Spiroplasma mirum ATCC 29335

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Spiroplasmataceae; Spiroplasma; Spiroplasma mirum

Average proteome isoelectric point is 7.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1384 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W0GPX9|W0GPX9_9MOLU Uncharacterized protein OS=Spiroplasma mirum ATCC 29335 OX=838561 GN=P344_04315 PE=4 SV=1
MM1 pKa = 8.04DD2 pKa = 4.6YY3 pKa = 10.66DD4 pKa = 3.51YY5 pKa = 11.49SYY7 pKa = 11.86AFKK10 pKa = 10.56TRR12 pKa = 11.84DD13 pKa = 3.35TNKK16 pKa = 10.46ANLQSFTLLFALSDD30 pKa = 3.45SDD32 pKa = 5.37NYY34 pKa = 10.81VSYY37 pKa = 11.67NDD39 pKa = 3.81LQDD42 pKa = 3.64NVFSFPTLSIGGDD55 pKa = 3.25DD56 pKa = 4.49SAFCEE61 pKa = 4.36LL62 pKa = 4.44

Molecular weight: 7.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W0GNZ7|W0GNZ7_9MOLU Uncharacterized protein OS=Spiroplasma mirum ATCC 29335 OX=838561 GN=P344_02010 PE=4 SV=1
MM1 pKa = 7.61AKK3 pKa = 10.14KK4 pKa = 10.19SLKK7 pKa = 10.04VKK9 pKa = 8.54QARR12 pKa = 11.84HH13 pKa = 5.62PKK15 pKa = 9.52FKK17 pKa = 10.49VRR19 pKa = 11.84GYY21 pKa = 7.69TRR23 pKa = 11.84CGNCGRR29 pKa = 11.84PHH31 pKa = 6.52AVLRR35 pKa = 11.84KK36 pKa = 9.43YY37 pKa = 10.28NLCRR41 pKa = 11.84LCFRR45 pKa = 11.84DD46 pKa = 3.97LAYY49 pKa = 9.88KK50 pKa = 10.15GQIPGVRR57 pKa = 11.84KK58 pKa = 10.13ASWW61 pKa = 2.78

Molecular weight: 7.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1384

0

1384

305154

29

1566

220.5

25.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.629 ± 0.073

0.82 ± 0.023

5.12 ± 0.053

5.654 ± 0.088

4.93 ± 0.068

5.115 ± 0.069

1.799 ± 0.034

9.703 ± 0.076

8.539 ± 0.079

10.113 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.271 ± 0.029

7.413 ± 0.091

3.176 ± 0.05

3.979 ± 0.043

2.961 ± 0.048

5.523 ± 0.049

5.694 ± 0.054

6.06 ± 0.059

1.172 ± 0.031

4.327 ± 0.061
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski