Clostridium uliginosum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3511 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I1Q8H2|A0A1I1Q8H2_9CLOT Bifunctional chorismate mutase/prephenate dehydratase OS=Clostridium uliginosum OX=119641 GN=SAMN05421842_12236 PE=4 SV=1
MM1 pKa = 7.32YY2 pKa = 10.55SDD4 pKa = 5.69DD5 pKa = 4.66LLDD8 pKa = 4.53SIRR11 pKa = 11.84IYY13 pKa = 9.47QFCAVEE19 pKa = 4.63LNLFLDD25 pKa = 3.92NFPDD29 pKa = 3.88NEE31 pKa = 4.19NAKK34 pKa = 10.55DD35 pKa = 3.73DD36 pKa = 4.1YY37 pKa = 10.57KK38 pKa = 10.94TVSGKK43 pKa = 7.8LTCLINEE50 pKa = 4.16YY51 pKa = 10.31EE52 pKa = 4.23EE53 pKa = 5.18NYY55 pKa = 10.79GPLTSFGSSFIEE67 pKa = 5.34DD68 pKa = 3.15PRR70 pKa = 11.84AWTEE74 pKa = 4.33QAWPWEE80 pKa = 4.17NYY82 pKa = 8.9EE83 pKa = 4.52KK84 pKa = 11.28EE85 pKa = 4.22EE86 pKa = 4.14

Molecular weight: 10.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I1QFI3|A0A1I1QFI3_9CLOT Glycosyl hydrolases family 8 OS=Clostridium uliginosum OX=119641 GN=SAMN05421842_12324 PE=3 SV=1
MM1 pKa = 7.59SRR3 pKa = 11.84EE4 pKa = 4.09DD5 pKa = 3.24NNRR8 pKa = 11.84RR9 pKa = 11.84SGGRR13 pKa = 11.84VRR15 pKa = 11.84RR16 pKa = 11.84SKK18 pKa = 10.9RR19 pKa = 11.84KK20 pKa = 9.15VCAFCADD27 pKa = 3.46KK28 pKa = 11.38AEE30 pKa = 4.6SIDD33 pKa = 3.6YY34 pKa = 10.94KK35 pKa = 11.04DD36 pKa = 3.2INKK39 pKa = 9.06LRR41 pKa = 11.84KK42 pKa = 9.21YY43 pKa = 7.83VTEE46 pKa = 4.22RR47 pKa = 11.84GKK49 pKa = 10.15ILPRR53 pKa = 11.84RR54 pKa = 11.84ISGTCAKK61 pKa = 9.93HH62 pKa = 4.41QRR64 pKa = 11.84QVTEE68 pKa = 4.13SIKK71 pKa = 9.92RR72 pKa = 11.84ARR74 pKa = 11.84NIALLPFTTEE84 pKa = 3.59

Molecular weight: 9.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3511

0

3511

1079236

26

3149

307.4

34.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.421 ± 0.045

1.233 ± 0.016

5.632 ± 0.034

7.344 ± 0.043

4.306 ± 0.031

6.317 ± 0.044

1.316 ± 0.013

10.202 ± 0.054

9.312 ± 0.04

9.092 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.582 ± 0.018

6.67 ± 0.046

2.624 ± 0.023

2.41 ± 0.023

3.06 ± 0.025

6.424 ± 0.033

5.026 ± 0.032

6.276 ± 0.033

0.679 ± 0.014

4.075 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski