Mycobacterium phage RhynO

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8ED06|W8ED06_9CAUD Portal protein OS=Mycobacterium phage RhynO OX=1458846 GN=13 PE=4 SV=1
MM1 pKa = 7.32NPDD4 pKa = 3.67DD5 pKa = 5.12EE6 pKa = 4.82YY7 pKa = 11.18TFAIRR12 pKa = 11.84YY13 pKa = 7.52EE14 pKa = 4.45GQQEE18 pKa = 4.1PDD20 pKa = 3.9GEE22 pKa = 4.35WTEE25 pKa = 4.33MVALQPSLAVAQEE38 pKa = 4.46TFASMLPYY46 pKa = 10.19FEE48 pKa = 5.65SLPNVRR54 pKa = 11.84NAAIAYY60 pKa = 5.73TPKK63 pKa = 10.43INWQPWEE70 pKa = 4.05GG71 pKa = 3.46

Molecular weight: 8.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8ED57|W8ED57_9CAUD Uncharacterized protein OS=Mycobacterium phage RhynO OX=1458846 GN=73 PE=4 SV=1
MM1 pKa = 6.87TTMTPVRR8 pKa = 11.84TMTITQARR16 pKa = 11.84EE17 pKa = 3.48ITQDD21 pKa = 3.46LLHH24 pKa = 6.02EE25 pKa = 5.3HH26 pKa = 6.49GLKK29 pKa = 10.3GWVVRR34 pKa = 11.84FDD36 pKa = 3.4NARR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84AGQCSYY47 pKa = 10.92RR48 pKa = 11.84DD49 pKa = 3.44RR50 pKa = 11.84AISLSRR56 pKa = 11.84PLMALRR62 pKa = 11.84PYY64 pKa = 9.86EE65 pKa = 4.2DD66 pKa = 3.24TMQTITHH73 pKa = 6.69EE74 pKa = 4.15LAHH77 pKa = 6.44ALVGPSHH84 pKa = 6.19GHH86 pKa = 4.7DD87 pKa = 3.25HH88 pKa = 4.91VWARR92 pKa = 11.84KK93 pKa = 8.85HH94 pKa = 6.74RR95 pKa = 11.84EE96 pKa = 3.5LGGNGKK102 pKa = 9.46RR103 pKa = 11.84CFEE106 pKa = 4.33MEE108 pKa = 5.16GIDD111 pKa = 4.18PTAPWVGTCAHH122 pKa = 7.03GKK124 pKa = 9.1QFARR128 pKa = 11.84YY129 pKa = 7.47RR130 pKa = 11.84APKK133 pKa = 9.88RR134 pKa = 11.84LEE136 pKa = 3.58GWRR139 pKa = 11.84CRR141 pKa = 11.84CRR143 pKa = 11.84QGSSPVTWEE152 pKa = 3.92KK153 pKa = 11.07KK154 pKa = 8.31RR155 pKa = 3.53

Molecular weight: 17.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

14635

27

851

200.5

22.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.839 ± 0.408

0.875 ± 0.134

6.307 ± 0.184

6.819 ± 0.302

3.116 ± 0.163

8.944 ± 0.494

2.146 ± 0.196

4.585 ± 0.174

4.23 ± 0.283

8.049 ± 0.266

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.269 ± 0.154

3.198 ± 0.184

6.197 ± 0.362

3.621 ± 0.213

6.792 ± 0.364

4.968 ± 0.187

5.61 ± 0.229

7.373 ± 0.189

2.105 ± 0.141

2.959 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski