Mycobacterium phage SkinnyPete

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nclasvirinae; Redivirus; Mycobacterium virus Skinnyp

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142ULC8|A0A142ULC8_9CAUD Uncharacterized protein OS=Mycobacterium phage SkinnyPete OX=1821539 GN=61 PE=4 SV=1
MM1 pKa = 7.41NATDD5 pKa = 5.78DD6 pKa = 3.9GLEE9 pKa = 4.17PLGEE13 pKa = 4.24APEE16 pKa = 4.58CGAVLGILRR25 pKa = 11.84SADD28 pKa = 3.47KK29 pKa = 10.66IAATAQLAARR39 pKa = 11.84QAQALNAIRR48 pKa = 11.84VLHH51 pKa = 5.74AAEE54 pKa = 4.05YY55 pKa = 11.11DD56 pKa = 3.52EE57 pKa = 5.42DD58 pKa = 4.61LEE60 pKa = 5.74RR61 pKa = 11.84NTCYY65 pKa = 10.26EE66 pKa = 4.03CSDD69 pKa = 5.12DD70 pKa = 4.33YY71 pKa = 11.3PCPTIRR77 pKa = 11.84VIEE80 pKa = 4.06EE81 pKa = 3.67AGAA84 pKa = 3.49

Molecular weight: 8.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142ULP0|A0A142ULP0_9CAUD RecT-like ssDNA binding protein OS=Mycobacterium phage SkinnyPete OX=1821539 GN=45 PE=4 SV=1
MM1 pKa = 7.41TGLLIALVALQSVSLLVQLSHH22 pKa = 7.25AGATQRR28 pKa = 11.84LLNLALGGRR37 pKa = 11.84RR38 pKa = 3.5

Molecular weight: 3.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

13935

38

1032

208.0

22.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.278 ± 0.656

1.148 ± 0.144

6.609 ± 0.224

5.856 ± 0.348

2.87 ± 0.172

8.762 ± 0.489

2.131 ± 0.204

4.428 ± 0.181

2.921 ± 0.234

7.851 ± 0.276

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.06 ± 0.157

2.892 ± 0.201

6.05 ± 0.228

3.753 ± 0.212

6.882 ± 0.393

5.253 ± 0.236

6.652 ± 0.269

7.241 ± 0.313

1.916 ± 0.159

2.447 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski