Escherichia phage phi467

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B5FPB3|A0A1B5FPB3_9CAUD Uncharacterized protein OS=Escherichia phage phi467 OX=1774508 PE=4 SV=1
MM1 pKa = 7.25ITPLNILEE9 pKa = 4.21EE10 pKa = 4.04VAAQIKK16 pKa = 10.13EE17 pKa = 4.14NTSMLEE23 pKa = 4.27FIFKK27 pKa = 10.54NSPDD31 pKa = 3.41QGEE34 pKa = 4.17TDD36 pKa = 5.51DD37 pKa = 5.1YY38 pKa = 10.77LCCLIRR44 pKa = 11.84SMNKK48 pKa = 6.75TCEE51 pKa = 3.62MAYY54 pKa = 10.2EE55 pKa = 4.16YY56 pKa = 10.55IEE58 pKa = 4.15TLRR61 pKa = 11.84NEE63 pKa = 4.07

Molecular weight: 7.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B5FP91|A0A1B5FP91_9CAUD Phage tail assembly chaperone OS=Escherichia phage phi467 OX=1774508 PE=4 SV=1
MM1 pKa = 6.78QAGRR5 pKa = 11.84LRR7 pKa = 11.84DD8 pKa = 3.15RR9 pKa = 11.84VVILNATTVRR19 pKa = 11.84SPSGHH24 pKa = 6.35PVEE27 pKa = 4.77TMTEE31 pKa = 4.27GATIWAEE38 pKa = 3.91VKK40 pKa = 10.59GISGRR45 pKa = 11.84EE46 pKa = 3.92RR47 pKa = 11.84ISGGAEE53 pKa = 3.43TAQATVRR60 pKa = 11.84VWMRR64 pKa = 11.84FRR66 pKa = 11.84RR67 pKa = 11.84DD68 pKa = 3.11VTATSCLKK76 pKa = 10.7VLTGAFRR83 pKa = 11.84GAILSIDD90 pKa = 3.95GPPIPDD96 pKa = 3.13ARR98 pKa = 11.84ATRR101 pKa = 11.84LEE103 pKa = 4.1ILCSQKK109 pKa = 11.25GNVV112 pKa = 3.36

Molecular weight: 12.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

11177

31

1079

207.0

23.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.75 ± 0.804

1.297 ± 0.18

5.323 ± 0.283

6.782 ± 0.272

3.677 ± 0.346

6.683 ± 0.397

1.754 ± 0.165

6.021 ± 0.405

6.048 ± 0.323

9.001 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.675 ± 0.164

4.742 ± 0.342

3.275 ± 0.284

4.169 ± 0.336

6.299 ± 0.251

7.014 ± 0.399

5.628 ± 0.256

6.147 ± 0.256

1.494 ± 0.112

3.221 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski