Ralstonia phage Dina

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Dinavirus; Ralstonia virus Dina

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G5B9D6|A0A7G5B9D6_9CAUD Uncharacterized protein OS=Ralstonia phage Dina OX=2759732 GN=2CaD_00019 PE=4 SV=1
MM1 pKa = 8.5DD2 pKa = 4.4YY3 pKa = 10.45PIEE6 pKa = 4.77PIDD9 pKa = 4.34AIEE12 pKa = 4.0RR13 pKa = 11.84RR14 pKa = 11.84GRR16 pKa = 11.84SAMCNGLEE24 pKa = 4.68PEE26 pKa = 4.33MCPYY30 pKa = 10.85DD31 pKa = 4.12YY32 pKa = 11.49DD33 pKa = 4.0SAHH36 pKa = 5.11WRR38 pKa = 11.84AWQVGFLAAALEE50 pKa = 4.46VATAAAVCVDD60 pKa = 4.59DD61 pKa = 5.65EE62 pKa = 4.63VAAA65 pKa = 4.51

Molecular weight: 7.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G5B9E8|A0A7G5B9E8_9CAUD Neck protein 1 OS=Ralstonia phage Dina OX=2759732 GN=2CaD_00031 PE=4 SV=1
MM1 pKa = 7.46QRR3 pKa = 11.84GKK5 pKa = 9.78YY6 pKa = 8.51NRR8 pKa = 11.84RR9 pKa = 11.84IVLQRR14 pKa = 11.84RR15 pKa = 11.84EE16 pKa = 4.1AGRR19 pKa = 11.84APSGQPINAWVDD31 pKa = 3.42VARR34 pKa = 11.84PWARR38 pKa = 11.84VRR40 pKa = 11.84GEE42 pKa = 3.79NGKK45 pKa = 9.37EE46 pKa = 3.63FIAADD51 pKa = 4.11RR52 pKa = 11.84EE53 pKa = 4.42TAEE56 pKa = 4.2SDD58 pKa = 3.14VSLRR62 pKa = 11.84IRR64 pKa = 11.84YY65 pKa = 7.4RR66 pKa = 11.84TDD68 pKa = 2.31VTAAWRR74 pKa = 11.84VIYY77 pKa = 10.19RR78 pKa = 11.84GQPCDD83 pKa = 3.34IKK85 pKa = 11.2AVLPDD90 pKa = 3.29EE91 pKa = 4.93ARR93 pKa = 11.84RR94 pKa = 11.84QHH96 pKa = 6.14VDD98 pKa = 3.2LVVTVGASKK107 pKa = 11.08GG108 pKa = 3.53

Molecular weight: 12.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

11666

50

1051

212.1

23.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.818 ± 0.623

0.9 ± 0.169

5.812 ± 0.226

5.837 ± 0.329

3.12 ± 0.218

8.092 ± 0.36

2.032 ± 0.256

4.089 ± 0.19

3.326 ± 0.355

8.803 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.023 ± 0.119

2.786 ± 0.178

5.306 ± 0.427

4.132 ± 0.309

7.8 ± 0.341

5.357 ± 0.319

5.477 ± 0.345

6.84 ± 0.354

1.826 ± 0.188

2.623 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski