Streptococcus phage Javan206

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6ATI5|A0A4D6ATI5_9CAUD Antirepressor protein OS=Streptococcus phage Javan206 OX=2548046 GN=Javan206_0018 PE=4 SV=1
MM1 pKa = 7.87KK2 pKa = 10.4YY3 pKa = 10.92GLFGTDD9 pKa = 4.84DD10 pKa = 4.01YY11 pKa = 12.13DD12 pKa = 5.72DD13 pKa = 4.24LLEE16 pKa = 4.29RR17 pKa = 11.84QNNYY21 pKa = 7.82GTLFQEE27 pKa = 4.59DD28 pKa = 5.06DD29 pKa = 3.53EE30 pKa = 6.47DD31 pKa = 3.66EE32 pKa = 4.62TYY34 pKa = 11.31DD35 pKa = 3.32RR36 pKa = 11.84WKK38 pKa = 10.85DD39 pKa = 3.59EE40 pKa = 3.99QLGDD44 pKa = 4.11EE45 pKa = 4.39GWKK48 pKa = 9.81II49 pKa = 3.6

Molecular weight: 5.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6ATV7|A0A4D6ATV7_9CAUD Uncharacterized protein OS=Streptococcus phage Javan206 OX=2548046 GN=Javan206_0046 PE=4 SV=1
MM1 pKa = 7.35FRR3 pKa = 11.84IRR5 pKa = 11.84TKK7 pKa = 11.08ADD9 pKa = 3.05LSGAEE14 pKa = 4.38RR15 pKa = 11.84KK16 pKa = 10.24VSDD19 pKa = 3.85ANVLRR24 pKa = 11.84GKK26 pKa = 10.18RR27 pKa = 11.84ALANQVLMDD36 pKa = 3.8TDD38 pKa = 3.96KK39 pKa = 11.12YY40 pKa = 11.24IPMKK44 pKa = 10.69GGALRR49 pKa = 11.84ASGQIAMGGSAVSWNTVYY67 pKa = 10.96ARR69 pKa = 11.84AQFYY73 pKa = 7.49GTNGIVVFRR82 pKa = 11.84KK83 pKa = 8.49YY84 pKa = 6.45TTPGTGKK91 pKa = 10.22LWYY94 pKa = 9.87DD95 pKa = 3.77KK96 pKa = 11.01SAEE99 pKa = 4.06ANVDD103 pKa = 2.4KK104 pKa = 10.43WKK106 pKa = 10.65RR107 pKa = 11.84VAAKK111 pKa = 10.65GMGFF115 pKa = 3.41

Molecular weight: 12.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

11182

38

1309

223.6

25.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.566 ± 0.95

0.519 ± 0.09

6.707 ± 0.47

6.984 ± 0.628

4.293 ± 0.212

6.51 ± 0.522

1.18 ± 0.15

6.68 ± 0.366

8.093 ± 0.446

7.512 ± 0.289

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.62 ± 0.234

5.965 ± 0.417

2.835 ± 0.159

4.328 ± 0.299

3.774 ± 0.29

6.573 ± 0.43

6.287 ± 0.453

6.269 ± 0.327

1.198 ± 0.162

4.105 ± 0.405
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski