Apis mellifera associated microvirus 24

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8U5Q7|A0A3Q8U5Q7_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 24 OX=2494752 PE=3 SV=1
MM1 pKa = 7.37NLGSRR6 pKa = 11.84YY7 pKa = 8.46SQHH10 pKa = 6.61SFAQIPDD17 pKa = 3.26VNMARR22 pKa = 11.84SKK24 pKa = 10.55FNRR27 pKa = 11.84SFAVKK32 pKa = 9.34DD33 pKa = 3.39TFNFDD38 pKa = 3.49DD39 pKa = 4.76LVPFFIDD46 pKa = 4.33EE47 pKa = 5.08IIPGDD52 pKa = 4.04TINLNVKK59 pKa = 9.77SFSRR63 pKa = 11.84LAPQVVPLMDD73 pKa = 4.32NMYY76 pKa = 9.42MDD78 pKa = 3.79YY79 pKa = 11.13FFFFVPNRR87 pKa = 11.84LVWNNWEE94 pKa = 4.4KK95 pKa = 11.01FCGAQDD101 pKa = 4.2DD102 pKa = 4.97PGDD105 pKa = 3.82STDD108 pKa = 3.91YY109 pKa = 10.3IVPRR113 pKa = 11.84IALAEE118 pKa = 4.03TGANVVGSIYY128 pKa = 10.71DD129 pKa = 3.37KK130 pKa = 11.16FGIPTDD136 pKa = 3.87VPDD139 pKa = 3.6VTINALPFRR148 pKa = 11.84AYY150 pKa = 11.2NLIFNSWFRR159 pKa = 11.84DD160 pKa = 3.4QNLQDD165 pKa = 4.02SLPVPKK171 pKa = 9.86TDD173 pKa = 5.11GPDD176 pKa = 3.14NPADD180 pKa = 3.78YY181 pKa = 10.63IIVQRR186 pKa = 11.84GKK188 pKa = 9.15RR189 pKa = 11.84HH190 pKa = 7.0DD191 pKa = 4.22YY192 pKa = 8.15FTSALPWPQKK202 pKa = 10.85GEE204 pKa = 3.86AVTIPTGGLAPVVGTITGPSSLAVLPYY231 pKa = 10.02RR232 pKa = 11.84ANTGAFPDD240 pKa = 3.55ANIRR244 pKa = 11.84SGVASTTGTAIFASGTSLPNNTDD267 pKa = 2.81INPYY271 pKa = 9.75ISAGGLTMSHH281 pKa = 5.91TLEE284 pKa = 4.92ADD286 pKa = 3.43LTEE289 pKa = 4.42STAITINQFRR299 pKa = 11.84LAMMTQSFLEE309 pKa = 3.97LDD311 pKa = 3.34ARR313 pKa = 11.84GGTRR317 pKa = 11.84YY318 pKa = 10.04VEE320 pKa = 4.23ILRR323 pKa = 11.84AHH325 pKa = 6.15YY326 pKa = 10.55NVVSPDD332 pKa = 3.2FRR334 pKa = 11.84LQRR337 pKa = 11.84PEE339 pKa = 4.0LLSVGSSTISQHH351 pKa = 6.31VVAQNSATTEE361 pKa = 3.88TSPQGNLAAFSTAYY375 pKa = 9.37TGSSRR380 pKa = 11.84IGFSKK385 pKa = 10.71SFVEE389 pKa = 3.84HH390 pKa = 6.77GYY392 pKa = 11.01VIGLVAARR400 pKa = 11.84ADD402 pKa = 3.48LTYY405 pKa = 10.67QQGLNRR411 pKa = 11.84MWSRR415 pKa = 11.84TTRR418 pKa = 11.84QDD420 pKa = 3.34FFWPKK425 pKa = 9.43FQEE428 pKa = 4.26LGEE431 pKa = 3.86QAIFNRR437 pKa = 11.84EE438 pKa = 3.33IFYY441 pKa = 11.13SGVAEE446 pKa = 5.28DD447 pKa = 4.51DD448 pKa = 4.02DD449 pKa = 5.47AVFGYY454 pKa = 7.42QEE456 pKa = 4.17RR457 pKa = 11.84YY458 pKa = 9.58AEE460 pKa = 4.06YY461 pKa = 9.72RR462 pKa = 11.84YY463 pKa = 10.26KK464 pKa = 10.54PSEE467 pKa = 3.37IRR469 pKa = 11.84GQFRR473 pKa = 11.84STYY476 pKa = 11.11AEE478 pKa = 4.14TLDD481 pKa = 3.5VWHH484 pKa = 6.78LAEE487 pKa = 5.01EE488 pKa = 5.01FSSTPALNGEE498 pKa = 4.81FIKK501 pKa = 10.85SSTPIEE507 pKa = 4.22RR508 pKa = 11.84SLVVPNPTYY517 pKa = 10.34PHH519 pKa = 7.13LLFDD523 pKa = 3.63CWFDD527 pKa = 3.73YY528 pKa = 10.77KK529 pKa = 10.44HH530 pKa = 6.35ARR532 pKa = 11.84PMTTYY537 pKa = 10.53GVPATLGRR545 pKa = 11.84FF546 pKa = 3.54

Molecular weight: 60.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q8U5N3|A0A3Q8U5N3_9VIRU Nonstructural protein OS=Apis mellifera associated microvirus 24 OX=2494752 PE=4 SV=1
PP1 pKa = 7.33AGNRR5 pKa = 11.84IYY7 pKa = 10.7SPKK10 pKa = 10.13NLPPGLVPDD19 pKa = 4.04VFSCRR24 pKa = 11.84KK25 pKa = 9.39CLPCRR30 pKa = 11.84LNNAKK35 pKa = 9.99EE36 pKa = 3.67KK37 pKa = 10.59AIRR40 pKa = 11.84CWHH43 pKa = 5.99EE44 pKa = 4.37SKK46 pKa = 9.16MHH48 pKa = 7.39DD49 pKa = 4.03DD50 pKa = 4.93NIFLTLTYY58 pKa = 10.32SDD60 pKa = 4.12EE61 pKa = 4.31HH62 pKa = 8.25LEE64 pKa = 4.21SPRR67 pKa = 11.84LIYY70 pKa = 9.35SHH72 pKa = 5.37WQEE75 pKa = 4.91FIHH78 pKa = 7.04KK79 pKa = 9.48LRR81 pKa = 11.84DD82 pKa = 3.46KK83 pKa = 10.73IRR85 pKa = 11.84YY86 pKa = 8.73NSLGEE91 pKa = 4.07QKK93 pKa = 10.14ISTMVTGEE101 pKa = 3.92YY102 pKa = 10.85GEE104 pKa = 4.26QTKK107 pKa = 10.24RR108 pKa = 11.84PHH110 pKa = 4.12WHH112 pKa = 6.62ALIFNYY118 pKa = 10.17RR119 pKa = 11.84PGDD122 pKa = 3.5EE123 pKa = 4.01RR124 pKa = 11.84PYY126 pKa = 10.01RR127 pKa = 11.84TTEE130 pKa = 3.94RR131 pKa = 11.84GDD133 pKa = 3.13KK134 pKa = 10.23VYY136 pKa = 10.6RR137 pKa = 11.84SEE139 pKa = 4.91TIQTLWNKK147 pKa = 10.37GYY149 pKa = 10.16IEE151 pKa = 4.56YY152 pKa = 10.99GDD154 pKa = 3.73VTIDD158 pKa = 3.24SANYY162 pKa = 7.17VARR165 pKa = 11.84YY166 pKa = 7.72AAKK169 pKa = 10.32KK170 pKa = 9.26LVHH173 pKa = 6.9GIDD176 pKa = 3.45QAHH179 pKa = 7.67DD180 pKa = 3.31YY181 pKa = 10.95HH182 pKa = 6.95PIHH185 pKa = 6.33RR186 pKa = 11.84TSSKK190 pKa = 10.29HH191 pKa = 6.32AIGKK195 pKa = 9.2RR196 pKa = 11.84WIEE199 pKa = 3.88KK200 pKa = 9.82YY201 pKa = 10.03YY202 pKa = 10.79KK203 pKa = 8.71QTFEE207 pKa = 4.1HH208 pKa = 6.98GYY210 pKa = 9.85VLLPNFQKK218 pKa = 11.12AKK220 pKa = 9.46IPRR223 pKa = 11.84YY224 pKa = 7.54YY225 pKa = 10.61CEE227 pKa = 4.12WVEE230 pKa = 4.09KK231 pKa = 9.73HH232 pKa = 6.6HH233 pKa = 5.73PAIWEE238 pKa = 4.17RR239 pKa = 11.84YY240 pKa = 4.57VTQIRR245 pKa = 11.84PRR247 pKa = 11.84LQEE250 pKa = 3.44QAEE253 pKa = 3.99AAEE256 pKa = 4.47RR257 pKa = 11.84AEE259 pKa = 4.2EE260 pKa = 4.48MIFLTNLINKK270 pKa = 9.39SCGAPHH276 pKa = 6.65PMKK279 pKa = 10.41RR280 pKa = 11.84SQVKK284 pKa = 7.98EE285 pKa = 4.23TILKK289 pKa = 10.61AKK291 pKa = 10.15FKK293 pKa = 10.57QLQEE297 pKa = 4.03RR298 pKa = 11.84LKK300 pKa = 11.11LL301 pKa = 3.72

Molecular weight: 35.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1302

87

546

260.4

29.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.22 ± 0.86

0.845 ± 0.32

5.991 ± 0.904

5.76 ± 0.832

4.916 ± 1.013

5.223 ± 0.666

2.381 ± 0.799

5.069 ± 0.772

5.914 ± 1.651

7.143 ± 0.241

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.843 ± 0.281

5.223 ± 0.512

6.375 ± 0.612

4.916 ± 0.791

6.298 ± 0.487

7.45 ± 1.106

6.682 ± 0.623

5.146 ± 0.642

1.459 ± 0.269

4.147 ± 0.857
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski