Peptostreptococcus anaerobius CAG:621

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae; Peptostreptococcus; environmental samples

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1713 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5IZ25|R5IZ25_9FIRM Iron hydrogenase 1 OS=Peptostreptococcus anaerobius CAG:621 OX=1263100 GN=BN738_01688 PE=4 SV=1
MM1 pKa = 7.35SKK3 pKa = 10.46EE4 pKa = 3.97LVKK7 pKa = 11.04DD8 pKa = 3.54RR9 pKa = 11.84VVEE12 pKa = 3.84YY13 pKa = 10.45LIQEE17 pKa = 4.42LNVPEE22 pKa = 4.65DD23 pKa = 3.9MIEE26 pKa = 3.77IDD28 pKa = 3.88TPLDD32 pKa = 3.52EE33 pKa = 4.69YY34 pKa = 11.42EE35 pKa = 4.44EE36 pKa = 4.52GVEE39 pKa = 4.45GILDD43 pKa = 3.5ITVVAEE49 pKa = 5.0DD50 pKa = 4.4EE51 pKa = 4.72DD52 pKa = 4.97GCLLPLMVIKK62 pKa = 10.86CIDD65 pKa = 3.82DD66 pKa = 5.43DD67 pKa = 4.31IEE69 pKa = 5.8LDD71 pKa = 3.75QSVVDD76 pKa = 4.18ANIDD80 pKa = 3.69EE81 pKa = 5.23LEE83 pKa = 5.1LIDD86 pKa = 4.58DD87 pKa = 4.08TTHH90 pKa = 5.6VGRR93 pKa = 11.84MILTNGDD100 pKa = 2.79QMMYY104 pKa = 9.9TDD106 pKa = 3.55WNGTEE111 pKa = 3.83VDD113 pKa = 4.31EE114 pKa = 7.14DD115 pKa = 4.05SDD117 pKa = 4.06LPNYY121 pKa = 10.56EE122 pKa = 4.63KK123 pKa = 10.48MLEE126 pKa = 4.12EE127 pKa = 4.07YY128 pKa = 10.5KK129 pKa = 10.3ATEE132 pKa = 4.08KK133 pKa = 10.92EE134 pKa = 4.11YY135 pKa = 10.82LDD137 pKa = 4.06YY138 pKa = 11.15VAEE141 pKa = 4.3HH142 pKa = 7.13PEE144 pKa = 3.88YY145 pKa = 10.62EE146 pKa = 4.77DD147 pKa = 3.51EE148 pKa = 4.98HH149 pKa = 7.24EE150 pKa = 4.28HH151 pKa = 6.8

Molecular weight: 17.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5J349|R5J349_9FIRM tRNA modification GTPase MnmE OS=Peptostreptococcus anaerobius CAG:621 OX=1263100 GN=mnmE PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.67QPKK8 pKa = 8.57KK9 pKa = 7.61RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.25RR14 pKa = 11.84EE15 pKa = 3.66HH16 pKa = 6.29GFRR19 pKa = 11.84KK20 pKa = 9.79RR21 pKa = 11.84MKK23 pKa = 7.96TTNGRR28 pKa = 11.84NVLKK32 pKa = 10.51RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.9GRR39 pKa = 11.84NRR41 pKa = 11.84LTHH44 pKa = 6.18

Molecular weight: 5.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1713

0

1713

543760

30

3518

317.4

35.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.117 ± 0.065

1.074 ± 0.022

6.729 ± 0.062

7.018 ± 0.067

4.012 ± 0.041

6.793 ± 0.046

1.4 ± 0.02

8.859 ± 0.065

8.64 ± 0.067

8.715 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.056 ± 0.037

5.18 ± 0.048

2.911 ± 0.035

2.412 ± 0.027

4.136 ± 0.044

6.503 ± 0.071

4.756 ± 0.056

7.005 ± 0.054

0.594 ± 0.017

4.089 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski