Acetobacter sp. CAG:977

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Acetobacter; environmental samples

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1775 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5QL60|R5QL60_9PROT Glutamate dehydrogenase OS=Acetobacter sp. CAG:977 OX=1262685 GN=BN820_00561 PE=4 SV=1
MM1 pKa = 7.08TMEE4 pKa = 5.61EE5 pKa = 3.94ILKK8 pKa = 8.92TLSEE12 pKa = 5.02DD13 pKa = 3.2NGAMIVRR20 pKa = 11.84PASSKK25 pKa = 11.12DD26 pKa = 3.25LAQCQKK32 pKa = 11.37DD33 pKa = 3.36MAEE36 pKa = 4.28IEE38 pKa = 4.29MHH40 pKa = 6.72PVPQGYY46 pKa = 9.29IDD48 pKa = 4.62FLRR51 pKa = 11.84EE52 pKa = 3.78VNGFAWNGIEE62 pKa = 5.79FFSTDD67 pKa = 2.9QVTDD71 pKa = 3.67PEE73 pKa = 4.69SNYY76 pKa = 9.95TLLDD80 pKa = 3.09IVTANEE86 pKa = 4.49DD87 pKa = 3.28FAEE90 pKa = 4.42YY91 pKa = 10.93NDD93 pKa = 4.92DD94 pKa = 5.72LPDD97 pKa = 4.04CVLLGRR103 pKa = 11.84ADD105 pKa = 4.17DD106 pKa = 5.11DD107 pKa = 4.72LYY109 pKa = 11.77VYY111 pKa = 9.18NTANSKK117 pKa = 11.17YY118 pKa = 9.92EE119 pKa = 3.8VLDD122 pKa = 3.79FTGHH126 pKa = 7.18DD127 pKa = 3.47VMEE130 pKa = 5.74DD131 pKa = 3.33YY132 pKa = 9.82DD133 pKa = 3.91TFDD136 pKa = 3.87ALFEE140 pKa = 4.46NVVSPRR146 pKa = 11.84MITGDD151 pKa = 3.55EE152 pKa = 4.07EE153 pKa = 4.13

Molecular weight: 17.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5PZ18|R5PZ18_9PROT HNH endonuclease domain protein OS=Acetobacter sp. CAG:977 OX=1262685 GN=BN820_00048 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.09QPSKK9 pKa = 8.84IVRR12 pKa = 11.84TRR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.7GFRR19 pKa = 11.84TRR21 pKa = 11.84MATVGGRR28 pKa = 11.84RR29 pKa = 11.84VLNARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.72GRR39 pKa = 11.84KK40 pKa = 9.08RR41 pKa = 11.84LTLGG45 pKa = 3.22

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1775

0

1775

580122

30

2438

326.8

36.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.287 ± 0.059

1.265 ± 0.028

5.678 ± 0.041

6.944 ± 0.062

4.617 ± 0.056

6.863 ± 0.071

1.537 ± 0.023

5.684 ± 0.045

6.893 ± 0.066

9.529 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.695 ± 0.026

3.981 ± 0.041

4.454 ± 0.042

3.028 ± 0.033

5.311 ± 0.043

6.312 ± 0.049

5.103 ± 0.038

6.864 ± 0.048

0.938 ± 0.02

3.01 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski