Pseudomonas sp. 21C1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; unclassified Pseudomonas

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4646 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E4V5H7|A0A1E4V5H7_9PSED Uncharacterized protein OS=Pseudomonas sp. 21C1 OX=1843690 GN=A7D25_00750 PE=4 SV=1
MM1 pKa = 7.58PSEE4 pKa = 5.02HH5 pKa = 6.76SLQHH9 pKa = 4.81LHH11 pKa = 6.78ARR13 pKa = 11.84AALLADD19 pKa = 4.59PQAQDD24 pKa = 3.4CTLYY28 pKa = 10.83RR29 pKa = 11.84PDD31 pKa = 3.68EE32 pKa = 4.14QDD34 pKa = 3.38PEE36 pKa = 4.29AEE38 pKa = 4.18EE39 pKa = 4.63VDD41 pKa = 3.97LGDD44 pKa = 3.82AKK46 pKa = 11.23VVFTGTFEE54 pKa = 4.95PPQDD58 pKa = 2.85WDD60 pKa = 3.48APTRR64 pKa = 11.84EE65 pKa = 4.56AYY67 pKa = 10.71YY68 pKa = 11.03DD69 pKa = 4.0DD70 pKa = 5.99LDD72 pKa = 4.14PALFVIAYY80 pKa = 8.87VEE82 pKa = 4.43SEE84 pKa = 4.06AAPGNRR90 pKa = 11.84GYY92 pKa = 10.88FEE94 pKa = 4.4PAEE97 pKa = 4.29GDD99 pKa = 3.68YY100 pKa = 11.21LAAQPGLGEE109 pKa = 3.92VVMYY113 pKa = 9.35YY114 pKa = 10.54VSEE117 pKa = 4.3CEE119 pKa = 3.52RR120 pKa = 11.84DD121 pKa = 3.49EE122 pKa = 4.88RR123 pKa = 11.84GLRR126 pKa = 11.84CVLIRR131 pKa = 11.84DD132 pKa = 3.98EE133 pKa = 4.9EE134 pKa = 4.14EE135 pKa = 3.86LDD137 pKa = 3.5

Molecular weight: 15.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E4UVH6|A0A1E4UVH6_9PSED AsmA domain-containing protein OS=Pseudomonas sp. 21C1 OX=1843690 GN=A7D25_18115 PE=4 SV=1
MM1 pKa = 7.23VFALYY6 pKa = 9.89IVLGAAAGVLAGLFGVGGGMIIVPALIFTFTAQGFDD42 pKa = 3.62TAVLTHH48 pKa = 6.02MAVGTSLATILFTSINAVRR67 pKa = 11.84EE68 pKa = 4.27HH69 pKa = 6.49HH70 pKa = 6.39RR71 pKa = 11.84HH72 pKa = 4.93GAVRR76 pKa = 11.84WTIFVWLTLGIVFGAALGAVTAEE99 pKa = 4.43SIDD102 pKa = 3.6GQVLQKK108 pKa = 10.53IIGVFALCIALQMALEE124 pKa = 4.81LKK126 pKa = 10.16PKK128 pKa = 9.55VARR131 pKa = 11.84AVPGKK136 pKa = 9.42PVLSAAGVVIGWASAIIGIGGGSLTVPFLTWRR168 pKa = 11.84GVPMQQAVATSSACGWPIAAAGALSFMWLGWGDD201 pKa = 3.27TRR203 pKa = 11.84LPGWSLGYY211 pKa = 10.55LYY213 pKa = 10.9LPALLGIALTSVFFARR229 pKa = 11.84TGARR233 pKa = 11.84LAHH236 pKa = 6.58KK237 pKa = 10.06LSPRR241 pKa = 11.84LLRR244 pKa = 11.84RR245 pKa = 11.84LFALLLVCVGLNFLLL260 pKa = 4.63

Molecular weight: 27.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4646

0

4646

1532859

29

3782

329.9

36.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.663 ± 0.045

0.992 ± 0.012

5.31 ± 0.033

5.71 ± 0.035

3.516 ± 0.022

7.959 ± 0.039

2.215 ± 0.019

4.616 ± 0.026

3.004 ± 0.036

12.33 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.218 ± 0.02

2.864 ± 0.032

4.775 ± 0.026

4.814 ± 0.028

6.61 ± 0.037

5.77 ± 0.034

4.712 ± 0.039

6.986 ± 0.028

1.425 ± 0.017

2.512 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski