Clostridium sp. CAG:58

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2660 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6RLV8|R6RLV8_9CLOT Branched-chain amino acid transport system carrier protein OS=Clostridium sp. CAG:58 OX=1262824 GN=BN719_02356 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 10.17KK3 pKa = 10.45KK4 pKa = 10.63IMTLVLTTAMTAAAGLTAFAGEE26 pKa = 4.34WKK28 pKa = 10.9SNDD31 pKa = 3.67TGWWWLNDD39 pKa = 3.87DD40 pKa = 4.54GSNPAAEE47 pKa = 4.48WKK49 pKa = 9.65WLDD52 pKa = 3.5GNKK55 pKa = 10.49DD56 pKa = 3.56GTFEE60 pKa = 4.35CYY62 pKa = 10.54YY63 pKa = 10.2FGNDD67 pKa = 3.66GYY69 pKa = 10.3MLSDD73 pKa = 3.05TTTPDD78 pKa = 2.83NYY80 pKa = 10.53QVNKK84 pKa = 10.2DD85 pKa = 3.67GAWIVDD91 pKa = 3.58GGVQLQFNLEE101 pKa = 4.01EE102 pKa = 4.02NAGNDD107 pKa = 3.62EE108 pKa = 4.16EE109 pKa = 6.16DD110 pKa = 4.12GEE112 pKa = 4.53NSGEE116 pKa = 3.73ASYY119 pKa = 10.96EE120 pKa = 3.64YY121 pKa = 10.43DD122 pKa = 3.59AYY124 pKa = 11.14DD125 pKa = 5.11LIGTYY130 pKa = 8.39TSEE133 pKa = 4.93DD134 pKa = 3.38GDD136 pKa = 3.96YY137 pKa = 10.91MEE139 pKa = 6.19LNLGADD145 pKa = 3.77DD146 pKa = 4.75SLVGMYY152 pKa = 10.19YY153 pKa = 10.56DD154 pKa = 4.38EE155 pKa = 6.18DD156 pKa = 4.53GSFDD160 pKa = 4.1GIYY163 pKa = 10.43SFKK166 pKa = 10.61KK167 pKa = 10.46VNSLEE172 pKa = 4.34YY173 pKa = 10.22KK174 pKa = 10.56DD175 pKa = 4.78SLHH178 pKa = 6.27SRR180 pKa = 11.84SIKK183 pKa = 10.15FSNEE187 pKa = 2.91GSFTMRR193 pKa = 11.84DD194 pKa = 3.06TVYY197 pKa = 10.88YY198 pKa = 10.3RR199 pKa = 3.59

Molecular weight: 22.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6S4K1|R6S4K1_9CLOT SpoIID/LytB domain OS=Clostridium sp. CAG:58 OX=1262824 GN=BN719_00370 PE=4 SV=1
MM1 pKa = 7.5GGHH4 pKa = 6.53RR5 pKa = 11.84LSRR8 pKa = 11.84HH9 pKa = 4.56LHH11 pKa = 5.89IDD13 pKa = 3.08PLVIRR18 pKa = 11.84RR19 pKa = 11.84HH20 pKa = 5.25RR21 pKa = 11.84RR22 pKa = 11.84IQGRR26 pKa = 11.84RR27 pKa = 11.84HH28 pKa = 4.84SRR30 pKa = 11.84IVDD33 pKa = 3.95RR34 pKa = 11.84FRR36 pKa = 11.84SLSQRR41 pKa = 11.84VHH43 pKa = 6.84AGQINVILCLILQPLKK59 pKa = 10.57LIPVLPDD66 pKa = 2.82IRR68 pKa = 11.84DD69 pKa = 3.42LRR71 pKa = 11.84VRR73 pKa = 11.84SLGVAVLPVIQVIAVCIHH91 pKa = 6.9HH92 pKa = 7.19ILPADD97 pKa = 4.54GKK99 pKa = 9.39PLIPGCHH106 pKa = 6.14RR107 pKa = 11.84DD108 pKa = 2.85IFRR111 pKa = 11.84CRR113 pKa = 11.84RR114 pKa = 11.84KK115 pKa = 10.03AGRR118 pKa = 11.84PGLSHH123 pKa = 6.08SLCRR127 pKa = 11.84RR128 pKa = 11.84ALPGPVHH135 pKa = 6.38RR136 pKa = 11.84TDD138 pKa = 4.32LDD140 pKa = 4.45LIGISIGQRR149 pKa = 11.84PVSQAFCPEE158 pKa = 3.34FRR160 pKa = 11.84LIRR163 pKa = 11.84LEE165 pKa = 4.09GLCHH169 pKa = 7.22LVFLTVDD176 pKa = 2.8SMDD179 pKa = 3.08RR180 pKa = 11.84HH181 pKa = 5.49LVARR185 pKa = 11.84HH186 pKa = 6.14IGLSPPGHH194 pKa = 5.86GKK196 pKa = 10.1GRR198 pKa = 11.84VPVLHH203 pKa = 6.45LHH205 pKa = 6.06PLRR208 pKa = 11.84RR209 pKa = 11.84CHH211 pKa = 6.82FRR213 pKa = 11.84LRR215 pKa = 11.84DD216 pKa = 3.7PFLHH220 pKa = 6.4VPQEE224 pKa = 3.81LHH226 pKa = 6.53LCNIHH231 pKa = 6.41QGSVPQGPADD241 pKa = 3.92LNGHH245 pKa = 6.55LLHH248 pKa = 7.04GCLALQIDD256 pKa = 4.77DD257 pKa = 5.41DD258 pKa = 4.71GASLAYY264 pKa = 10.06GLLFSCEE271 pKa = 4.07KK272 pKa = 9.48FQKK275 pKa = 10.29RR276 pKa = 11.84PSQAPFLYY284 pKa = 10.4FSVFDD289 pKa = 4.41LKK291 pKa = 11.42DD292 pKa = 3.28LFFSLFCLHH301 pKa = 6.82RR302 pKa = 11.84NSSVHH307 pKa = 5.98NGVARR312 pKa = 11.84AVHH315 pKa = 6.23RR316 pKa = 11.84VDD318 pKa = 5.49DD319 pKa = 4.47EE320 pKa = 4.82PGHH323 pKa = 5.89GLSGAKK329 pKa = 9.59LRR331 pKa = 11.84RR332 pKa = 11.84NGHH335 pKa = 6.7RR336 pKa = 11.84ILPGNGLLTFQGKK349 pKa = 9.86AGRR352 pKa = 11.84TGKK355 pKa = 9.84QLLHH359 pKa = 6.95RR360 pKa = 11.84SLGDD364 pKa = 3.28LRR366 pKa = 11.84VAGGRR371 pKa = 11.84RR372 pKa = 11.84MDD374 pKa = 5.46LFLCDD379 pKa = 3.48PHH381 pKa = 8.78GPDD384 pKa = 5.1LPGSPYY390 pKa = 9.97IFLIILCKK398 pKa = 8.94TCYY401 pKa = 9.76VRR403 pKa = 11.84QIHH406 pKa = 5.83LTVAVQIGPVIKK418 pKa = 10.43QIVMDD423 pKa = 4.67LAGGCQLQKK432 pKa = 11.18LLLIVLIHH440 pKa = 6.6SSAAVDD446 pKa = 3.3IAHH449 pKa = 6.49GQNADD454 pKa = 3.56AASGSALPVSVVFRR468 pKa = 11.84RR469 pKa = 11.84IVHH472 pKa = 6.26

Molecular weight: 52.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2660

0

2660

805197

29

2543

302.7

33.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.423 ± 0.053

1.477 ± 0.02

5.452 ± 0.037

7.688 ± 0.063

4.084 ± 0.03

8.12 ± 0.047

1.74 ± 0.019

6.373 ± 0.046

5.845 ± 0.043

9.239 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.284 ± 0.026

3.545 ± 0.031

3.805 ± 0.027

2.979 ± 0.025

5.242 ± 0.039

5.694 ± 0.042

5.232 ± 0.032

7.071 ± 0.041

0.97 ± 0.018

3.727 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski