Hubei virga-like virus 16

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KJI0|A0A1L3KJI0_9VIRU Uncharacterized protein OS=Hubei virga-like virus 16 OX=1923331 PE=4 SV=1
MM1 pKa = 7.23VSTVLSLLNIVCLFSFGGSGFASPLVFGSNITLTDD36 pKa = 3.74FNVLLDD42 pKa = 3.87RR43 pKa = 11.84FKK45 pKa = 11.31EE46 pKa = 3.97LDD48 pKa = 3.56DD49 pKa = 4.14TVTSLAVTAVSMEE62 pKa = 4.1SMVSDD67 pKa = 4.88FIMNFQKK74 pKa = 11.07LLLQTGTHH82 pKa = 6.44FNDD85 pKa = 3.31TLIFSEE91 pKa = 4.14KK92 pKa = 9.85FYY94 pKa = 11.03NVSMQFNSLHH104 pKa = 6.31ASILTVLKK112 pKa = 9.57TLEE115 pKa = 4.13LSTTKK120 pKa = 10.38SANLSFVKK128 pKa = 10.08TFLYY132 pKa = 10.01GPKK135 pKa = 9.91GIAYY139 pKa = 8.09SIPKK143 pKa = 10.07ASYY146 pKa = 10.03QSFKK150 pKa = 10.63RR151 pKa = 11.84PSSPYY156 pKa = 8.53VTTHH160 pKa = 6.87AYY162 pKa = 8.34TSLFAGLLGDD172 pKa = 4.13IEE174 pKa = 4.5RR175 pKa = 11.84LVVNSFVYY183 pKa = 10.2VLKK186 pKa = 10.95AFLQIFIDD194 pKa = 3.89SLKK197 pKa = 10.94LIVDD201 pKa = 3.54EE202 pKa = 4.41LLRR205 pKa = 11.84VLRR208 pKa = 11.84NMLPVFEE215 pKa = 5.3RR216 pKa = 11.84LVLMLVDD223 pKa = 5.09IVQQLLKK230 pKa = 10.94LLTEE234 pKa = 4.94LFDD237 pKa = 4.82KK238 pKa = 11.25LNEE241 pKa = 4.0QYY243 pKa = 11.4FMVEE247 pKa = 3.56LVIVFGLCYY256 pKa = 10.41YY257 pKa = 7.47FTRR260 pKa = 11.84DD261 pKa = 2.73IYY263 pKa = 10.24ITIGLFLIFFSIFDD277 pKa = 3.67LTKK280 pKa = 10.55

Molecular weight: 31.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KJH5|A0A1L3KJH5_9VIRU Helicase OS=Hubei virga-like virus 16 OX=1923331 PE=4 SV=1
MM1 pKa = 7.46NLRR4 pKa = 11.84MSTAPDD10 pKa = 3.23TVVAGKK16 pKa = 9.5PSQAKK21 pKa = 9.98VISATDD27 pKa = 3.76GAQVTRR33 pKa = 11.84AGQRR37 pKa = 11.84LEE39 pKa = 4.99DD40 pKa = 4.85PIGTAIMSCYY50 pKa = 10.42SNLVYY55 pKa = 10.8SPLIVVLYY63 pKa = 9.71VFALSCTAASIFGVEE78 pKa = 4.53GPLEE82 pKa = 3.95FAADD86 pKa = 4.94EE87 pKa = 4.09IQKK90 pKa = 9.43TYY92 pKa = 9.86NTTKK96 pKa = 10.7APAVKK101 pKa = 9.97ALAGASYY108 pKa = 10.58RR109 pKa = 11.84IVKK112 pKa = 9.64YY113 pKa = 9.98VLVYY117 pKa = 7.63QTVAITVCLVWLPYY131 pKa = 10.51AKK133 pKa = 10.28KK134 pKa = 10.08PSSKK138 pKa = 10.55NFNASVLFTVLSFLISGLGLLEE160 pKa = 5.18LFLLTQCWFLYY171 pKa = 10.33TEE173 pKa = 4.68LRR175 pKa = 11.84NPRR178 pKa = 11.84HH179 pKa = 6.07KK180 pKa = 10.86LFVAAFVGFLIIFQYY195 pKa = 10.09MADD198 pKa = 3.84PAQGQITNKK207 pKa = 9.38PKK209 pKa = 10.52RR210 pKa = 11.84IYY212 pKa = 10.12HH213 pKa = 5.37YY214 pKa = 10.77NWPTSSKK221 pKa = 9.91EE222 pKa = 3.87IEE224 pKa = 4.02FSPVKK229 pKa = 10.26FSKK232 pKa = 10.46PVVIEE237 pKa = 4.21TQTTTVFSQPVTPASTTSEE256 pKa = 4.1LANVPVKK263 pKa = 10.55HH264 pKa = 6.93SSFPYY269 pKa = 9.81SKK271 pKa = 10.69RR272 pKa = 11.84PDD274 pKa = 3.53GTSSLSRR281 pKa = 11.84SDD283 pKa = 3.21MPQIIFEE290 pKa = 4.37HH291 pKa = 5.81NRR293 pKa = 11.84RR294 pKa = 11.84KK295 pKa = 10.03NLRR298 pKa = 11.84QLPTDD303 pKa = 3.58PP304 pKa = 5.06

Molecular weight: 33.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

4133

117

2971

688.8

77.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.928 ± 0.734

2.008 ± 0.298

5.638 ± 1.217

5.444 ± 0.663

5.783 ± 1.971

5.347 ± 0.924

2.057 ± 0.42

4.936 ± 0.883

5.105 ± 0.382

10.38 ± 1.73

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.96 ± 0.296

3.654 ± 0.369

4.379 ± 0.601

2.976 ± 0.366

5.807 ± 0.815

7.404 ± 0.759

6.267 ± 0.394

9.654 ± 0.644

0.605 ± 0.151

4.67 ± 0.213
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski