Ralstonia phage RsoM1USA

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Aresaunavirus

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A509Y305|A0A509Y305_9CAUD Uncharacterized protein OS=Ralstonia phage RsoM1USA OX=2079257 GN=RsoM1USA_42 PE=4 SV=1
MM1 pKa = 7.26ATIDD5 pKa = 4.53LSQLPAPSVVEE16 pKa = 3.93TLEE19 pKa = 3.99YY20 pKa = 10.53EE21 pKa = 4.74AILAEE26 pKa = 4.17RR27 pKa = 11.84KK28 pKa = 9.34AAFVSLYY35 pKa = 10.29PADD38 pKa = 3.54QQDD41 pKa = 3.59AVRR44 pKa = 11.84ATLALEE50 pKa = 4.21SEE52 pKa = 4.77PVTKK56 pKa = 10.68LLQEE60 pKa = 3.73NAYY63 pKa = 10.37RR64 pKa = 11.84EE65 pKa = 4.4LVWRR69 pKa = 11.84QRR71 pKa = 11.84VNDD74 pKa = 3.81AARR77 pKa = 11.84AVMLAFAEE85 pKa = 4.48GDD87 pKa = 3.76DD88 pKa = 4.97LEE90 pKa = 4.32QLAANFDD97 pKa = 3.94VQRR100 pKa = 11.84LTITPADD107 pKa = 4.51DD108 pKa = 3.56STVPPTPAVMEE119 pKa = 4.7GDD121 pKa = 3.37DD122 pKa = 4.08SLRR125 pKa = 11.84EE126 pKa = 3.81RR127 pKa = 11.84AQEE130 pKa = 3.88AFEE133 pKa = 4.36GLSVAGPAKK142 pKa = 10.23AYY144 pKa = 10.28EE145 pKa = 4.25FFARR149 pKa = 11.84SADD152 pKa = 3.27GRR154 pKa = 11.84VADD157 pKa = 4.81ARR159 pKa = 11.84AFSPAGAEE167 pKa = 4.11VVVSVLSHH175 pKa = 6.71LGDD178 pKa = 3.61GTADD182 pKa = 3.4EE183 pKa = 4.81SLLATVRR190 pKa = 11.84AALSDD195 pKa = 3.95DD196 pKa = 3.69DD197 pKa = 4.41TRR199 pKa = 11.84PLADD203 pKa = 3.64RR204 pKa = 11.84LTVQSARR211 pKa = 11.84IVPYY215 pKa = 10.32RR216 pKa = 11.84IRR218 pKa = 11.84ATLYY222 pKa = 9.64LAPGPAAEE230 pKa = 5.67PILAAAGKK238 pKa = 8.6RR239 pKa = 11.84TDD241 pKa = 3.07TYY243 pKa = 8.27RR244 pKa = 11.84TARR247 pKa = 11.84RR248 pKa = 11.84RR249 pKa = 11.84IGRR252 pKa = 11.84DD253 pKa = 2.81INRR256 pKa = 11.84SAITAALHH264 pKa = 5.27VEE266 pKa = 4.26GVEE269 pKa = 3.93KK270 pKa = 10.91VVLIEE275 pKa = 3.99PAEE278 pKa = 4.75DD279 pKa = 3.21IALDD283 pKa = 4.16LAQAGYY289 pKa = 8.46CTDD292 pKa = 3.22VDD294 pKa = 3.85IKK296 pKa = 11.44NGGTSEE302 pKa = 4.0

Molecular weight: 32.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A509Y2Z9|A0A509Y2Z9_9CAUD Tail protein OS=Ralstonia phage RsoM1USA OX=2079257 GN=RsoM1USA_23 PE=4 SV=1
MM1 pKa = 6.84TTPKK5 pKa = 10.23VPLSRR10 pKa = 11.84APRR13 pKa = 11.84GVLSEE18 pKa = 4.7KK19 pKa = 9.28PVCMRR24 pKa = 11.84LLPEE28 pKa = 4.11EE29 pKa = 4.25RR30 pKa = 11.84QTLEE34 pKa = 3.5RR35 pKa = 11.84MARR38 pKa = 11.84EE39 pKa = 4.31EE40 pKa = 3.94KK41 pKa = 10.57RR42 pKa = 11.84SISSLARR49 pKa = 11.84LVLLAGIPHH58 pKa = 6.47YY59 pKa = 10.57KK60 pKa = 10.29AGTPRR65 pKa = 11.84TPP67 pKa = 3.08

Molecular weight: 7.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

12008

54

927

218.3

23.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.216 ± 0.65

0.908 ± 0.123

5.829 ± 0.247

5.313 ± 0.233

3.148 ± 0.175

7.42 ± 0.282

2.165 ± 0.231

4.081 ± 0.168

3.989 ± 0.3

9.169 ± 0.308

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.224 ± 0.12

2.898 ± 0.168

5.022 ± 0.264

3.956 ± 0.233

7.737 ± 0.351

5.213 ± 0.238

6.088 ± 0.342

7.395 ± 0.179

1.632 ± 0.149

2.598 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski