Cobetia sp. UCD-24C

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Cobetia; unclassified Cobetia

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3112 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P7DHW8|A0A0P7DHW8_9GAMM Diaminobutyrate--2-oxoglutarate transaminase OS=Cobetia sp. UCD-24C OX=1716176 GN=AOG28_12575 PE=3 SV=1
MM1 pKa = 7.72AFSQALSGLSAASTNLDD18 pKa = 3.46VIGNNIANSEE28 pKa = 4.4TVGFKK33 pKa = 10.57SSEE36 pKa = 3.91AQFSDD41 pKa = 3.69IYY43 pKa = 11.07AGSQAAGIGVQLASVAQDD61 pKa = 3.5FTSGSLEE68 pKa = 3.99STGRR72 pKa = 11.84DD73 pKa = 2.94LDD75 pKa = 4.06LAISGDD81 pKa = 3.37GFLRR85 pKa = 11.84FSDD88 pKa = 3.37TSGQIVYY95 pKa = 10.52SRR97 pKa = 11.84NGQLNIDD104 pKa = 3.65AEE106 pKa = 4.95GYY108 pKa = 9.99IVTAQGAQLTGYY120 pKa = 9.65SGEE123 pKa = 4.34GVTGEE128 pKa = 4.2PVVLQIPTAPLAAKK142 pKa = 8.27ATQDD146 pKa = 3.66NIALEE151 pKa = 4.49VYY153 pKa = 9.88GAEE156 pKa = 4.12VALNLDD162 pKa = 3.72AADD165 pKa = 4.64LVPATADD172 pKa = 3.75FDD174 pKa = 4.1PTDD177 pKa = 3.46AATYY181 pKa = 9.89NYY183 pKa = 9.66ASAMTVYY190 pKa = 10.86DD191 pKa = 3.75SLGDD195 pKa = 3.63EE196 pKa = 4.33HH197 pKa = 8.38VVTSFYY203 pKa = 11.45AKK205 pKa = 9.85TEE207 pKa = 4.04NNTWNVHH214 pKa = 4.43HH215 pKa = 7.18VMDD218 pKa = 5.44FSSKK222 pKa = 10.1AAEE225 pKa = 3.95LQEE228 pKa = 4.44QIDD231 pKa = 4.26EE232 pKa = 4.56GVADD236 pKa = 4.41PSVDD240 pKa = 4.02FDD242 pKa = 4.05PQTEE246 pKa = 4.53VITFGAGGQLLDD258 pKa = 4.12YY259 pKa = 10.56SQTAQTYY266 pKa = 9.56ALANGASDD274 pKa = 4.11LSFSIDD280 pKa = 3.73FTGSTQYY287 pKa = 11.87ANDD290 pKa = 3.74FEE292 pKa = 5.78VITLSQNGYY301 pKa = 7.98TSGSLVGVSFEE312 pKa = 3.87EE313 pKa = 4.36DD314 pKa = 3.04GRR316 pKa = 11.84VIGSYY321 pKa = 10.9SNEE324 pKa = 3.83LKK326 pKa = 9.1QTLGTIALANFANEE340 pKa = 4.74QGLQPNGDD348 pKa = 4.05NGWVATASSGVPLVGVPGEE367 pKa = 4.32GVFGEE372 pKa = 4.45VQSGVVEE379 pKa = 4.24ASNVDD384 pKa = 3.76LTTEE388 pKa = 3.95LVDD391 pKa = 6.24LIIAQRR397 pKa = 11.84NYY399 pKa = 10.3QSNAQTIKK407 pKa = 10.92VQDD410 pKa = 3.75EE411 pKa = 4.47VQQSVINMRR420 pKa = 3.65

Molecular weight: 43.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P7DGN0|A0A0P7DGN0_9GAMM MFS sugar transporter OS=Cobetia sp. UCD-24C OX=1716176 GN=AOG28_16360 PE=4 SV=1
MM1 pKa = 7.67RR2 pKa = 11.84FANPRR7 pKa = 11.84MSLPGSTLTIILMALTLQAGTRR29 pKa = 11.84MAIHH33 pKa = 6.95TATHH37 pKa = 5.8MNIHH41 pKa = 5.08TAIRR45 pKa = 11.84TATHH49 pKa = 6.45MIIHH53 pKa = 7.23TGTTMITRR61 pKa = 11.84LRR63 pKa = 3.79

Molecular weight: 7.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3112

0

3112

1036695

31

5310

333.1

36.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.385 ± 0.062

0.982 ± 0.014

5.763 ± 0.06

6.303 ± 0.049

3.391 ± 0.027

8.184 ± 0.042

2.419 ± 0.024

4.815 ± 0.036

2.848 ± 0.041

11.389 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.664 ± 0.027

2.606 ± 0.028

4.72 ± 0.03

3.955 ± 0.029

6.616 ± 0.052

6.01 ± 0.037

5.14 ± 0.057

7.083 ± 0.04

1.437 ± 0.017

2.29 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski