Firmicutes bacterium CAG:238

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2016 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6E2C0|R6E2C0_9FIRM Sporulation stage V protein AE OS=Firmicutes bacterium CAG:238 OX=1263011 GN=BN553_01677 PE=4 SV=1
MM1 pKa = 7.59NKK3 pKa = 9.78RR4 pKa = 11.84EE5 pKa = 4.41LYY7 pKa = 10.61ALLDD11 pKa = 3.52IEE13 pKa = 4.5SPEE16 pKa = 3.72EE17 pKa = 3.79FEE19 pKa = 4.67YY20 pKa = 11.19FEE22 pKa = 4.71NLADD26 pKa = 3.78YY27 pKa = 10.5LEE29 pKa = 5.01CEE31 pKa = 4.3EE32 pKa = 4.52EE33 pKa = 4.49LDD35 pKa = 3.93YY36 pKa = 11.7TDD38 pKa = 4.09VAEE41 pKa = 5.3LFNGVNKK48 pKa = 10.48DD49 pKa = 3.7VLAQLCDD56 pKa = 3.35NYY58 pKa = 10.76FEE60 pKa = 5.16EE61 pKa = 5.12ISGFVPGSQTEE72 pKa = 4.17VFTIFEE78 pKa = 4.54NIRR81 pKa = 11.84RR82 pKa = 11.84ALVGMCRR89 pKa = 11.84NCSDD93 pKa = 5.19DD94 pKa = 4.35EE95 pKa = 4.17NLEE98 pKa = 4.13TKK100 pKa = 10.55LIEE103 pKa = 3.92EE104 pKa = 4.68LEE106 pKa = 4.2RR107 pKa = 11.84FRR109 pKa = 11.84RR110 pKa = 11.84WYY112 pKa = 10.81SIDD115 pKa = 3.35SEE117 pKa = 4.88AYY119 pKa = 7.32CTNLGTMQEE128 pKa = 4.14TRR130 pKa = 11.84MPLRR134 pKa = 11.84DD135 pKa = 4.29AIVTSRR141 pKa = 11.84VEE143 pKa = 4.69GIDD146 pKa = 3.5GNTNKK151 pKa = 10.19YY152 pKa = 10.08EE153 pKa = 4.1YY154 pKa = 10.88DD155 pKa = 3.28FSEE158 pKa = 4.4CMNYY162 pKa = 10.07PLDD165 pKa = 4.02EE166 pKa = 4.89YY167 pKa = 10.65IVSLGDD173 pKa = 3.61MIAMGEE179 pKa = 4.13EE180 pKa = 4.22EE181 pKa = 4.9EE182 pKa = 4.25EE183 pKa = 4.69TEE185 pKa = 5.21NSTDD189 pKa = 3.75DD190 pKa = 3.4

Molecular weight: 22.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6E2I4|R6E2I4_9FIRM Pyridine nucleotide-disulphide oxidoreductase family protein OS=Firmicutes bacterium CAG:238 OX=1263011 GN=BN553_00231 PE=4 SV=1
MM1 pKa = 8.02DD2 pKa = 4.37HH3 pKa = 6.54RR4 pKa = 11.84TRR6 pKa = 11.84MNRR9 pKa = 11.84IRR11 pKa = 11.84SLTAVYY17 pKa = 10.27RR18 pKa = 11.84SRR20 pKa = 11.84HH21 pKa = 4.77EE22 pKa = 3.94NRR24 pKa = 11.84VIAALSCMCAVLLGGLGLILSGKK47 pKa = 8.3MSPGLFMVQQNYY59 pKa = 9.45GAVLLRR65 pKa = 11.84GSQKK69 pKa = 10.29PYY71 pKa = 10.75FVIALLAFLAGVTFTLICIKK91 pKa = 10.29INQKK95 pKa = 7.18TRR97 pKa = 11.84RR98 pKa = 11.84LRR100 pKa = 11.84EE101 pKa = 4.02EE102 pKa = 3.7EE103 pKa = 4.04TKK105 pKa = 10.66EE106 pKa = 4.38CEE108 pKa = 4.01RR109 pKa = 11.84QNASNRR115 pKa = 11.84ATQQ118 pKa = 3.05

Molecular weight: 13.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2016

0

2016

603940

30

2015

299.6

33.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.289 ± 0.062

1.549 ± 0.025

5.635 ± 0.041

7.711 ± 0.061

4.135 ± 0.039

7.369 ± 0.047

1.59 ± 0.024

7.587 ± 0.051

7.22 ± 0.053

8.708 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.056 ± 0.031

4.224 ± 0.036

3.331 ± 0.03

2.766 ± 0.03

4.407 ± 0.051

5.618 ± 0.046

5.428 ± 0.048

6.665 ± 0.043

0.814 ± 0.016

3.895 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski