Pyrococcus abyssi virus 1

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified archaeal dsDNA viruses

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5Y2D8|A5Y2D8_9VIRU Membrane protein OS=Pyrococcus abyssi virus 1 OX=425386 GN=ORF138 PE=4 SV=1
MM1 pKa = 7.23TRR3 pKa = 11.84RR4 pKa = 11.84LSALLIIILAGLLAPQVKK22 pKa = 9.87AATTSYY28 pKa = 11.15YY29 pKa = 10.66GVQFDD34 pKa = 4.51GVDD37 pKa = 3.72DD38 pKa = 3.93YY39 pKa = 12.35VSFNSVTLDD48 pKa = 3.47ANASTVIIFFRR59 pKa = 11.84VNAFSDD65 pKa = 3.42SAYY68 pKa = 10.63GEE70 pKa = 4.09RR71 pKa = 11.84ANIISKK77 pKa = 10.78DD78 pKa = 3.33SGKK81 pKa = 9.65YY82 pKa = 8.8NSYY85 pKa = 10.71IGFTSSGIYY94 pKa = 10.5AEE96 pKa = 4.9GNTNGDD102 pKa = 2.71YY103 pKa = 10.02WAIYY107 pKa = 9.12PYY109 pKa = 10.16TLNLGKK115 pKa = 9.02TYY117 pKa = 10.29MLALVADD124 pKa = 4.59SEE126 pKa = 4.75QVTTYY131 pKa = 11.51LDD133 pKa = 3.52TTKK136 pKa = 10.94LGTRR140 pKa = 11.84PVLSNMTVNALGGAYY155 pKa = 9.97AGSDD159 pKa = 3.09NFFDD163 pKa = 3.82GKK165 pKa = 10.52IYY167 pKa = 10.8LVLIYY172 pKa = 10.64NRR174 pKa = 11.84ALSEE178 pKa = 4.29SEE180 pKa = 3.48IQAIYY185 pKa = 9.95EE186 pKa = 4.42DD187 pKa = 4.56PSNPPTSGLVLWYY200 pKa = 10.39APDD203 pKa = 3.75SVDD206 pKa = 3.16PSTNTWHH213 pKa = 7.32DD214 pKa = 3.26KK215 pKa = 10.81SGNGHH220 pKa = 7.06DD221 pKa = 3.74GSIYY225 pKa = 9.69GATYY229 pKa = 10.49VPLRR233 pKa = 11.84PISEE237 pKa = 4.58SEE239 pKa = 3.77FSDD242 pKa = 3.74AYY244 pKa = 10.5GVAFGSGDD252 pKa = 3.61YY253 pKa = 10.64VVASNVVTGKK263 pKa = 9.99QLTAIVFASVDD274 pKa = 3.24KK275 pKa = 10.7FYY277 pKa = 11.09TPDD280 pKa = 4.01DD281 pKa = 4.39PNKK284 pKa = 10.22GLQPLVSKK292 pKa = 11.05GSDD295 pKa = 2.85RR296 pKa = 11.84FKK298 pKa = 11.11LYY300 pKa = 10.92VGGNYY305 pKa = 10.09ARR307 pKa = 11.84FILVDD312 pKa = 3.3NSLNVYY318 pKa = 10.07RR319 pKa = 11.84LIAGAVEE326 pKa = 4.23NEE328 pKa = 3.84VFMLAGVVDD337 pKa = 3.56NTIAKK342 pKa = 9.75IYY344 pKa = 10.72LNGEE348 pKa = 4.04LKK350 pKa = 9.56EE351 pKa = 4.41TEE353 pKa = 4.26TLSDD357 pKa = 3.91VISDD361 pKa = 4.1YY362 pKa = 11.72VDD364 pKa = 3.07TADD367 pKa = 3.36IFLGKK372 pKa = 10.03RR373 pKa = 11.84SPGDD377 pKa = 3.51YY378 pKa = 10.02QQLDD382 pKa = 3.53GKK384 pKa = 10.38IYY386 pKa = 10.81LVLIYY391 pKa = 10.66NRR393 pKa = 11.84ALSDD397 pKa = 3.58SEE399 pKa = 3.67IRR401 pKa = 11.84QIYY404 pKa = 7.9EE405 pKa = 3.94NPSNPPLDD413 pKa = 5.2GLILWYY419 pKa = 10.08DD420 pKa = 4.06PYY422 pKa = 11.45SYY424 pKa = 11.19DD425 pKa = 3.45PSTGKK430 pKa = 9.09WLNRR434 pKa = 11.84APIFPTIPLVEE445 pKa = 4.29EE446 pKa = 4.31LDD448 pKa = 3.86GVNYY452 pKa = 9.76GAEE455 pKa = 4.18AVRR458 pKa = 11.84VSIPAVKK465 pKa = 10.22AYY467 pKa = 10.01DD468 pKa = 3.45ATDD471 pKa = 3.24NSEE474 pKa = 3.56ISVYY478 pKa = 11.05NLTIKK483 pKa = 10.76SIDD486 pKa = 3.66GEE488 pKa = 4.67TFIPWFMPLPYY499 pKa = 10.48NQTLTLNVTAPGYY512 pKa = 9.85KK513 pKa = 9.61SAIISVQAPIDD524 pKa = 3.52NLVFYY529 pKa = 9.07LYY531 pKa = 9.35PSQVITSWEE540 pKa = 3.91SAQININLNNITADD554 pKa = 3.34EE555 pKa = 4.37FLPDD559 pKa = 3.9ASQDD563 pKa = 2.89IDD565 pKa = 3.69KK566 pKa = 10.71FSSLFTRR573 pKa = 11.84WLGGEE578 pKa = 4.21YY579 pKa = 10.08FKK581 pKa = 11.25HH582 pKa = 6.23LLSLDD587 pKa = 3.82LEE589 pKa = 4.7GMLKK593 pKa = 10.64DD594 pKa = 4.42FFNNPPIPGLGILVALMIWAASVLMTWLYY623 pKa = 10.8FEE625 pKa = 4.66EE626 pKa = 4.86PEE628 pKa = 4.2PVFVEE633 pKa = 4.17GNIVYY638 pKa = 10.16FGLMQFIDD646 pKa = 4.02VSPGILAVPLGVFILFLGYY665 pKa = 9.75KK666 pKa = 9.32QLYY669 pKa = 8.97KK670 pKa = 10.72ALKK673 pKa = 8.52VWAKK677 pKa = 10.18RR678 pKa = 3.54

Molecular weight: 74.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5Y2D0|A5Y2D0_9VIRU Membrane protein OS=Pyrococcus abyssi virus 1 OX=425386 GN=ORF121 PE=4 SV=1
MM1 pKa = 8.38DD2 pKa = 5.53IPPLLCGGQGPIVGPTRR19 pKa = 11.84KK20 pKa = 9.59QYY22 pKa = 8.35ATKK25 pKa = 10.03IFKK28 pKa = 10.38HH29 pKa = 6.02FSSMGNNYY37 pKa = 9.6HH38 pKa = 6.62LRR40 pKa = 11.84EE41 pKa = 3.9KK42 pKa = 10.53KK43 pKa = 9.8RR44 pKa = 11.84YY45 pKa = 8.12KK46 pKa = 10.37GKK48 pKa = 10.47SRR50 pKa = 11.84EE51 pKa = 4.22TQNTPSSSRR60 pKa = 11.84KK61 pKa = 8.55PNQSAYY67 pKa = 7.7ITKK70 pKa = 10.32HH71 pKa = 5.92KK72 pKa = 8.48NTKK75 pKa = 10.09RR76 pKa = 11.84KK77 pKa = 9.55ILGVNN82 pKa = 3.57

Molecular weight: 9.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25

0

25

5787

52

898

231.5

26.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.757 ± 0.364

0.156 ± 0.064

5.754 ± 0.713

6.722 ± 0.781

4.942 ± 0.368

6.29 ± 0.5

1.002 ± 0.178

7.223 ± 0.299

7.189 ± 0.789

11.007 ± 0.648

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.488 ± 0.357

3.819 ± 0.304

3.819 ± 0.398

2.989 ± 0.23

5.149 ± 0.589

5.772 ± 0.496

5.132 ± 0.602

7.482 ± 0.417

1.728 ± 0.203

4.579 ± 0.506
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski