Grapevine virus K

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Trivirinae; Vitivirus; unclassified Vitivirus

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A240G1F3|A0A240G1F3_9VIRU RNA-dependent RNA polymerase OS=Grapevine virus K OX=2016034 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.47GDD3 pKa = 3.15EE4 pKa = 4.18VKK6 pKa = 10.64ALKK9 pKa = 10.18VKK11 pKa = 10.75KK12 pKa = 9.38STTNAKK18 pKa = 9.94DD19 pKa = 3.27LQAQIHH25 pKa = 5.9RR26 pKa = 11.84ADD28 pKa = 3.85VYY30 pKa = 11.58DD31 pKa = 3.89EE32 pKa = 4.58GLLEE36 pKa = 4.09RR37 pKa = 11.84LFPKK41 pKa = 9.9RR42 pKa = 11.84TRR44 pKa = 11.84KK45 pKa = 9.95CVVHH49 pKa = 6.91RR50 pKa = 11.84DD51 pKa = 3.26IVVDD55 pKa = 3.91SGEE58 pKa = 4.12VDD60 pKa = 3.64CVLDD64 pKa = 4.61LMDD67 pKa = 6.5DD68 pKa = 3.72EE69 pKa = 5.79GLDD72 pKa = 3.73GVDD75 pKa = 3.43EE76 pKa = 4.54EE77 pKa = 4.75EE78 pKa = 4.67FPLFHH83 pKa = 6.55MGCLVVAVMPHH94 pKa = 4.58GRR96 pKa = 11.84KK97 pKa = 9.71LNGDD101 pKa = 3.8LQIEE105 pKa = 4.4VHH107 pKa = 6.58DD108 pKa = 4.15CRR110 pKa = 11.84LVEE113 pKa = 4.43GKK115 pKa = 10.51SKK117 pKa = 10.91VGAFKK122 pKa = 10.93CDD124 pKa = 2.82ITKK127 pKa = 10.29QLSAFAEE134 pKa = 4.43FPGYY138 pKa = 10.29FISTQDD144 pKa = 3.37LKK146 pKa = 11.1KK147 pKa = 10.78GYY149 pKa = 9.85SLQLALRR156 pKa = 11.84ATGLDD161 pKa = 3.99FKK163 pKa = 11.24DD164 pKa = 3.57GTHH167 pKa = 6.77PFSVQLLTIGRR178 pKa = 11.84FCGEE182 pKa = 4.28DD183 pKa = 3.35LEE185 pKa = 4.69SRR187 pKa = 11.84CAIGGAGKK195 pKa = 9.84SAYY198 pKa = 9.56QCLLNTTKK206 pKa = 10.68EE207 pKa = 3.96QGDD210 pKa = 4.03NFTPLIPRR218 pKa = 11.84QVDD221 pKa = 2.96IRR223 pKa = 11.84EE224 pKa = 3.98VDD226 pKa = 3.32HH227 pKa = 6.71TLVKK231 pKa = 10.49SDD233 pKa = 3.26VFEE236 pKa = 4.44TIKK239 pKa = 10.93RR240 pKa = 11.84LGLRR244 pKa = 11.84TNGKK248 pKa = 9.12LVAEE252 pKa = 4.68SKK254 pKa = 11.19VEE256 pKa = 4.34GDD258 pKa = 3.75NQDD261 pKa = 2.97AGAGGRR267 pKa = 11.84RR268 pKa = 11.84AGGKK272 pKa = 6.46TT273 pKa = 3.07

Molecular weight: 30.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A240G1F6|A0A240G1F6_9VIRU Nucleic acid binding protein OS=Grapevine virus K OX=2016034 GN=ORF5 PE=4 SV=1
MM1 pKa = 8.37DD2 pKa = 5.98DD3 pKa = 3.72PSFLAGRR10 pKa = 11.84SSYY13 pKa = 10.79AKK15 pKa = 9.81KK16 pKa = 10.32RR17 pKa = 11.84RR18 pKa = 11.84AKK20 pKa = 10.38RR21 pKa = 11.84MNVCKK26 pKa = 10.43CGAIMHH32 pKa = 6.53NNPDD36 pKa = 3.64CRR38 pKa = 11.84KK39 pKa = 9.85KK40 pKa = 10.75AISGHH45 pKa = 5.9KK46 pKa = 8.97IDD48 pKa = 4.28RR49 pKa = 11.84LEE51 pKa = 3.77FVKK54 pKa = 10.5RR55 pKa = 11.84GRR57 pKa = 11.84VTLSGEE63 pKa = 4.18TPVYY67 pKa = 8.5RR68 pKa = 11.84TWVRR72 pKa = 11.84WVEE75 pKa = 3.94CEE77 pKa = 3.82YY78 pKa = 11.02GIVVIPSDD86 pKa = 3.97EE87 pKa = 4.35SDD89 pKa = 3.66GEE91 pKa = 4.19

Molecular weight: 10.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2422

91

1700

484.4

55.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.441 ± 0.662

2.766 ± 0.36

6.936 ± 0.698

7.473 ± 0.247

3.799 ± 0.372

6.317 ± 1.2

2.106 ± 0.24

4.459 ± 0.321

7.184 ± 0.557

9.992 ± 1.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.055 ± 0.59

3.386 ± 0.424

3.22 ± 0.15

2.931 ± 0.553

6.689 ± 0.55

7.184 ± 0.706

4.046 ± 0.407

7.184 ± 0.43

1.115 ± 0.372

3.716 ± 0.659
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski