Staphylococcus phage vB_SscM-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Twortvirinae; Sciuriunavirus; unclassified Sciuriunavirus

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 202 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9I9U9|A0A1X9I9U9_9CAUD Uncharacterized protein OS=Staphylococcus phage vB_SscM-2 OX=1868845 GN=vB_SscM-2_004 PE=4 SV=1
MM1 pKa = 7.51NKK3 pKa = 9.22EE4 pKa = 3.65QYY6 pKa = 10.69NYY8 pKa = 10.52LFNKK12 pKa = 7.41VTVFKK17 pKa = 11.24DD18 pKa = 3.14MEE20 pKa = 4.95DD21 pKa = 3.99YY22 pKa = 11.24NNKK25 pKa = 9.21TIDD28 pKa = 3.49GAEE31 pKa = 4.01QLDD34 pKa = 3.9EE35 pKa = 4.33ALQEE39 pKa = 4.77GFIQDD44 pKa = 3.9LTRR47 pKa = 11.84GVYY50 pKa = 10.28NGATDD55 pKa = 3.79TFTPNTLNAKK65 pKa = 9.32EE66 pKa = 4.21LVLAVQQYY74 pKa = 10.9LYY76 pKa = 9.44EE77 pKa = 4.38TDD79 pKa = 3.43WLSTLYY85 pKa = 9.2TQHH88 pKa = 6.93EE89 pKa = 4.38EE90 pKa = 4.5DD91 pKa = 5.21DD92 pKa = 4.03DD93 pKa = 5.6LEE95 pKa = 4.38TFEE98 pKa = 4.28QLTRR102 pKa = 11.84FDD104 pKa = 3.68MLEE107 pKa = 4.65DD108 pKa = 3.6YY109 pKa = 10.9LNCYY113 pKa = 10.31SVFTIGNRR121 pKa = 11.84IYY123 pKa = 11.47ADD125 pKa = 3.08ISS127 pKa = 3.42

Molecular weight: 14.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9IAA5|A0A1X9IAA5_9CAUD Uncharacterized protein OS=Staphylococcus phage vB_SscM-2 OX=1868845 GN=vB_SscM-2_091 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 10.39NRR4 pKa = 11.84NGRR7 pKa = 11.84NNIQHH12 pKa = 5.74QPVNFAPTNLLGGTGNNSFYY32 pKa = 10.67KK33 pKa = 10.3KK34 pKa = 10.44KK35 pKa = 10.32PGEE38 pKa = 4.37HH39 pKa = 6.77KK40 pKa = 10.76DD41 pKa = 3.36GAKK44 pKa = 9.87TIVYY48 pKa = 9.97KK49 pKa = 11.05LLFTKK54 pKa = 10.59RR55 pKa = 11.84FDD57 pKa = 3.66YY58 pKa = 11.04VSQKK62 pKa = 10.39DD63 pKa = 3.16IQMQKK68 pKa = 10.41KK69 pKa = 9.14YY70 pKa = 10.74ALNLISDD77 pKa = 3.81SLGIKK82 pKa = 10.26EE83 pKa = 4.52EE84 pKa = 3.98FLTLKK89 pKa = 10.65QKK91 pKa = 10.16GKK93 pKa = 9.86KK94 pKa = 9.28VEE96 pKa = 4.78EE97 pKa = 4.02ILHH100 pKa = 5.78TDD102 pKa = 2.76RR103 pKa = 11.84VFYY106 pKa = 8.54VHH108 pKa = 7.28RR109 pKa = 11.84GKK111 pKa = 10.87KK112 pKa = 10.35LIGKK116 pKa = 9.5CSIRR120 pKa = 11.84EE121 pKa = 3.65QRR123 pKa = 11.84TFKK126 pKa = 9.73GTHH129 pKa = 6.73LIYY132 pKa = 10.67VFKK135 pKa = 10.32TRR137 pKa = 11.84HH138 pKa = 4.77RR139 pKa = 11.84TSGKK143 pKa = 10.38KK144 pKa = 9.8KK145 pKa = 9.84GQQ147 pKa = 3.24

Molecular weight: 17.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

202

0

202

41063

37

1321

203.3

23.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.135 ± 0.177

0.594 ± 0.067

6.673 ± 0.147

8.808 ± 0.271

3.921 ± 0.107

5.891 ± 0.305

1.617 ± 0.104

7.411 ± 0.197

8.993 ± 0.255

8.044 ± 0.197

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.521 ± 0.106

6.322 ± 0.126

2.771 ± 0.159

3.331 ± 0.164

3.806 ± 0.11

6.359 ± 0.162

6.198 ± 0.217

6.592 ± 0.16

0.852 ± 0.057

5.16 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski