Influenza A virus (A/black-headed gull/Sweden/87533/2009(H16N3))

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus; Influenza A virus; H16N3 subtype

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A024DAK9|A0A024DAK9_9INFA Polymerase acidic protein OS=Influenza A virus (A/black-headed gull/Sweden/87533/2009(H16N3)) OX=1476312 GN=PA PE=3 SV=1
MM1 pKa = 7.38SLLTEE6 pKa = 4.12VEE8 pKa = 4.27TPIRR12 pKa = 11.84SGWEE16 pKa = 4.09CRR18 pKa = 11.84CSDD21 pKa = 3.47SSDD24 pKa = 3.72PLVVAASVIGILHH37 pKa = 7.21LILWILDD44 pKa = 3.39RR45 pKa = 11.84LFFKK49 pKa = 10.77CIYY52 pKa = 10.15RR53 pKa = 11.84RR54 pKa = 11.84IKK56 pKa = 10.79YY57 pKa = 7.7EE58 pKa = 4.01LKK60 pKa = 10.36RR61 pKa = 11.84GPSTEE66 pKa = 4.02GVPEE70 pKa = 4.24SMRR73 pKa = 11.84EE74 pKa = 3.92EE75 pKa = 4.1YY76 pKa = 10.41RR77 pKa = 11.84QEE79 pKa = 3.74QQSAVDD85 pKa = 3.3VDD87 pKa = 4.47DD88 pKa = 3.92GHH90 pKa = 6.42FVNIEE95 pKa = 3.83LEE97 pKa = 4.08

Molecular weight: 11.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A024DB77|A0A024DB77_9INFA Protein PB1-F2 OS=Influenza A virus (A/black-headed gull/Sweden/87533/2009(H16N3)) OX=1476312 GN=PB1-F2 PE=3 SV=1
MM1 pKa = 7.13EE2 pKa = 4.41QEE4 pKa = 4.55QGIPWTQSTEE14 pKa = 4.18HH15 pKa = 7.0ISTQRR20 pKa = 11.84RR21 pKa = 11.84EE22 pKa = 4.11NGQQTQRR29 pKa = 11.84LVHH32 pKa = 6.43LSLTRR37 pKa = 11.84LMDD40 pKa = 4.59PYY42 pKa = 10.94QRR44 pKa = 11.84IMSQAGMHH52 pKa = 5.58KK53 pKa = 10.36QIVSWRR59 pKa = 11.84QWLSLKK65 pKa = 10.78NPTQGFLKK73 pKa = 9.34THH75 pKa = 5.09VLKK78 pKa = 10.57RR79 pKa = 11.84WKK81 pKa = 10.36LFSKK85 pKa = 10.93HH86 pKa = 5.22EE87 pKa = 4.09WTNN90 pKa = 3.16

Molecular weight: 10.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

4241

90

759

385.5

43.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.918 ± 0.486

1.651 ± 0.374

4.598 ± 0.264

7.734 ± 0.759

4.079 ± 0.258

6.178 ± 0.437

1.674 ± 0.181

6.366 ± 0.258

5.966 ± 0.477

7.946 ± 0.487

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.032 ± 0.449

5.093 ± 0.633

4.032 ± 0.297

4.079 ± 0.485

7.192 ± 0.626

7.31 ± 0.27

6.39 ± 0.428

5.753 ± 0.533

1.556 ± 0.211

2.452 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski