Flavobacterium dankookense

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2803 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R6QAK8|A0A4R6QAK8_9FLAO N-acylneuraminate cytidylyltransferase OS=Flavobacterium dankookense OX=706186 GN=BC748_1463 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.4KK3 pKa = 8.43ITNLCKK9 pKa = 10.45LLILFGITFTYY20 pKa = 10.39AQTARR25 pKa = 11.84VQVIHH30 pKa = 6.62NSPDD34 pKa = 3.11LAAQEE39 pKa = 3.77VDD41 pKa = 3.57VYY43 pKa = 11.36INGTIALDD51 pKa = 3.35NFAFRR56 pKa = 11.84TATPFIDD63 pKa = 4.73LPAHH67 pKa = 6.04APITIDD73 pKa = 3.32VAPASSTSVLEE84 pKa = 4.24TLSTTTTTLTAGEE97 pKa = 4.36TYY99 pKa = 10.13IIVANGIVSPTGYY112 pKa = 10.21SVAPNFALSVYY123 pKa = 10.24SGGRR127 pKa = 11.84EE128 pKa = 4.0GTPVLTEE135 pKa = 3.73TDD137 pKa = 3.46VLVAHH142 pKa = 7.18GSPDD146 pKa = 3.52APTVDD151 pKa = 3.58VVEE154 pKa = 4.47TGVGAGTIVNNLSYY168 pKa = 11.32GSFSNYY174 pKa = 10.31LEE176 pKa = 4.38LTTANYY182 pKa = 10.07ILDD185 pKa = 3.72VRR187 pKa = 11.84DD188 pKa = 3.3EE189 pKa = 4.45TGTVTVARR197 pKa = 11.84YY198 pKa = 7.82SAPLSDD204 pKa = 6.17LFLQSDD210 pKa = 5.24AITVVASGFLNPDD223 pKa = 3.45NNSDD227 pKa = 3.74GPAFGLWVAKK237 pKa = 9.25ATGGALIPLPLYY249 pKa = 10.86NPTAKK254 pKa = 10.45VQVIHH259 pKa = 6.55NSPDD263 pKa = 3.47AIAAEE268 pKa = 3.92VDD270 pKa = 3.77VYY272 pKa = 11.48INGDD276 pKa = 3.55LALDD280 pKa = 3.47NFAFRR285 pKa = 11.84TATPFIDD292 pKa = 4.95LPAEE296 pKa = 3.93TSISIDD302 pKa = 3.16IAPSTSSSSSEE313 pKa = 4.01SIYY316 pKa = 11.36NLTTSLAANEE326 pKa = 4.32TYY328 pKa = 10.21IIVANGIVSPTGYY341 pKa = 10.21SVAPNFALNVFSQGRR356 pKa = 11.84EE357 pKa = 3.74FADD360 pKa = 3.89DD361 pKa = 3.96SNLVDD366 pKa = 3.35VLVNHH371 pKa = 7.29GSPDD375 pKa = 3.55APTVDD380 pKa = 3.58VVEE383 pKa = 4.4TAVPAGTVVDD393 pKa = 4.5NISYY397 pKa = 9.08PQFAGYY403 pKa = 10.58LSLPEE408 pKa = 4.98ADD410 pKa = 3.94FVLDD414 pKa = 3.71VRR416 pKa = 11.84DD417 pKa = 3.65EE418 pKa = 4.39TGATTVASYY427 pKa = 9.23QAPFNTFNYY436 pKa = 9.12AGSAITVLASGFLNPAANSDD456 pKa = 3.81GPAFGLWVAFSNGGPLFEE474 pKa = 5.37LPLVEE479 pKa = 4.53VPQTARR485 pKa = 11.84LQVIHH490 pKa = 6.66NSPDD494 pKa = 3.47AIAAEE499 pKa = 3.88VDD501 pKa = 3.86VYY503 pKa = 12.02VNGTLTLDD511 pKa = 3.2NFAFRR516 pKa = 11.84TATPFIDD523 pKa = 3.95VPAGVALSIDD533 pKa = 3.74VAPGNSTSATEE544 pKa = 3.99SVYY547 pKa = 11.39NLTTTLADD555 pKa = 3.56GEE557 pKa = 4.72TYY559 pKa = 10.24IAIANGIVSPTGYY572 pKa = 10.21SVAPNFALSVFAQGRR587 pKa = 11.84EE588 pKa = 4.18VASDD592 pKa = 3.57PAEE595 pKa = 3.92TDD597 pKa = 3.26VLVNHH602 pKa = 7.3GSPDD606 pKa = 3.55APTVDD611 pKa = 3.58VVEE614 pKa = 4.47TGVGAGTIVNDD625 pKa = 3.39ISYY628 pKa = 8.91PQFAGYY634 pKa = 10.64LEE636 pKa = 4.77LPNLDD641 pKa = 3.35FVLDD645 pKa = 3.71VRR647 pKa = 11.84DD648 pKa = 3.77ATGTTTVARR657 pKa = 11.84YY658 pKa = 7.84SAPLQTLNLDD668 pKa = 3.88GAALTVVASGFLNPAANSDD687 pKa = 3.81GPAFGLWVATAAGGNLIQLPPFVEE711 pKa = 4.4PTARR715 pKa = 11.84LQVIHH720 pKa = 6.66NSPDD724 pKa = 3.47AIAAEE729 pKa = 3.88VDD731 pKa = 3.86VYY733 pKa = 12.02VNGTLTLDD741 pKa = 3.2NFAFRR746 pKa = 11.84TATPFIDD753 pKa = 3.95VPAGVALSIDD763 pKa = 3.74VAPGNSTSATEE774 pKa = 4.12SIYY777 pKa = 11.37NLTTTLADD785 pKa = 3.56GEE787 pKa = 4.72TYY789 pKa = 10.24IAIANGIVSPTGYY802 pKa = 10.21SVAPNFALSVFAQGRR817 pKa = 11.84EE818 pKa = 4.04AASDD822 pKa = 3.65PAEE825 pKa = 3.97TDD827 pKa = 3.26VLVNHH832 pKa = 7.3GSPDD836 pKa = 3.55APTVDD841 pKa = 3.58VVEE844 pKa = 4.47TGVGAGTIVNDD855 pKa = 3.39ISYY858 pKa = 8.91PQFAGYY864 pKa = 10.64LEE866 pKa = 4.77LPNLDD871 pKa = 3.35FVLDD875 pKa = 3.71VRR877 pKa = 11.84DD878 pKa = 3.77ATGTTTVASYY888 pKa = 9.76LAPLQTLNLDD898 pKa = 3.73GAAITVIASGFLNPAANSDD917 pKa = 3.81GPAFGLWVATAAGGNLIQLPPFVEE941 pKa = 4.4PTARR945 pKa = 11.84LQVIHH950 pKa = 6.66NSPDD954 pKa = 3.47AIAAEE959 pKa = 3.88VDD961 pKa = 3.86VYY963 pKa = 12.02VNGTLTLDD971 pKa = 3.2NFAFRR976 pKa = 11.84TATPFIDD983 pKa = 3.95VPAGVALSIDD993 pKa = 3.74VAPGNSTSATEE1004 pKa = 3.99SVYY1007 pKa = 11.39NLTTTLADD1015 pKa = 3.56GEE1017 pKa = 4.72TYY1019 pKa = 10.24IAIANGIVSPTGYY1032 pKa = 10.21SVAPNFALSVFAQGRR1047 pKa = 11.84EE1048 pKa = 4.04AASDD1052 pKa = 3.65PAEE1055 pKa = 3.97TDD1057 pKa = 3.26VLVNHH1062 pKa = 7.3GSPDD1066 pKa = 3.55APTVDD1071 pKa = 3.58VVEE1074 pKa = 4.47TGVGAGTIVNDD1085 pKa = 3.39ISYY1088 pKa = 8.91PQFAGYY1094 pKa = 10.64LEE1096 pKa = 4.77LPNLDD1101 pKa = 3.35FVLDD1105 pKa = 3.71VRR1107 pKa = 11.84DD1108 pKa = 3.77ATGTTTVASYY1118 pKa = 9.76LAPLQTLNLDD1128 pKa = 3.73GAAITVIASGFLNPAVNSDD1147 pKa = 3.45GPAFGLWVATAAGGNLIPLPTAPLSVNEE1175 pKa = 4.55NDD1177 pKa = 3.88LEE1179 pKa = 4.68SIRR1182 pKa = 11.84IYY1184 pKa = 10.38PNPANSIINIDD1195 pKa = 3.53IPFSYY1200 pKa = 10.84DD1201 pKa = 2.96NSKK1204 pKa = 10.13VRR1206 pKa = 11.84LFDD1209 pKa = 3.53VNGRR1213 pKa = 11.84SIKK1216 pKa = 10.41EE1217 pKa = 3.9VTSVNSIDD1225 pKa = 3.66VTGLGSGIYY1234 pKa = 9.03MLSLEE1239 pKa = 4.66IDD1241 pKa = 3.19NTVINKK1247 pKa = 10.12KK1248 pKa = 10.01IVITNN1253 pKa = 3.86

Molecular weight: 129.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R6QCJ7|A0A4R6QCJ7_9FLAO Uncharacterized protein OS=Flavobacterium dankookense OX=706186 GN=BC748_1345 PE=4 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.32KK6 pKa = 9.59RR7 pKa = 11.84KK8 pKa = 7.05RR9 pKa = 11.84HH10 pKa = 5.11KK11 pKa = 10.59VATHH15 pKa = 5.35KK16 pKa = 10.31RR17 pKa = 11.84KK18 pKa = 9.82KK19 pKa = 8.65RR20 pKa = 11.84ARR22 pKa = 11.84ANRR25 pKa = 11.84HH26 pKa = 4.91KK27 pKa = 10.59KK28 pKa = 9.82KK29 pKa = 10.51KK30 pKa = 10.04

Molecular weight: 3.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2803

0

2803

969894

24

2750

346.0

39.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.031 ± 0.053

0.808 ± 0.018

5.284 ± 0.034

6.432 ± 0.057

5.643 ± 0.041

6.12 ± 0.052

1.534 ± 0.021

8.31 ± 0.056

7.966 ± 0.091

8.966 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.105 ± 0.025

6.988 ± 0.056

3.3 ± 0.033

3.404 ± 0.025

2.978 ± 0.029

6.68 ± 0.048

6.253 ± 0.093

6.175 ± 0.033

0.994 ± 0.017

4.029 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski