.84YY301 pKa = 9.82LVSVMSNIPYY311 pKa = 6.99TTSNVDD317 pKa = 3.35LFEE320 pKa = 4.92DD321 pKa = 4.05VIAAVFAARR330 pKa = 11.84GDD332 pKa = 3.87LAA334 pKa = 5.89

Molecular weight: 35.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A423UM63|A0A423UM63_9ACTN Na(+)/H(+) antiporter NhaA OS=Gordonibacter urolithinfaciens OX=1335613 GN=nhaA PE=3 SV=1
MM1 pKa = 7.07VRR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84PPLSRR10 pKa = 11.84NRR12 pKa = 11.84SGPPSKK18 pKa = 10.22RR19 pKa = 11.84ARR21 pKa = 11.84SSRR24 pKa = 11.84KK25 pKa = 8.93RR26 pKa = 11.84VPSRR30 pKa = 11.84LRR32 pKa = 11.84RR33 pKa = 11.84PKK35 pKa = 10.69APSPLPARR43 pKa = 11.84QPRR46 pKa = 11.84PSRR49 pKa = 11.84PSRR52 pKa = 11.84PIWRR56 pKa = 11.84RR57 pKa = 11.84SMEE60 pKa = 4.18LPRR63 pKa = 11.84GASTRR68 pKa = 11.84RR69 pKa = 11.84SPRR72 pKa = 3.06

Molecular weight: 8.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2703

0

2703

921810

37

3347

341.0

36.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.755 ± 0.074

1.615 ± 0.027

6.005 ± 0.041

6.539 ± 0.043

3.89 ± 0.036

8.67 ± 0.043

1.751 ± 0.018

4.39 ± 0.038

3.543 ± 0.04

9.507 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.531 ± 0.026

2.609 ± 0.028

4.702 ± 0.033

2.718 ± 0.027

6.236 ± 0.048

5.333 ± 0.04

4.952 ± 0.043

8.255 ± 0.048

1.16 ± 0.022

2.838 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski