Arthrobacter phage Tophat

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Klausavirus; unclassified Klausavirus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 111 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A286N420|A0A286N420_9CAUD Uncharacterized protein OS=Arthrobacter phage Tophat OX=2015838 GN=7 PE=4 SV=1
MM1 pKa = 7.14AQITFEE7 pKa = 4.11EE8 pKa = 3.98ARR10 pKa = 11.84AAVRR14 pKa = 11.84AQNQPIWEE22 pKa = 4.83DD23 pKa = 2.88NDD25 pKa = 4.04TPGEE29 pKa = 4.16YY30 pKa = 9.22MVADD34 pKa = 3.96YY35 pKa = 10.68GWEE38 pKa = 3.88NDD40 pKa = 3.15EE41 pKa = 5.31SYY43 pKa = 11.63LLVEE47 pKa = 4.83GARR50 pKa = 11.84EE51 pKa = 3.85YY52 pKa = 11.53LEE54 pKa = 6.08DD55 pKa = 4.51GDD57 pKa = 4.14NEE59 pKa = 4.4YY60 pKa = 11.07VIQDD64 pKa = 3.58APAVFVDD71 pKa = 4.19KK72 pKa = 10.32EE73 pKa = 4.34TGTVVLAEE81 pKa = 4.05FLAVFEE87 pKa = 5.5VIASMTPVPGHH98 pKa = 6.6KK99 pKa = 10.16PPTDD103 pKa = 3.5TDD105 pKa = 3.68DD106 pKa = 5.55DD107 pKa = 4.17EE108 pKa = 4.74

Molecular weight: 12.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A286N444|A0A286N444_9CAUD Lysozyme OS=Arthrobacter phage Tophat OX=2015838 GN=31 PE=3 SV=1
MM1 pKa = 7.38KK2 pKa = 10.42KK3 pKa = 10.33EE4 pKa = 3.69IGIGLGVMLLAATAFLWSEE23 pKa = 3.94HH24 pKa = 6.08SSFTVILLVILVGSLAVLTIYY45 pKa = 10.71YY46 pKa = 8.42MRR48 pKa = 11.84RR49 pKa = 11.84SHH51 pKa = 5.43WRR53 pKa = 11.84RR54 pKa = 11.84YY55 pKa = 7.78PSGRR59 pKa = 11.84VFMYY63 pKa = 9.06LIWAFDD69 pKa = 3.6ALIVYY74 pKa = 8.37WLFSRR79 pKa = 11.84LIEE82 pKa = 4.78SRR84 pKa = 11.84DD85 pKa = 3.38LRR87 pKa = 11.84IGIFNVLIAGLVAAIWLITGTFWKK111 pKa = 9.54SQRR114 pKa = 11.84RR115 pKa = 11.84ARR117 pKa = 11.84AEE119 pKa = 3.83RR120 pKa = 11.84LRR122 pKa = 11.84NAAITKK128 pKa = 10.16KK129 pKa = 10.82EE130 pKa = 3.91EE131 pKa = 4.06TQPP134 pKa = 3.46

Molecular weight: 15.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

111

0

111

22359

35

1178

201.4

21.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.982 ± 0.39

0.957 ± 0.145

5.962 ± 0.163

6.23 ± 0.274

2.831 ± 0.139

8.31 ± 0.322

2.254 ± 0.188

4.079 ± 0.192

4.468 ± 0.249

7.518 ± 0.232

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.473 ± 0.112

3.676 ± 0.128

6.011 ± 0.238

3.426 ± 0.133

6.105 ± 0.274

5.09 ± 0.197

6.932 ± 0.22

7.366 ± 0.232

1.834 ± 0.124

2.496 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski