Human respiratory syncytial virus A (strain A2)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Pneumoviridae; Orthopneumovirus; Human orthopneumovirus; Human respiratory syncytial virus A

Average proteome isoelectric point is 7.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P03423|GLYC_HRSVA Major surface glycoprotein G OS=Human respiratory syncytial virus A (strain A2) OX=11259 GN=G PE=1 SV=1
MM1 pKa = 7.59EE2 pKa = 4.71KK3 pKa = 10.15FAPEE7 pKa = 3.71FHH9 pKa = 7.34GEE11 pKa = 4.02DD12 pKa = 3.43ANNRR16 pKa = 11.84ATKK19 pKa = 10.09FLEE22 pKa = 4.38SIKK25 pKa = 11.05GKK27 pKa = 8.62FTSPKK32 pKa = 10.1DD33 pKa = 3.61PKK35 pKa = 10.66KK36 pKa = 9.85KK37 pKa = 10.44DD38 pKa = 3.43SIISVNSIDD47 pKa = 4.07IEE49 pKa = 4.43VTKK52 pKa = 10.47EE53 pKa = 3.86SPITSNSTIINPTNEE68 pKa = 4.09TDD70 pKa = 3.35DD71 pKa = 3.82TAGNKK76 pKa = 9.17PNYY79 pKa = 7.91QRR81 pKa = 11.84KK82 pKa = 8.31PLVSFKK88 pKa = 10.76EE89 pKa = 4.16DD90 pKa = 3.54PTPSDD95 pKa = 3.46NPFSKK100 pKa = 10.44LYY102 pKa = 10.59KK103 pKa = 9.24EE104 pKa = 4.59TIEE107 pKa = 4.06TFDD110 pKa = 3.63NNEE113 pKa = 3.96EE114 pKa = 4.04EE115 pKa = 4.21SSYY118 pKa = 11.27SYY120 pKa = 11.54EE121 pKa = 4.37EE122 pKa = 5.29INDD125 pKa = 3.58QTNDD129 pKa = 3.44NITARR134 pKa = 11.84LDD136 pKa = 4.15RR137 pKa = 11.84IDD139 pKa = 4.16EE140 pKa = 4.3KK141 pKa = 11.17LSEE144 pKa = 4.26ILGMLHH150 pKa = 5.8TLVVASAGPTSARR163 pKa = 11.84DD164 pKa = 3.98GIRR167 pKa = 11.84DD168 pKa = 3.45AMIGLRR174 pKa = 11.84EE175 pKa = 3.94EE176 pKa = 4.38MIEE179 pKa = 4.35KK180 pKa = 10.05IRR182 pKa = 11.84TEE184 pKa = 3.85ALMTNDD190 pKa = 3.88RR191 pKa = 11.84LEE193 pKa = 4.27AMARR197 pKa = 11.84LRR199 pKa = 11.84NEE201 pKa = 4.35EE202 pKa = 4.19SEE204 pKa = 4.95KK205 pKa = 9.88MAKK208 pKa = 8.76DD209 pKa = 3.25TSDD212 pKa = 3.6EE213 pKa = 4.1VSLNPTSEE221 pKa = 4.08KK222 pKa = 10.86LNNLLEE228 pKa = 4.91GNDD231 pKa = 3.63SDD233 pKa = 5.53NDD235 pKa = 3.54LSLEE239 pKa = 4.14DD240 pKa = 4.02FF241 pKa = 4.61

Molecular weight: 27.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P04543|NS2_HRSVA Non-structural protein 2 OS=Human respiratory syncytial virus A (strain A2) OX=11259 GN=1B PE=1 SV=2
MM1 pKa = 7.64SKK3 pKa = 10.75NKK5 pKa = 9.76DD6 pKa = 3.13QRR8 pKa = 11.84TAKK11 pKa = 9.53TLEE14 pKa = 4.28RR15 pKa = 11.84TWDD18 pKa = 3.72TLNHH22 pKa = 6.38LLFISSCLYY31 pKa = 10.5KK32 pKa = 11.03LNLKK36 pKa = 10.06SVAQITLSILAMIISTSLIIAAIIFIASANHH67 pKa = 6.28KK68 pKa = 7.56VTPTTAIIQDD78 pKa = 3.45ATSQIKK84 pKa = 8.15NTTPTYY90 pKa = 9.41LTQNPQLGISPSNPSEE106 pKa = 3.68ITSQITTILASTTPGVKK123 pKa = 8.65STLQSTTVKK132 pKa = 9.77TKK134 pKa = 9.51NTTTTQTQPSKK145 pKa = 9.62PTTKK149 pKa = 9.84QRR151 pKa = 11.84QNKK154 pKa = 8.8PPSKK158 pKa = 9.81PNNDD162 pKa = 2.88FHH164 pKa = 9.4FEE166 pKa = 3.92VFNFVPCSICSNNPTCWAICKK187 pKa = 9.86RR188 pKa = 11.84IPNKK192 pKa = 10.49KK193 pKa = 9.02PGKK196 pKa = 8.86KK197 pKa = 6.63TTTKK201 pKa = 8.85PTKK204 pKa = 10.36KK205 pKa = 8.83PTLKK209 pKa = 8.2TTKK212 pKa = 9.87KK213 pKa = 10.32DD214 pKa = 3.7PKK216 pKa = 10.22PQTTKK221 pKa = 10.63SKK223 pKa = 10.3EE224 pKa = 4.03VPTTKK229 pKa = 9.17PTEE232 pKa = 4.01EE233 pKa = 3.98PTINTTKK240 pKa = 10.59TNIITTLLTSNTTGNPEE257 pKa = 4.04LTSQMEE263 pKa = 4.68TFHH266 pKa = 6.18STSSEE271 pKa = 4.09GNPSPSQVSTTSEE284 pKa = 4.32YY285 pKa = 10.25PSQPSSPPNTPRR297 pKa = 11.84QQ298 pKa = 3.41

Molecular weight: 32.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

3

14

4904

42

2165

350.3

39.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.997 ± 0.543

1.794 ± 0.176

4.527 ± 0.512

5.016 ± 0.41

3.589 ± 0.325

3.67 ± 0.469

2.447 ± 0.384

9.013 ± 0.425

8.075 ± 0.255

10.522 ± 1.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.467 ± 0.253

7.912 ± 0.328

4.18 ± 0.92

3.161 ± 0.413

3.263 ± 0.303

8.218 ± 0.473

8.585 ± 1.737

4.853 ± 0.569

0.836 ± 0.185

3.874 ± 0.621
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski