Changping earthworm virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KKP1|A0A1L3KKP1_9VIRU Uncharacterized protein OS=Changping earthworm virus 2 OX=1922827 PE=4 SV=1
MM1 pKa = 7.52CSDD4 pKa = 3.01VSGRR8 pKa = 11.84LSVEE12 pKa = 3.74LAKK15 pKa = 10.52CRR17 pKa = 11.84QVSFPKK23 pKa = 10.66GVIVTLGPKK32 pKa = 9.93CDD34 pKa = 3.91GSDD37 pKa = 3.54CYY39 pKa = 11.72GEE41 pKa = 4.38FFPLITFTEE50 pKa = 4.23KK51 pKa = 10.62CFPEE55 pKa = 5.69FIRR58 pKa = 11.84DD59 pKa = 3.44WGWALFNVVCNCTTASTPLSISRR82 pKa = 11.84EE83 pKa = 3.91EE84 pKa = 4.09SAKK87 pKa = 10.65SCFISKK93 pKa = 8.35STFLEE98 pKa = 5.11GYY100 pKa = 8.95GRR102 pKa = 11.84YY103 pKa = 9.55GADD106 pKa = 3.44PGWAARR112 pKa = 11.84TLVRR116 pKa = 11.84PKK118 pKa = 9.51FTTTIEE124 pKa = 4.17EE125 pKa = 3.97FEE127 pKa = 4.32EE128 pKa = 4.85WVGHH132 pKa = 5.67IANVAAGNRR141 pKa = 11.84GPAGSQEE148 pKa = 4.0SGEE151 pKa = 4.07AHH153 pKa = 6.98FSNVDD158 pKa = 3.02QWGGRR163 pKa = 11.84IAGTDD168 pKa = 3.5FVVRR172 pKa = 11.84HH173 pKa = 4.9VTSEE177 pKa = 4.22CEE179 pKa = 3.93LEE181 pKa = 4.03RR182 pKa = 11.84GYY184 pKa = 11.42KK185 pKa = 9.55FAGPNKK191 pKa = 10.06DD192 pKa = 3.7LEE194 pKa = 4.09LHH196 pKa = 5.58LRR198 pKa = 11.84YY199 pKa = 9.77RR200 pKa = 11.84PFFHH204 pKa = 7.42IEE206 pKa = 3.45TSTAGTLGVRR216 pKa = 11.84VFGSPIYY223 pKa = 9.45WAMWSYY229 pKa = 10.43FAHH232 pKa = 6.29NPGITGAEE240 pKa = 3.6QHH242 pKa = 6.17IEE244 pKa = 3.94KK245 pKa = 10.57FNLKK249 pKa = 9.95FGEE252 pKa = 4.2AAWIQKK258 pKa = 10.12VGIIYY263 pKa = 10.28KK264 pKa = 10.03DD265 pKa = 3.55VPDD268 pKa = 4.46EE269 pKa = 4.75GDD271 pKa = 3.14VEE273 pKa = 4.4YY274 pKa = 10.84QGEE277 pKa = 4.21DD278 pKa = 3.28EE279 pKa = 4.47EE280 pKa = 5.54EE281 pKa = 4.24YY282 pKa = 10.98DD283 pKa = 3.83GSEE286 pKa = 4.14EE287 pKa = 4.86LEE289 pKa = 4.13EE290 pKa = 5.26LPTLSDD296 pKa = 3.26IEE298 pKa = 4.43VNPEE302 pKa = 3.62EE303 pKa = 3.95IQARR307 pKa = 11.84QEE309 pKa = 4.09SALLEE314 pKa = 4.02MSALKK319 pKa = 10.67DD320 pKa = 3.38SLPRR324 pKa = 11.84AFYY327 pKa = 10.6KK328 pKa = 10.15PLPRR332 pKa = 11.84VQRR335 pKa = 11.84SDD337 pKa = 3.06FLQRR341 pKa = 11.84YY342 pKa = 4.17TAPYY346 pKa = 9.79RR347 pKa = 11.84KK348 pKa = 9.74

Molecular weight: 39.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KKH0|A0A1L3KKH0_9VIRU Uncharacterized protein OS=Changping earthworm virus 2 OX=1922827 PE=4 SV=1
MM1 pKa = 6.91NHH3 pKa = 6.22RR4 pKa = 11.84MNSRR8 pKa = 11.84KK9 pKa = 9.31QKK11 pKa = 10.43HH12 pKa = 5.5GDD14 pKa = 3.02MMNYY18 pKa = 10.41NMTNIYY24 pKa = 8.03NTNAMMRR31 pKa = 11.84IRR33 pKa = 11.84KK34 pKa = 9.19RR35 pKa = 11.84KK36 pKa = 9.63INDD39 pKa = 3.53AEE41 pKa = 4.1DD42 pKa = 3.15MRR44 pKa = 11.84AKK46 pKa = 10.36RR47 pKa = 11.84KK48 pKa = 9.9CLEE51 pKa = 4.24DD52 pKa = 3.37LRR54 pKa = 11.84KK55 pKa = 9.95LRR57 pKa = 11.84TEE59 pKa = 3.63THH61 pKa = 4.78EE62 pKa = 3.92TGFRR66 pKa = 11.84IEE68 pKa = 4.06TLRR71 pKa = 11.84AAEE74 pKa = 4.83LLMEE78 pKa = 5.03DD79 pKa = 4.62AGFSDD84 pKa = 4.02STVTRR89 pKa = 11.84ANSKK93 pKa = 9.8LLRR96 pKa = 11.84NLGHH100 pKa = 7.13KK101 pKa = 9.93IKK103 pKa = 10.44PPNIQLHH110 pKa = 6.05NISMNRR116 pKa = 11.84FSSIMVKK123 pKa = 9.53TSEE126 pKa = 4.39YY127 pKa = 10.09EE128 pKa = 4.05LNSLGIEE135 pKa = 4.59PIHH138 pKa = 6.19AAGRR142 pKa = 11.84TYY144 pKa = 10.79IKK146 pKa = 10.67FQDD149 pKa = 4.02LSKK152 pKa = 10.41LCCSRR157 pKa = 11.84TTDD160 pKa = 3.67FDD162 pKa = 3.76SALAEE167 pKa = 4.3FFNLMSQRR175 pKa = 11.84TQRR178 pKa = 11.84SYY180 pKa = 11.8NLWKK184 pKa = 10.82SLVDD188 pKa = 3.26EE189 pKa = 4.5AQILEE194 pKa = 4.38VPRR197 pKa = 11.84VPTHH201 pKa = 5.25QLQEE205 pKa = 3.78VVMRR209 pKa = 11.84DD210 pKa = 3.03KK211 pKa = 11.21VLPTTSSPLEE221 pKa = 3.82VLSYY225 pKa = 9.9CFPAVYY231 pKa = 9.93PNVVRR236 pKa = 11.84LSPLAMVEE244 pKa = 3.66NDD246 pKa = 3.08AKK248 pKa = 10.74FVEE251 pKa = 4.44NVIRR255 pKa = 11.84MVKK258 pKa = 9.94SLHH261 pKa = 6.21FKK263 pKa = 9.61MLNQIDD269 pKa = 4.45GEE271 pKa = 4.65VLGHH275 pKa = 6.83HH276 pKa = 6.55LMKK279 pKa = 11.08LMTEE283 pKa = 4.35DD284 pKa = 3.46KK285 pKa = 10.94GLRR288 pKa = 11.84NCSRR292 pKa = 11.84ATPEE296 pKa = 3.79GFEE299 pKa = 4.06MFSCFFNNTYY309 pKa = 9.72EE310 pKa = 4.16SHH312 pKa = 6.69SISINMAPPPRR323 pKa = 11.84TDD325 pKa = 4.28KK326 pKa = 11.16RR327 pKa = 11.84IFDD330 pKa = 4.9CILNFAYY337 pKa = 9.7KK338 pKa = 10.19FVDD341 pKa = 4.0GSVTKK346 pKa = 10.36TSLIKK351 pKa = 10.87AFTKK355 pKa = 10.51FADD358 pKa = 3.46THH360 pKa = 6.22RR361 pKa = 11.84FTIKK365 pKa = 10.44DD366 pKa = 4.09HH367 pKa = 6.83DD368 pKa = 3.89WSSYY372 pKa = 10.36GYY374 pKa = 10.4EE375 pKa = 4.24CLFCTALMNGDD386 pKa = 3.74FPKK389 pKa = 10.69VHH391 pKa = 6.6TKK393 pKa = 10.14HH394 pKa = 7.11DD395 pKa = 4.25YY396 pKa = 8.43VTSILKK402 pKa = 10.63AGGILIEE409 pKa = 4.56ILDD412 pKa = 3.74NRR414 pKa = 11.84CAYY417 pKa = 9.3RR418 pKa = 11.84KK419 pKa = 9.9KK420 pKa = 10.45YY421 pKa = 9.44PRR423 pKa = 11.84PKK425 pKa = 10.35KK426 pKa = 9.78RR427 pKa = 11.84FHH429 pKa = 7.0SNRR432 pKa = 11.84LMPGAPVPLYY442 pKa = 9.3PLMPVVHH449 pKa = 6.52TEE451 pKa = 5.22DD452 pKa = 3.03KK453 pKa = 10.34TEE455 pKa = 3.72WVTFLTGEE463 pKa = 4.65KK464 pKa = 9.73MIFHH468 pKa = 7.11NKK470 pKa = 8.0TSQCYY475 pKa = 9.37FEE477 pKa = 6.34IIGLNKK483 pKa = 10.18SPSFEE488 pKa = 4.22ITCSPQVSPDD498 pKa = 3.29CLTRR502 pKa = 11.84CYY504 pKa = 10.87KK505 pKa = 10.48FVQHH509 pKa = 6.32WLGYY513 pKa = 9.53PPGNSGNKK521 pKa = 9.15YY522 pKa = 8.48LWSRR526 pKa = 11.84QFDD529 pKa = 4.09PRR531 pKa = 11.84CCWPFDD537 pKa = 3.62SDD539 pKa = 5.39DD540 pKa = 4.8PYY542 pKa = 10.83TSKK545 pKa = 11.2PKK547 pKa = 8.73MLFVAVHH554 pKa = 6.22NIEE557 pKa = 4.05RR558 pKa = 11.84SQIEE562 pKa = 4.11IQIQPSGKK570 pKa = 9.16VLKK573 pKa = 9.57CLSRR577 pKa = 11.84ITVDD581 pKa = 3.25TKK583 pKa = 10.19VKK585 pKa = 9.85PRR587 pKa = 11.84ARR589 pKa = 11.84SLRR592 pKa = 11.84MQVLDD597 pKa = 4.02FNKK600 pKa = 10.47SGVIGQLVGSGQKK613 pKa = 8.5MQILPMPPPKK623 pKa = 9.8PWPSGFAFEE632 pKa = 4.72GAAGLDD638 pKa = 3.73PNVTFHH644 pKa = 6.91TKK646 pKa = 8.94QQVFKK651 pKa = 10.74QLVSFLLNHH660 pKa = 6.43SMPPDD665 pKa = 3.51VLLSTVLPEE674 pKa = 3.95WKK676 pKa = 8.64STFGRR681 pKa = 11.84LLGGLEE687 pKa = 3.97TLAVRR692 pKa = 11.84SHH694 pKa = 5.98RR695 pKa = 11.84HH696 pKa = 3.56GHH698 pKa = 5.65SYY700 pKa = 10.39NIEE703 pKa = 4.08YY704 pKa = 9.36MSKK707 pKa = 9.09TKK709 pKa = 10.7NISNFLRR716 pKa = 11.84TLALSVPRR724 pKa = 11.84EE725 pKa = 4.09DD726 pKa = 3.32NVTIVKK732 pKa = 9.44VNPCDD737 pKa = 3.5MVLSITDD744 pKa = 5.02DD745 pKa = 3.06ILKK748 pKa = 8.74YY749 pKa = 7.08TTSCPPMEE757 pKa = 4.16VVEE760 pKa = 4.73FVSVTKK766 pKa = 10.34EE767 pKa = 3.56PWALSSISLGPGQCNEE783 pKa = 3.66GMFLILARR791 pKa = 11.84TRR793 pKa = 11.84HH794 pKa = 5.7RR795 pKa = 11.84SVSQLEE801 pKa = 4.1GMGYY805 pKa = 9.98KK806 pKa = 9.68VEE808 pKa = 4.22KK809 pKa = 10.48QLTADD814 pKa = 3.75EE815 pKa = 4.68YY816 pKa = 11.29EE817 pKa = 4.09NLPFYY822 pKa = 9.78RR823 pKa = 11.84TLEE826 pKa = 3.85ILGKK830 pKa = 8.67FTSLGFLPLEE840 pKa = 4.37GMGTSILGIVVKK852 pKa = 10.63EE853 pKa = 4.28DD854 pKa = 3.27VACEE858 pKa = 3.85YY859 pKa = 10.36WVVSLKK865 pKa = 10.83DD866 pKa = 3.47VSTKK870 pKa = 10.22PFQIKK875 pKa = 9.34ARR877 pKa = 11.84KK878 pKa = 8.51GKK880 pKa = 9.99RR881 pKa = 11.84INIGTTTGADD891 pKa = 4.45LINEE895 pKa = 4.77AIEE898 pKa = 4.68KK899 pKa = 9.88IKK901 pKa = 10.55QRR903 pKa = 11.84NLEE906 pKa = 4.29ANKK909 pKa = 9.07STAEE913 pKa = 4.02DD914 pKa = 4.3LYY916 pKa = 11.95KK917 pKa = 10.13MVNDD921 pKa = 4.43LDD923 pKa = 5.01DD924 pKa = 3.95WDD926 pKa = 4.11TDD928 pKa = 4.31SIAGEE933 pKa = 4.11LKK935 pKa = 10.95DD936 pKa = 6.05NDD938 pKa = 4.18MAWLNDD944 pKa = 3.63RR945 pKa = 11.84VSMSDD950 pKa = 2.82RR951 pKa = 11.84ASPTRR956 pKa = 11.84TLSRR960 pKa = 11.84PGTPTSVEE968 pKa = 4.05TATASALSSWDD979 pKa = 3.3FKK981 pKa = 10.98RR982 pKa = 11.84VNRR985 pKa = 11.84RR986 pKa = 11.84LSFF989 pKa = 3.83

Molecular weight: 112.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

4252

244

1292

607.4

68.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.691 ± 0.438

2.117 ± 0.252

4.915 ± 0.19

5.738 ± 0.575

4.586 ± 0.289

5.456 ± 0.642

2.352 ± 0.437

5.691 ± 0.189

5.88 ± 0.504

9.737 ± 0.691

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.658 ± 0.367

4.516 ± 0.403

4.986 ± 0.257

3.504 ± 0.384

5.127 ± 0.407

8.984 ± 0.678

6.985 ± 0.447

6.115 ± 0.481

1.599 ± 0.192

3.293 ± 0.37
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski