Methylophilales bacterium HTCC2181

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; unclassified Nitrosomonadales; OM43 clade

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1336 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0P5G1|A0P5G1_9PROT Anti-sigma F factor antagonist OS=Methylophilales bacterium HTCC2181 OX=383631 GN=MB2181_01820 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.1SKK4 pKa = 10.75SIIAALVFTLTNQAYY19 pKa = 10.24ADD21 pKa = 4.56AILFEE26 pKa = 4.62GLALDD31 pKa = 4.14VSTGYY36 pKa = 9.81QQTEE40 pKa = 3.97RR41 pKa = 11.84DD42 pKa = 3.83TEE44 pKa = 4.33STLLRR49 pKa = 11.84NGVSVSTVTASTDD62 pKa = 3.23EE63 pKa = 4.5GSANAALGVSYY74 pKa = 11.12SFALSSNYY82 pKa = 10.14LVGIGFDD89 pKa = 3.92YY90 pKa = 11.33NFTDD94 pKa = 4.39LDD96 pKa = 3.37IGRR99 pKa = 11.84PLIDD103 pKa = 4.05SNPAEE108 pKa = 4.22STVTFEE114 pKa = 4.59LTNVMNLYY122 pKa = 9.96VKK124 pKa = 9.35PQFMISDD131 pKa = 4.22TSQIYY136 pKa = 10.69AKK138 pKa = 10.34LAYY141 pKa = 8.86TRR143 pKa = 11.84ADD145 pKa = 3.7LEE147 pKa = 4.72SNDD150 pKa = 3.85AVVDD154 pKa = 5.05FIGNTEE160 pKa = 3.98PSLDD164 pKa = 3.82GYY166 pKa = 11.16SIGGGFANMINDD178 pKa = 3.54NVSLFIEE185 pKa = 4.28ANYY188 pKa = 10.08FNYY191 pKa = 10.64GEE193 pKa = 4.3EE194 pKa = 4.2DD195 pKa = 2.74TTYY198 pKa = 10.56RR199 pKa = 11.84YY200 pKa = 10.35DD201 pKa = 3.45GSIGGLTSVGVASDD215 pKa = 3.09IDD217 pKa = 4.16GYY219 pKa = 9.08NAKK222 pKa = 10.16IGIAYY227 pKa = 9.29LFF229 pKa = 4.15

Molecular weight: 24.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0P521|A0P521_9PROT Alkyl hydroperoxide reductase C OS=Methylophilales bacterium HTCC2181 OX=383631 GN=MB2181_01120 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.17QPSKK9 pKa = 9.15IKK11 pKa = 10.36RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.98GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.05GRR39 pKa = 11.84ARR41 pKa = 11.84LGII44 pKa = 3.96

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1336

0

1336

410286

22

3954

307.1

34.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.099 ± 0.058

0.926 ± 0.022

5.595 ± 0.053

6.181 ± 0.074

4.561 ± 0.061

6.742 ± 0.118

2.131 ± 0.034

8.638 ± 0.066

7.485 ± 0.082

9.784 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.561 ± 0.033

5.346 ± 0.063

3.674 ± 0.042

3.388 ± 0.032

3.866 ± 0.055

6.96 ± 0.067

4.996 ± 0.073

6.046 ± 0.052

0.975 ± 0.023

3.045 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski