Methylobacterium haplocladii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Methylobacterium

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4347 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A512IVU1|A0A512IVU1_9RHIZ HcpA family protein OS=Methylobacterium haplocladii OX=1176176 GN=MHA02_41830 PE=4 SV=1
MM1 pKa = 7.06VLAGRR6 pKa = 11.84IWGTVMATAYY16 pKa = 7.34NTSVSDD22 pKa = 4.59LDD24 pKa = 3.54QHH26 pKa = 6.97PIDD29 pKa = 3.85PTTRR33 pKa = 11.84AEE35 pKa = 4.01IAQALALSQKK45 pKa = 9.04TGSDD49 pKa = 3.16TVTIEE54 pKa = 4.27APSTDD59 pKa = 2.95GTYY62 pKa = 10.35TLASNTNVFAATVSGSYY79 pKa = 9.21TLDD82 pKa = 3.21TSQSAGVPIIATGADD97 pKa = 3.65PVVLTLTGGVAADD110 pKa = 3.91VIVGSGGAYY119 pKa = 7.71VTGGTGNDD127 pKa = 3.95TIQGGVGSDD136 pKa = 3.43VLFSGYY142 pKa = 10.88AGGDD146 pKa = 3.39HH147 pKa = 6.99KK148 pKa = 10.94ISSGGGNDD156 pKa = 3.27SLYY159 pKa = 11.04GGTGADD165 pKa = 3.5TLTGGMGPGAGSNVLVGGTGKK186 pKa = 9.92NVLQGGDD193 pKa = 4.18GNDD196 pKa = 3.23SLYY199 pKa = 11.34GGGQSLLLAGSGKK212 pKa = 8.02QTLGGGYY219 pKa = 9.47YY220 pKa = 8.06QTAHH224 pKa = 6.58DD225 pKa = 4.17TLVGGTNNDD234 pKa = 3.28LLITFRR240 pKa = 11.84GNNTLSTGNGFGSDD254 pKa = 3.65TLIGGAGNDD263 pKa = 3.44TLTAVHH269 pKa = 6.6GNNVLFGGSTPDD281 pKa = 3.44ADD283 pKa = 3.88GKK285 pKa = 11.13LGISVLQGGDD295 pKa = 3.51GADD298 pKa = 3.14TLYY301 pKa = 11.32GGGRR305 pKa = 11.84SDD307 pKa = 4.88LRR309 pKa = 11.84AGSGNQTLGAGYY321 pKa = 9.53YY322 pKa = 11.0ANAQDD327 pKa = 4.12TLTGGSGNDD336 pKa = 3.84LLITFNGNNTLTTGSDD352 pKa = 3.49GSSDD356 pKa = 3.71TLIGGAGNDD365 pKa = 3.44TLTAVHH371 pKa = 6.63GNNVLVGGSGRR382 pKa = 11.84TLVFGGDD389 pKa = 3.64GNDD392 pKa = 3.34TLFGGGQSDD401 pKa = 3.95LRR403 pKa = 11.84AGTGNQTLGGGYY415 pKa = 9.07IDD417 pKa = 4.5NAHH420 pKa = 6.68DD421 pKa = 3.94TLTGGAGNDD430 pKa = 3.57LLITFKK436 pKa = 11.21GNNSLSTGTGSGHH449 pKa = 7.32DD450 pKa = 3.95SLFGGAGNDD459 pKa = 2.95TLTAVRR465 pKa = 11.84GDD467 pKa = 3.61NLLVAGSGKK476 pKa = 10.11SVLYY480 pKa = 10.78GGTGNDD486 pKa = 3.27TLYY489 pKa = 11.35GSGQSEE495 pKa = 4.02LHH497 pKa = 6.5AGSGSQTLGGGYY509 pKa = 9.64SAGAHH514 pKa = 5.52DD515 pKa = 4.34TLYY518 pKa = 11.03GGSGNSLLVVGQGNNQLTGGSGHH541 pKa = 7.41DD542 pKa = 3.58SLFGGSGNDD551 pKa = 3.55TLSAGANGSVLVAGTGNTLMQGSTGVDD578 pKa = 3.11QFGVSSLTGNDD589 pKa = 3.6TIVGGRR595 pKa = 11.84EE596 pKa = 3.89DD597 pKa = 3.31TLGIYY602 pKa = 10.13NHH604 pKa = 7.28DD605 pKa = 3.89SSAARR610 pKa = 11.84YY611 pKa = 5.74TQNSNGSTTITFEE624 pKa = 3.93GVANHH629 pKa = 6.86SITVSGLGSVTYY641 pKa = 11.02GDD643 pKa = 3.86GTTHH647 pKa = 7.56IFTGGNNSS655 pKa = 3.2

Molecular weight: 63.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A512IR11|A0A512IR11_9RHIZ Molybdenum cofactor sulfurase OS=Methylobacterium haplocladii OX=1176176 GN=MHA02_25420 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATAGGRR28 pKa = 11.84KK29 pKa = 9.15VIAARR34 pKa = 11.84RR35 pKa = 11.84AHH37 pKa = 5.62GRR39 pKa = 11.84KK40 pKa = 9.35KK41 pKa = 10.66LSAA44 pKa = 3.99

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4347

0

4347

1297323

40

4283

298.4

32.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.543 ± 0.047

0.823 ± 0.012

5.744 ± 0.035

5.536 ± 0.04

3.463 ± 0.022

9.032 ± 0.057

1.914 ± 0.021

4.665 ± 0.023

2.908 ± 0.034

10.148 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.096 ± 0.017

2.213 ± 0.029

5.641 ± 0.038

2.782 ± 0.022

7.914 ± 0.046

5.299 ± 0.031

5.482 ± 0.052

7.537 ± 0.029

1.216 ± 0.015

2.044 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski